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  • 1
    Electronic Resource
    Electronic Resource
    Protein-based capillary affinity gel electrophoresis for the separation of optical isomers (1992)
    s.l. : American Chemical Society
    Analytical chemistry 64 (1992), S. 2872-2874 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00046a038
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  • 2
    Electronic Resource
    Electronic Resource
    Genital cancer among wives of men with penile cancer. A study between 1958 and 1982 (1989)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 96 (1989), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Previous studies have suggested an association between penile cancer and cervical cancer but the results have been difficult to interpret. The current study includes 1064 cases of penile cancer reported to the National Swedish Cancer Registry between 1958 and 1980. One matched control for each living case was chosen randomly from the Swedish Population Registry, and for cases who had died, from the National Death Registry. Details about the men, their marriages and each wife or wives were obtained from the population registry in the parish in which the men lived or had died. The personal identification number and name of each wife or wives were matched against the cancer registry. All genital cancers, including 15 cervical cancers, were distributed equally among the wives of the cases and those of the controls. The expected rate of cancers among the population of wives of cases was also calculated and there was no difference between the observed and the expected number of women with cancer. The results did not show an association between penile cancer in men and cervical cancer in their sexual partners.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1989.tb01666.x
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  • 3
    Electronic Resource
    Electronic Resource
    Thin-Layer Immunoaffinity Chromatography with Bar Code Quantitation of C-Reactive Protein (1995)
    s.l. : American Chemical Society
    Analytical chemistry 67 (1995), S. 3051-3056 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00113a046
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  • 4
    Electronic Resource
    Electronic Resource
    Incidence of CSF abnormalities in siblings of multiple sclerosis patients and unrelated controls (2000)
    Springer
    Journal of neurology 247 (2000), S. 616-622 
    Details
    ISSN: 1432-1459
    Keywords: Key words Multiple sclerosis ; Siblings ; Genetics ; Oligoclonal bands ; Measles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We found that 19% (9/47) of healthy siblings of patients with clinically definite multiple sclerosis had an intrathecal immunological reaction with two or more 2 CSF-enriched oligoclonal bands (OCBs), in contrast to (4%) (2/50) unrelated healthy controls. Furthermore, in this group of nine healthy sibs the measles CSF IgG antibody titers were higher than that of the other sibs and that of controls. There were also differences in the serum titers for measles IgG antibody, which were higher in the group of all healthy sibs than in healthy volunteers, and (as with CSF titers) higher in the subgroup of healthy sibs with two or more 2 CSF-enriched OCBs than the other sibs. Thus a significant proportion of healthy siblings to MS patients have a partially hyperimmune condition similar to that occurring in MS, which in 19% manifested itself as an OCB reaction, in 9% as increased CSF measles IgG antibody titers, and in 21% as increased serum measles IgG antibody titers, these phenomena tending to occur in the same individuals. This condition is characterized by CSF-enriched OCBs with undefined specificity, although some increased antiviral reactivity is found both in the serum and CSF. While it needs further characterization, a genetic trait interacting with common infections is suggested. The recurrence risk of this condition is approximately five times higher than the 3–4% recurrence risk for manifest MS reported for sibs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150070130
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  • 5
    Electronic Resource
    Electronic Resource
    Sexual behavior of women with repeated episodes of vulvovaginal candidiasis (1995)
    Springer
    European journal of epidemiology 11 (1995), S. 575-579 
    Details
    ISSN: 1573-7284
    Keywords: Sexual behavior ; Sexually transmitted disease (STD) ; Vulvovaginal candidiasis (VVC)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One hundred and two women with a history of a median of six episodes of vulvovaginal candidiasis (VVC) and 204 age-matched controls participated in a structured in-depth interview on sexual behavior. Mean and median ages of the two groups were 26.7 and 26 years, respectively. Sexual characteristics, associated with VVC in crude analyses, were adjusted in multifactorial analyses for coital frequency, experience of casual sex, vaginal irritation, smoking, alcohol habits, and having a steady partner. In addition, education, as a measure of socioeconomic status, was added in the multifactorial analyses. VVC was not associated with multiple sexual partners or ever-experience of causal sex. Sexual variables that remained significant or were of borderline significance after adjustment were: age at first intercourse (p=0.001), casual sex partners the previous month (odds ratio (OR)=3.1), sex during menstruation (OR=1.7), regular oral sex (OR=2.4), experience of anal intercourse ever (OR=2.4), oral intercourse the last month (OR=3.1), and frequency of oral intercourse (p=0.02). Thus, the study indicates that certain sexual activities are associated with repeated episodes of VVC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01719311
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  • 6
    Electronic Resource
    Electronic Resource
    Sexual risk behavior in women with cervical human papillomavirus infection (1996)
    Springer
    Archives of sexual behavior 25 (1996), S. 361-372 
    Details
    ISSN: 1573-2800
    Keywords: human papillomavirus ; cervix uteri ; sexual behavior ; sexually transmitted disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Psychology
    Notes: Abstract In a study of 972 women, sexual characteristics of 66 women with a cervical human papillomavirus infection (CHPI) were compared to the remaining study population. Among a number of sexual variables that were significantly correlated with CHPI were number of lifetime sexual partners, short partnerships, many recent partners, infidelity, casual travel sex, sexual début abroad, oral and anal sex, and sexual abuse. In multifactorial analyses four variables remained significantly correlated with CHPI, i.e., number of lifetime sexual partners, casual travel sex, sexual début abroad, and infidelity. It is concluded that CHPI shows most of the epidemiological characteristics of a sexually transmitted disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02437579
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  • 7
    Electronic Resource
    Electronic Resource
    Purification of streptococcal protein g expressed by Escherichia coli by high performance liquid affinity chromatography using immobilized immunoglobulin G and albuminThis work was supported by the Crafoord Foundation, the Swedish Medical Research Council (proj. no. 3362, 7144, 7486), the Medical Faculty, University of Lund, Gustav V:s 80-year Foundation, Österlunds Foundation, Stiftelsen JO Nauclers Minnesfond and Excorim KB. (1987)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 2 (1987), S. 221-225 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A one-step HPLC method was developed for the purification of protein G, a cell wall molecule from group C and G streptococci with immunoglobulin G- and albumin-binding properties. Lysed Escherichia coli bacteria infected with lambda-phages containing the protein G gene from group G streptococci were used as a starting material for the preparations. The lysate was applied to a column with immobilized human immunoglobulin G or human serum albumin. Protein G was selectively bound and eluted at pH 2.0. A 750-fold purification was achieved. Sodium dodecylsulfate + polyacrylamide gel electrophoresis showed that the highly purified protein G consisted of three sets of doublets with the apparent molecular weight of 64 and 67, 56 and 58, and 45 and 47 kilodaltons, respectively. A specific method for quantitation of small amounts of protein G was developed and used for specific tracing of the protein after the affinity chromatography. Goat polyclonal antibodies were bound to an antigen coated to the plastic walls of microtiter plates, causing the Fc-region of the immunoglobulins to be directed outwards. Unknown samples of protein G were then allowed to compete with radio-iodinated protein G (solid phase radioassay) or protein G coupled to alkaline phosphatase (enzyme linked sorbent assay) for the Fc-regions.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130020510
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  • 8
    Electronic Resource
    Electronic Resource
    Rapid purification of detergent-solubilized bovine hormone-sensitive lipase by high performance hydrophobic interaction chromatography (1989)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 3 (1989), S. 82-87 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Hormone-sensitive lipase (HSL), the enzyme controlling the rate of adipose tissue lipolysis and also possibly involved in the regulation of steroidogenesis, has been purified from bovine omental adipose tissue. Partially detergent-solubilized, delipidated and purified HSL was obtained through step-elution at conventional DEAE ion-exchange chromatography, followed by concentration on hydroxylapatite. High performance hydrophobic interaction chromatography (HPHIC) on phenylsilica then resulted in an increase of HSL protein purity from 2% to more than 70%. Final purification of the enzyme to apparent homogeneity (〉95% protein purity), concentration and removal of most of the detergent was obtained by high performance cation exchange chromatography on Mono S. At least 0.5 mg of highly stable HSL was obtained from 5 kg of bovine omental fat within four working days. The purified lipase had a lower specific activity than previously reported for the corresponding rat enzyme but the preparations have proved very useful for enzyme structure studies and as an antigen.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130030210
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  • 9
    Electronic Resource
    Electronic Resource
    This supplement of Chromatographia contains contributions to the Symposium on High Performance Liquid Phase Microseparations, held in Lund, Sweden, June 7–10, 1998 (1999)
    Springer
    Chromatographia 49 (1999), S. S5 
    Details
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02468969
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  • 10
    Electronic Resource
    Electronic Resource
    Three approaches to enantiomer separation of β-adrenergic antagonists by capillary electrochromatography (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 884-890 
    Details
    ISSN: 0173-0835
    Keywords: Capillary electrophoresis ; Chiral separations ; Cyclodextrins ; Cellobiohydrolase ; Molecular imprinting ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Three different capillary electrochromatographic methods for the enantiomer separation of β-adrenergic antagonists (acebutolol, alprenolol, atenolol, metoprolol, pindolol, prenalterol, and propranolol) were applied using different cyclodextrins (β-cyclodextrin, carboxymethyl-β-cyclodextrin and dimethyl-β-cyclodextrin) added to the electrolyte, a cross-linked protein-gel (cellobiohydrolase I) and a molecularly imprinted ((R)-enantiomer of propranolol) superporous polymer as chiral selectors. Through use of these different separation strategies, all the β-adrenergic antagonists studied could be resolved into their enantiomers, although the three methods were carried out without extensive optimization. The protein and molecularly imprinted phases gave the highest selectivities whereas employing cyclodextrins resulted in the highest separation efficiency. Proteins and cyclodextrins are primarily natural products, albeit the cyclodextrins can be derivatized. In contrast, the molecularly imprinted chiral stationary phase can be highly customized when produced.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180606
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