ISSN:
1573-5044
Keywords:
callus culture
;
electrophoresis
;
soluble protein
;
somaticembryogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Plant regeneration from cultured immature inflorescence segments (3–5 mm) of sugarcane (Saccharum sp) var. CP 5243 was obtained via somatic embryogenesis. Embryogenic callus culture was initiated on MS medium supplemented with 2,4-D (13.5 µM) over 30 days. The callus was subcultured every 15–20 days on MS medium supplemented with 2,4-D (4.5 µM), arginine (50 mg l-1) and proline (500 mg l-1). The callus was subjected to five treatments: 2,4-D (4.5 µM), Picloram (8.2 µM) and Dicamba (22.6 µM). SPC was determined at the beginning, after 20 days in culture, and every 24 hours thereafter up to 72 hours. SDS-PAGE electrophoresis was performed based on soluble protein content. Some differences were found between SPC and bands (intensity and number) for all treatments associated with shoot formation. The results point out the association of soluble protein content and callus regenerative ability of sugarcane cv. CP5243 and suggest the presence of a marker protein (between 55–70 kDa) for embryogenic callus regeneration ability in this cultivar.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005963925773
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