ISSN:
1432-0878
Keywords:
Hepatocytes
;
Cell culture
;
Papain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Differentiated cells have been established in monolayer culture from adult rat liver and their ultrastructural and biochemical features characterized after 20–30 generations. Hepatocytes were isolated by enzyme perfusion of the liver followed by treatment with papain, which allowed cultures to be established more readily and to be cloned at an early stage. Ultrastructural studies indicated that the cells were derived largely from hepatic parenchymal cells. The cells showed structural modifications during primary culture but were stable thereafter. The cultured cells retained some differentiated functions unique to liver cells, including the synthesis of ornithine from arginine and the secretion of serum proteins, albumin, α- and β-globulins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00214206
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