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  • 1
    Electronic Resource
    Electronic Resource
    Fibroblast culture in Werner's syndrome (1971)
    Springer
    Human genetics 〈Berlin〉 13 (1971), S. 244-246 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei Fibroblasten einer Patientin mit einem Werner-Syndrom und normalen Fibroblasten wurden die Reproduktionsrate und das Aufnahmevermögen für 3H-Thymidin in vitro verglichen. Beim Werner-Syndrom war die 3H-Aufnahme niedriger als bei den normalen Kontrollzellen.
    Notes: Summary Growth capacities of fibroblasts from a woman with Werner's syndrome and of normal fibroblasts are compared by means of subculture-counting and 3H-thymidineuptake determination. The cells from the patient showed lower 3H-incorporation values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326950
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Time and cell systems as variables in fusion experiments with polyethylene glycol (1978)
    Springer
    Human genetics 〈Berlin〉 42 (1978), S. 319-322 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291313
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A straightforward approach to isolate DNA sequences with potential linkage to the retinoblastoma locus (1986)
    Springer
    Human genetics 〈Berlin〉 74 (1986), S. 249-255 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary From a human-Chinese hamster somatic cell hybrid a clone was derived containing chromosome 13 in duplicate as its only human material. This clone was used to construct a human chromosome 13-specific recombinant DNA-library. Overlapping Sau3AI DNA sequences (11.9–17.2 kb) from the cell hybrid were inserted into the lambda phage vector EMBL4. From eleven recombinants having a human insert thirteen putative unique DNA sequences were isolated and cloned into the plasmid vector pBR329. A human-mouse hybrid containing a human chromosome 13 with a deletion of 13q14 and lacking its undeleted homologue was constructed to be used in a selection procedure for DNA sequences belonging to band q14. Three probes originating from two different phages were assigned to 13q14 because they did not hybridise to DNA from this cell hybrid. One of these 13q14 probes detects a low frequency (2/44) Msp I restriction fragment length polymorphism. The probes are now being used for screening a cosmid library to find adjacent polymorphic sequences with a RFLP information content suitable for application in the diagnosis of hereditary retinoblastoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282543
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Satellite associations and the identification of the Y chromosome in man (1963)
    Springer
    Genetica 33 (1963), S. 45-51 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a sample of cells from a leucocyte culture of an XY/XO mosaic the number of satellite associations between two acrocentric chromosomes (groups 13–15 and 21–22) was determined. Three different types of associations were distinguished. A comparison of the numerical frequencies of the different types in XO and XY cells suggests that the Y chromosome does not take part in the associations and is therefore unsatellited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01725746
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    Paper (German National Licenses)
    Fulltext
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