ISSN:
1573-9368
Keywords:
Agrobacterium rhizogenes
;
gene expression
;
GUS staining
;
immunolocalization
;
Nicotiana tabacum
;
rolC gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Expression of therolC gene fromAgrobacterium rhizogenes causes morphological and developmental alterations in transgenic plants. The histological alterations underlying the macroscopic changes and the cellular localization of the site of expression of therolC gene have shown that: (i) the expression of therolC gene is developmentally regulated, (ii) in vegetative transgenic plants, the expression of therolC gene under the control of its own promoter is restricted to companion and protophloem cells, (iii) the site of action of the product(s) of the activity of the rolC enzyme is distinct from its site of expression, (iv) precise localization of the rolC peptide has been achieved by immunocytochemistry but not by the histochemical GUS assay. These results imply that the sites of action and expression of therolC gene in trangenic plants are physically separated. Thus the product(s) of the activity of the rolC enzyme must be a factor capable of being transported. Current models forrolC gene action are discussed taking into account the reported results.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01979917
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