Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Activation of afferent cardiac sympathetic nerve fibers of the cat by pain producing substances and by noxious heat (1977)
    Springer
    Pflügers Archiv 372 (1977), S. 53-61 
    Details
    ISSN: 1432-2013
    Keywords: Afferent cardiac sympathetic fiber ; Mechanoreceptor ; Pain-producing substance ; Thermal stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Afferent discharges of 64 single units were recorded from the left cardiac sympathetic nerve of anesthetized cats. Mechano-sensitive terminals of the afferent fibers were localized in the extrapulmonary part of the pulmonary artery, left atrium, left ventricle and left pericardium, as determined by direct mechanical probing of the heart after death of the animals. Conduction velocity of the fibers ranged from 2.5 to 14.6 m/s. Excitation of these Aδ-fibers with mechanically excitable endings was produced by intravenous injections of acetylcholine, 5-hydroxytryptamine, bradykinin, histamine and veratridine, and/or by topical application of these agents to the receptor region. Noxious heat to the mechanically excitable field in the wall of the pulmonary artery and the left ventricle also activated their afferent fibers. These observations provide evidence for a certain number of afferent units in the cardiac sympathetic nerve with polymodal sensitivity. These afferent fibers can provide the spinal cord with information not only on mechanical changes in cardiac events, but also changes in the chemical environment of the cardiac nerve ending, possibly produced by myocardial ischemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582206
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Rhythmic Cl− current and physiological roles of the intestinal c-kit-positive cells (1995)
    Springer
    Pflügers Archiv 431 (1995), S. 169-177 
    Details
    ISSN: 1432-2013
    Keywords: c-kit ; Anti-c-kit-monoclonal antibody (ACK2) ; Ca2+-Dependent Cl− current ; Pacemaker activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Chronic injection of an anti-c-KIT receptor tyrosine kinase monoclonal antibody (ACK2) results in the disruption of the normal motility patterns of young BALB/c mice intestine. This effect is accompanied by a drastic decrease in the number of intestinal c-kit-expressing (c-kit +) cells when studied immuno-histochemically with the fluorescence-labelled antibody. In order to clarify the mechanism underlying the ACK2 action and the physiological roles of intestinal c -kit + cells, we studied the excitability of intestinal c -kit + cells in primary culture by use of the nystatin perforated-patch-clamp technique. Under voltageclamp at −40 mV, the majority of c -kif +cells tested (59/70) elicited rhythmic current waves with an amplitude and frequency of 263±24 pA and 2.30±0.25 cycles/min (mean±SEM), respectively. Intracellular perfusion of the c -kit + cells with ethylenebis (okonitrilo) tetraacetate (EGTA) as well as a nominally Ca2+-free external solution or low holding voltage (〈-60 mV) prevented the rhythmic current. The reversal potential of the rhythmic current was close to the equilibrium potential for Cl−(E Cl ) Moreover the rhythmic current was depressed by a Cl− channel blocker, 4-acetoamido-4-isothiocyanat-ostilbene-2,2′-disulphonic acid (SITS). The smooth muscle cells freshly dissociated from the same intestinal specimen revealed a Ca2+-activated K+current, as has been described in a variety of smooth muscle cells. Cultured smooth muscle cells from the ileum preparation lacked neither the Ca2+-activated K+nor rhythmic Cl− currents. Smooth muscle cells freshly dissociated from the same ileum preparation and those in culture showed no immunoreactivity with the labelled ACK2, which was consistent with our previous in situ study. Results provided direct evidence that the intestinal c -kit + cells, but not the smooth muscle cells, possess a rhythmic Cl− current oscillation, suggesting their participation in pacemaker activity for the peristaltic gut movement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410188
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Transmural distribution of myocardial high energy phosphates and lactate in relation to the epicardial ECG in the underperfused canine heart (1981)
    Springer
    Pflügers Archiv 391 (1981), S. 101-104 
    Details
    ISSN: 1432-2013
    Keywords: Coronary flow autoregulation ; Underperfusion ; Epicardial ECG ; Transmural distribution of myocardial metabolites ; Lactate ; High energy phosphates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of coronary perfusion pressure (CPP) on the transmural distribution of myocardial metabolites was examined in the canine heart. Myocardial contents of high energy phosphates and lactate were measured in the inner, middle and outer third of transmural biopsies taken from the underperfused area of the left ventricle; simultaneously epicardial ECG was recorded. Maximum autoregulation of flow was reached when CPP was decreased by approximately 50%; this was indicated by the absence of reactive hyperemia. At this level, high energy phosphate and lactate contents as well as hemodynamic function remained relatively unaltered. At 40% of CPP, coronary flow and the high energy phosphate contents decreased significantly, especially in the subendocardium. At 30% of CPP, there was an accumulation of lactate and a decrease in creatine phosphate content to below one-third of the control in all layers; under these conditions also the ST-segment of the epicardial ECG was elevated. The ST-segment appeared to be relatively insensitive to subendocardial damage; instead, elevation of the ST-segment seemed to be correlated with the content of lactate in the subepicardium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00656998
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    c-kit-Dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract (1995)
    Springer
    Cell & tissue research 280 (1995), S. 97-111 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Gastrointestinal motility ; Enteric nervous system ; Smooth muscle ; Rhythmicity ; Proto-oncogene ; Tyrosine kinase ; Interstitial cells of Cajal ; Mouse (BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In vivo injection of a neutralizing, monoclonal antibody (ACK2) to the receptor tyrosine kinase (c-kit) disrupts the normal motility patterns of the mouse small intestine. Immunohistochemical studies showed that cells expressing c-kit-like immunoreactivity (c-kit-LI) decreased in numbers in response to ACK2, but the identity of these cells is unknown. We investigated the identity and development of the cells that express c-kit-LI in the mou se small intestine and colon. Cells in the region of the myenteric plexus and deep muscular plexus of the small intestine and in the subserosa, in the myenteric plexus region, within the circular and longitudinal muscle layers, and along the submucosal surface of the circular muscle in the colon were labeled with ACK2. The distribution of cells that express c-kit-LI was the same as that of interstitial cells (ICs). In whole-mount preparations cells with c-kit-LI were interconnected, formin g a network similar to the network formed by cells that stained with methylene blue, which has been used as a marker for ICs in the mouse gastrointestinal tract. Immunocytochemistry verified that ICs were labeled with ACK2. Multiple injections of animals with ACK2 between days 0 and 8 post partum (pp) caused a dramatic reduction in the number of ICs compared to control animals. From an ultrastructural point of view, the proliferation and development appeared to be suppressed in some classes of ICs, while ot hers displayed an altered course of development. Functional studies showed that the decrease in ICs was accompanied by a loss of electrical rhythmicity in the small intestine and reduced neural responses in the small bowel and colon. Morphological experiments showed that c-kit-positive cells are ICs, and physiological evidence reinforced the concept that ICs are involved in generation of rhythmicity and translation of neural inputs in gastrointestinal smooth muscles. Controlling the development of ICs provides a powerful new tool for the investigation of the physiological role of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304515
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    c-kit-Dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract (1995)
    Springer
    Cell & tissue research 280 (1995), S. 97-111 
    Details
    ISSN: 1432-0878
    Keywords: Gastrointestinal motility ; Enteric nervous system ; Smooth muscle ; Rhythmicity ; Proto-oncogene ; Tyrosine kinase ; Interstitial cells of Cajal ; Mouse (BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In vivo injection of a neutralizing, monoclonal antibody (ACK2) to the receptor tyrosine kinase (c-kit) disrupts the normal motility patterns of the mouse small intestine. Immunohistochemical studies showed that cells expressing c-kit-like immunoreactivity (c-kit-LI) decreased in numbers in response to ACK2, but the identity of these cells is unknown. We investigated the identity and development of the cells that express c-kit-LI in the mouse small intestine and colon. Cells in the region of the myenteric plexus and deep muscular plexus of the small intestine and in the subserosa, in the myenteric plexus region, within the circular and longitudinal muscle layers, and along the submucosal surface of the circular muscle in the colon were labeled with ACK2. The distribution of cells that express c-kit-LI was the same as that of interstitial cells (ICs). In whole-mount preparations cells with c-kit-LI were interconnected, forming a netword similar to the network formed by cells that stained with methylene blue, which has been used as a marker for ICs in the mouse gastrointestinal tract. Immunocytochemistry verified that ICs were labeled with ACK2. Multiple injections of animals with ACK2 between days 0 and 8 post partum (pp) caused a dramatic reduction in the number of ICs compared to control animals. From an ultrastructural point of view, the proliferation and development appeared to be suppressed in some classes of ICs, while others displayed an altered course of development. Functional studies showed that the decrease in ICs was accompanied by a loss of electrical rhythmicity in the small intestine and reduced neural responses in the small bowel and colon. Morphological experiments showed that c-kit-positive cells are ICs, and physiological evidence reinforced the concept that ICs are involved in generation of rhythmicity and translation of neural inputs in gastrointestinal smooth muscles. Controlling the development of ICs provides a powerful new tool for the investigation of the physiological role of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304515
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Persistent calcium inward current in internally perfused snail neuron (1986)
    Springer
    Cellular and molecular neurobiology 6 (1986), S. 71-85 
    Details
    ISSN: 1573-6830
    Keywords: calcium current ; inactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The inactivation process of the calcium current (I Ca) was investigated in a molluscan neuron which was perfused intracellularly and voltage-clamped using a suction pipette technique. 2. The decay phase of theI Ca contained a very slowly inactivated component (persistent inward current; PIC). The decay time constant of this component was over 10 sec. 3. An increase in the amplitude of theI Ca or the intracellular Ca2+ concentration caused a decrease in the decay time constant of the PIC. 4. Replacing Ba2+ with extracellular Ca2+ increased the decay time constant of the PIC. The differences in the amplitude and the decay kinetics between theI Ca and theI Ba resulted from changes in the amplitude and the decay time constant of the PIC. 5. These observations support the conclusion that the inactivation of the PIC is calcium dependent [Chad, J., Eckert, R., and Ewald, D. (1984).J. Physiol. (Lond.)347:279-300].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00742976
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...