Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    An Improved Method to Obtain High Molecular Weight DNA from Purified Micro- and Macronuclei of Tetrahymena thermophila (1996)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 43 (1996), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An improved method to obtain high molecular weight DNA from purified macro- and micronuclei of Tetrahymena thermophila is described. Micro- and macronuclear DNA obtained using previously described protocols was degraded and not suitable for the cloning of large (〉 100 kb) DNA fragments. Based on the data reported here, we propose that DNA degradation is mainly due to nuclease activity; some micronuclear DNA degradation is due to mechanical shearing as a result of extended periods of blending. We have made modifications to reduce nuclease degradation by minimizing cell lysis, by the early addition of EDTA and by increasing the EDTA concentration (23 mM). To reduce mechanical shearing, cell and nuclear suspensions were blended for shorter periods. High molecular weight micro- and macronuclear DNA was obtained using the new protocol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1996.tb01391.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Evolution of Amitosis of the Ciliate Macronucleus: Gain of the Capacity to Divide (1991)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 38 (1991), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ciliates exhibit nuclear dimorphism, i.e. they have a germline micronucleus and a somatic macronucleus. Macronuclei are differentiated from mitotic sisters of micronuclei. The macronuclei of “higher ciliates” are polyploid and divide acentromerically (“amitotically”); they differentiate once per life cycle. By contrast, Karyorelict (KR) ciliate macronuclei are nearly diploid and cannot divide; they must differentiate at every cell cycle. Diverse lines of evidence are presented to support the hypothesis that ancestral ciliate macronuclei were incapable of division (as in living karyorelict ciliates) and that higher ciliates gained, perhaps independently more than once, the ability to divide the macronucleus. Selective pressures that could have driven the evolution and macronuclear division and two plausible step-wise pathways for the evolution of macronuclear division are proposed. These hypotheses are relevant to our understanding of amitosis mechanisms, evolution of nuclear dimorphism, and phylogenetic classification of ciliates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1991.tb04431.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The Genetic Control of Two Lethal Traits in Variety 1, Tetrahymena pyriformis (1960)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A breeding analysis of a wild strain (Indian Lake 12) of variety 1, Tetrahymena pyriformis, discloses recessive lethal alleles at two independently assorting loci. Conclusions concerning the cytogenetic behavior of this species have been verified through the scoring of a large number of offspring segregating the lethals. In particular, cytogamy does not occur in these strains with a frequency higher than 2%. if indeed it occurs at all. The finding of lethal heterozygosity in a wild strain suggests that close inbreeding usually does not take place in nature and supports the conclusion that variety 1 of T. pyriformis is an outbreeding species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1960.tb00710.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    PCR Amplification of Tetrahymena rDNA Segments Starting with Individual Cells (1991)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 38 (1991), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To facilitate studies of rDNA molecular genetics in Tetrahymena thermophila, we attempted the detection of polymorphisms in the nontranscribed spacers (NTSs) using polymerase chain reaction (PCR), starting with minute amounts of DNA. The targeted polymorphic regions are 85% adenine-thymine (AT). We found conditions of efficient and specific in vitro amplification of targeted segments in the replication domain of the 5′NTS and in the subtelomeric segment of the 3′NTS. The identity of the amplified segments was confirmed by restriction enzyme digestion and DNA sequence analysis. Digestion of the template DNA at restriction sites upstream and downstream of the targeted region increased the efficiency of amplification, presumably because the targeted segments are in a palindromic molecule. Starting from total cell DNA corresponding to as little as 0.03 picogram (equivalent to the DNA content of 0.003 cells or about 30 rDNA molecules), we observed the amplified band after agarose gel electrophoresis and ethidium bromide staining. The yield indicated more than 10-billion-fold amplification. Amplification of the subtelomeric fragment yielded homogeneous product of minimum possible length even though the telomeric-specific primer can bind, at least initially, at a multiplicity of GGGGTT repeats. Amplified 5′NTS product also was detected in an ethidium-bromide-stained gel when PCR was started with a single cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1991.tb01364.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Symposium on Ciliate Molecular Genetics: Introductory Remarks (1987)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 34 (1987), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1987.tb03203.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    The Tetrahymena thermophila genome sequence: 1. Genome-wide uniformity of macronuclear chromosome copy number in spite of amitotic (random) copy distribution (2005)
    Oxford, UK : Blackwell Science Inc
    The @journal of eukaryotic microbiology 52 (2005), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In Ciliates, the diploid, mitotic, genetically silent micronucleus is the cell's germline, while the macronucleus is the exclusive site of gene expression. Pre-genomic studies in Tetrahymena thermophila have shown that〈list xml:id="l2" style="custom"〉•The macronucleus contains 250–300 chromosomes, derived from the five germline chromosomes;•G2 copies of each chromosome are distributed amitotically (randomly) during macronuclear division.In heterozygotes, this results in the segregation of vegetative descendants expressing only one of the two alleles (assortment). Phenotypic assortment rate measurements for a handful of loci suggested a uniform macronuclear chromosome G1 copy number (∼45 per macronucleus; rDNA minichromosome excepted). The NIGMS/NSF-supported, whole-genome-shotgun sequencing of the T. thermophila macronuclear genome was recently completed at TIGR. (Sequence and scaffold databases, and related resources, are publicly available for blasting or downloading without restriction at ). Analysis of the depth of sequence coverage shows that (rDNA excepted) at least 95%—and possibly all—of the macronuclear genome is in chromosomes with uniform copy number. This implies the existence of regulatory mechanisms that independently correct the number of copies of nearly 300 different chromosomes after random distribution at every cell cycle.Supported by NIH grant RR02931. Preliminary sequence data were obtained from The Institute for Genomic Research website.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_1_65.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila (2002)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 49 (2002), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . To assess the utility of expressed sequence tag (EST) sequencing as a method of gene discovery in the ciliated protozoan Tetrahymena thermophila, we have sequenced either the 5’or 3’ends of 157 clones chosen at random from two cDNA libraries constructed from the mRNA of vegetatively growing cultures. Of 116 total non-redundant clones, 8.6% represented genes previously cloned in Tetrahymena. Fifty-two percent had significant identity to genes from other organisms represented in GenBank, of which 92% matched human proteins. Intriguing matches include an opioid-regulated protein, a glutamate-binding protein for an NMDA-receptor, and a stem-cell maintenance protein. Eleven-percent of the non-Tetrahymena specific matches were to genes present in humans and other mammals but not found in other model unicellular eukaryotes, including the completely sequenced Saccharomyces cerevisiae. Our data reinforce the fact that Tetrahymena is an excellent unicellular model system for studying many aspects of animal biology and is poised to become an important model system for genome-scale gene discovery and functional analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2002.tb00350.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Dual Capacity for Nutrient Uptake in Tetrahymena. II. Role of the Two Systems in Vitamin Uptake (1977)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Previously, we found that a mutant strain of Tetrahymena pyriformis without food vacuoles failed to grow unless the nutrient media were richly supplemented with vitamins and trace metals. Here we show that calcium folinate alone can replace the extra vitamin supplementation. The mutant requires ∼ 90-fold higher concentrations of folinate than the wild-type cells to give similar growth responses in a chemically defined medium. We infer that the food vacuole is an important route of uptake for this vitamin in the wild-type cells. We found no difference between mutant and wild-type cells in their requirements for nicotinic acid, pantothenic acid, riboflavin-monophosphate, and pyridoxal. We infer that an extravacuolar route contributes importantly to uptake of these 4 compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1977.tb01001.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    The Tetrahymena thermophila genome sequence. 2. Relating macronuclear sequence scaffolds to corresponding macronuclear chromosomes (2005)
    Oxford, UK : Blackwell Science Inc
    The @journal of eukaryotic microbiology 52 (2005), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tetrahymena's highly fragmented macronuclear genome contains ∼300 chromosomes that have recently been whole-genome-shotgun sequenced at TIGR. We had genetically mapped Random Amplified Polymorphic DNAs (RAPDs) to Tetrahymena's micro- and macronuclear genomes. We sized the MAC chromosomes that carry RAPDs by cloning and labeling RAPD DNA and probing Southern blots of macronuclear DNA separated by pulsed-field electrophoresis. We now report using cloned RAPD sequences to blastn-search the TIGR Tetrahymena scaffold database (); each RAPD sequence matched a sized scaffold. Comparison of physical sizes of MAC chromosomes (as determined by hybridization) to corresponding scaffold sizes showed that:〈list xml:id="l1" style="custom"〉•Every complete scaffold (assembled from telomere to telomere) matches in size with its corresponding MAC chromosome within 10% experimental error.•No incomplete scaffolds (telomere-capped at one or neither end) exceeded the size of its corresponding MAC chromosome.This work is allowing us to relate the genetic, physical and sequence maps of the micro-and macronuclear genomes to one another. It has also provided strong evidence for the quality and accuracy of the Tetrahymena genome sequencing and assembly carried out at TIGR. (Supported by NIH grant RR02931 and contract to TIGR NIGMS Tetrahymena genome sequencing grant. Sequence data were obtained from The Institute for Genomic Research website.)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_1_20.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Two adjacent genes encoding copper-induced-type metallothioneins in Tetrahymena thermophila may have resulted from a relatively recent gene duplication (2005)
    Oxford, UK : Blackwell Science Inc
    The @journal of eukaryotic microbiology 52 (2005), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tetrahymena thermophila is a valuable model organism for the study of metallothioneins, which play important roles in the metabolism of essential metal ions and in heavy-metal detoxification. Starting from a T. thermophila expressed sequence tag (EST) originally annotated as a Zn–Cu–Cd metallothionein (GenBank Acc #BM394105), we determined the genomic sequence corresponding to this EST and its flaking region. The sequence contains two adjacent genes encoding metallothioneins of the copper-induced type: the MTT2 gene (submitted to GenBank by Boldrin et al. as Cu-inducible while this work was in progress) and a second, novel gene (MTT4), located ∼1.4 kb downstream from and with same orientation as MTT2. Our entire sequenced segment is contained in the T. thermophila whole-genome sequence recently completed at TIGR. Both MTT4 and MTT2 are intronless and encode two nearly identical proteins (two differences out of 108 amino acids). The 5′ and 3′-flanking regions of both the metallothionein genes show significant sequence similarity as well, suggesting recent gene duplication. All glutamines use the CAA codon, reminiscent of codon usage observed in highly expressed Tetrahymena genes. MTT4 and MTT2 map to a 1.5-Mb macronuclear chromosome from the right arm of micronuclear chromosome 2.Supported by NIH grant RR02931. Sequence data were obtained from The Institute for Genomic Research Tetrahymena website,
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_1_6.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...