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  • 1
    Electronic Resource
    Electronic Resource
    Morphogenesis of albino rat lung: An autoradiographic analysis of the embryological origin of the type I and II pulmonary epithelial cellsSupported by research Fellowship grants 1-F1-GM-33, 009, 5-F1-GM-33, 009, and Grant-in-Aid GRSG-FR-05403 from the National Institutes of Health. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 69-87 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A correlation of autoradiographic and histochemical data indicates that the type I and II pulmonary epithelial cells are endodermally-derived; and, that the interstitial pulmonary cells are mesodermally-derived. Tritiated thymidine (T-H3) was found to be an excellent cell marker for in vivo developmental studies of mammalian (rat) lung. At a dose of 3 μc per gm (specific activity, 15.6-16.9 c per mM) maternal body weight, T-H3 crosses the placenta in amounts sufficient to effect heavy labeling of dividing cells. A partial placental barrier to T-H3 was found in late stages of development. Following an injection of T-H3 on day 16 of gestation, a higher rate of endodermal cell division was reflected by higher labeling indices and a steeper slope of the endodermal dilution curve as opposed to the mesoderm. This differential in labeling was maintained through the third postnatal day. Neonatal labeling patterns of the definitive cell types (type I and II pulmonary epithelial cells, interstitial pulmonary cells) reflected those of their germ layer precursors.Histochemical analysis of the developing rat lung demonstrated large accumulations of cytoplasmic glycogen in areas of rapid cell division (endodermal cells). As the mitotic rate decreased and cellular differentiation progressed, glycogen decreased; postnatally it is not a feature of mature pulmonary cell types.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051320105
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  • 2
    Electronic Resource
    Electronic Resource
    Fine structural observations of ruthenium red binding in developing and adult rat lungThis study was supported in part by NIH grant HL 15316. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 178 (1974), S. 267-287 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ruthenium red staining technique of Luft ('71b) was utilized in an electron microscopic investigation of developing and adult rat lung. Electron-dense deposits of ruthenium red-positive material were observed on all exposed surfaces of the tissue block, regardless of the stage of development. In the more central areas of the block, sites of ruthenium red binding changed with age. In early prenatal lung (days 16 to 20) dense accumulations of ruthenium red-positive material were found in association with the basement membranes of endodermal epithelial cells. Ruthenium red binding was also observed between adjacent epithelial cells; however, their luminal surfaces were negative. The main intracellular site of ruthenium red binding in intact cells was the lamellar body of the developing type II pulmonary epithelial cells. By day 21 of development, accumulations of granular product were observed in association with most lamellar bodies, as well as on epithelial cell luminal surfaces. Ruthenium red binding in postnatal tissue decreases with increasing age. By the second postnatal week, the predominant site of binding is the luminal surface of the type I and type II pulmonary epithelial cells. When compared to fetal and early neonatal stages, adult rat lung has a still more limited distribution of ruthenium red-positive material. Changes in the distribution of ruthenium red-positive material correlate with numerous morphologic and biochemical events in rat lung development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091780210
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of various fixative-buffer combinations on lung fine structureThis study was supported by NIH grant HL 15316. (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 181 (1975), S. 545-559 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A variety of fixatives, buffers and fixation procedures were compared in rat and squirrel monkey lung in an attempt to preserve optimally both the cytologic details of pulmonary parenchyma as well as the acellular alveolar lining layer. In initial experiments utilizing the fixative of Ito and Karnovsky ('68), an electron-dense deposit was observed on the alveolar surface. Experiments were carried out in an attempt to determine what component of this fixative was responsible for the reaction product observed. In addition, immersion fixation of tissue blocks was compared to the whole lung fixation method of Kikkawa ('70). Kikkawa ('70) achieved excellent preservation of the acellular alveolar lining layer by such a fixation technique.In all lungs examined, whenever a phosphate buffer was utilized with primary aldehyde fixation, an electron-dense precipitate was observed on the luminal surfaces of the type I and II pulmonary epithelial cells. Additional sites of reaction product were pinocytotic vesicles of the type I cells and membranous arrays within the alveolar lumen. Such deposits were never observed when a sodium cacodylate buffer was used. No such granules were observed in areas of lung where the acellular alveolar lining layer had been preserved.The implications of these findings with regard to lung histochemical procedures and the possible relationship of these phosphate buffer-dependent granules to the surfactant system are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091810302
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  • 4
    Electronic Resource
    Electronic Resource
    Electron microscopic observations on the morphogenesis of the albino rat lung, with special reference to pulmonary epithelial cellsSupported by research Fellowship grants 1-F1-GM-33, 009, 5-F1-GM-33, 009, and grant-in-aid GRSG-FR-05403 from the National Institutes of Health. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 127 (1970), S. 181-205 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Electron microscopic observations of developing albino rat lung provide further evidence for the endodermal origin of the type I and II pulmonary epithelial cells, and for the mesodermal origin of the interstitial pulmonary cells.Cytoplasmic glycogen increased in the fetal endodermal cells from day 16 to the twentieth day of gestation. Further development revealed a decrease of this substance in the differentiating pulmonary epithelial cells, and a concurrent increase in the mesodermal interstitial pulmonary cells, these being cells formerly characterized by their high lipid content. A continuous basement membrane delineated the mesodermal and endodermal components of the developing rat lung from day 16 to the third postnatal day. Tight junctions between adjacent endodermal epithelial cells were present throughout this same gestational period. Lamellar bodies, which are characteristic of the type II pulmonary epithelial cell, may be found simultaneously with large quantities of cytoplasmic glycogen. This feature is considered characteristic of fetal endodermal components.A definite continuum of ultrastructural changes may be traced from the endodermal, columnar epithelial cells to the definitive type I and II pulmonary epithelial cells. Comparable observations for the mesodermal components reveal a progression leading to mature interstitial pulmonary cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001270205
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