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  • 1
    Electronic Resource
    Electronic Resource
    Evaluation of possible horizontal gene transfer from transgenic plants to the soil bacterium Acinetobacter calcoaceticus BD413 (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 815-821 
    Details
    ISSN: 1432-2242
    Keywords: Key words Risk assessment ; Horizontal gene transfer ; Transgenic plants ; Natural transformation ; Soil bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The use of genetically engineered crop plants has raised concerns about the transfer of their engineered DNA to indigenous microbes in soil. We have evaluated possible horizontal gene transfer from transgenic plants by natural transformation to the soil bacterium Acinetobacter calcoaceticus BD413. The transformation frequencies with DNA from two sources of transgenic plant DNA and different forms of plasmid DNA with an inserted kanamycin resistance gene, nptII, were measured. Clear effects of homology were seen on transformation frequencies, and no transformants were ever detected after using transgenic plant DNA. This implied a transformation frequency of less than 10-13 (transformants per recipient) under optimised conditions, which is expected to drop even further to a minimum of 10-16 due to soil conditions and a lowered concentration of DNA available to cells. Previous studies have shown that chromosomal DNA released to soil is only available to A. calcoaceticus for limited period of time and that A. calcoaceticus does not maintain detectable competence in soil. Taken together, these results suggest that A. calcoaceticus does not take up non-homologous plant DNA at appreciable frequencies under natural conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050630
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  • 2
    Electronic Resource
    Electronic Resource
    Spatial variation of biochemical and ecological phenotypes in Drosophila: Electrophoretic and quantitative variationBased on a lecture delivered in September 1983, at the International Conference in Biochemical and Development Genetics, Kos, Greece. (1983)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 4 (1983), S. 439-450 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; electrophoretic variation ; quantitative variation ; ecology ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Electrophoretic variation at three enzyme loci-alcohol dehydrogenase (Adh), glycerophosphate dehydrogenase (Gpdh), triosephosphate isomerase (Tpi)- is compared in Australian Drosophila melanogaster populations at three levels of spatial heterogeneity; among breeding sites, within populations, and between populations at the geographic level. Heterogeneity at the breeding site level greatly exceeds that among adults within populations, indicating greater intermixing at the mobile adult stage than at the developmentally immature and less migratory larval stage. Heterogeneity at the microspatial level is large relative to the geographic level at two of these loci.Spatial patterns of variation in ecological phenotypes are also considered. It is argued that electrophoretic variants may contribute little to an understanding of this quantitative variation, and that a more useful approach in ecological genetics is to consider ecological phenotypes as primary data.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020040416
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    Paper (German National Licenses)
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