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  • 1
    Electronic Resource
    Electronic Resource
    Classical trajectory calculations of collision energy dependence of total and partial Penning ionization cross sections for He*(23S)+N2→He+N2++e− (1999)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 110 (1999), S. 3773-3780 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Classical trajectory calculations are performed for the Penning ionization system He*(23S)+N2→He(1 1S)+N2+(X˜ 2Σg+,A˜ 2Πu,B˜ 2Σu+)+e−. Anisotropic model potentials of He*(2 3S)+N2 are adapted to reproduce collision-energy dependence of ionic-state-resolved ionization cross sections observed by two-dimensional Penning ionization electron spectroscopy. Results of trajectory calculations are compared with those of ab initio potential surfaces obtained by Ishida. Opacity functions are demonstrated to be strongly dependent on ionic states, collision energy, and anisotropic varieties of trajectories. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.478268
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  • 2
    Electronic Resource
    Electronic Resource
    Collision-energy/electron-energy resolved two-dimensional study of Penning ionization of Ar by He metastable atoms 23S and 21S (1996)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 105 (1996), S. 7536-7542 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Selection of collision energies by a time-of-flight method has been combined with Penning ionization electron spectroscopy by utilizing a multichannel scaler and 2 MB random access memory. This technique provides a two-dimensional Penning ionization electron spectrum (2D-PIES) in which the Penning electron intensity is observed as a continuous function of both collision energies and electron energies. The observed 2D-PIES for Ar/He* (23S and 21S) was compared with calculated 2D-PIES obtained by classical trajectory calculations based on interaction potentials and transition probabilities. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.472579
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  • 3
    Electronic Resource
    Electronic Resource
    Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration (2001)
    Copenhagen : Munksgaard International Publishers
    Journal of periodontal research 36 (2001), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The interface between denuded dentin and regenerative periodontal tissue was investigated in a rat alveolar bone defect model using morphological and immuno-cytochemical approaches. The dentin surface was surgically exposed along the palatal roots of maxillary first molars. At 3 weeks post treatment, animals were perfused and treated regions from decalcified mandibles were embedded in Epon for ultrastructural studies or LR White for post-embedding immunogold labeling. Thin tissue sections were incubated with antibodies against noncollagenous matrix (osteopontin, bone sialoprotein, osteocalcin and fibronectin) and plasma (α2HS-glycoprotein and albumin) proteins. While in some cases, regenerative events took place directly on the denuded dentin surface, the interface between the denuded dentin and regenerating periodontal tissue was frequently characterized by the presence of an interfacial zone. This zone sometimes showed an electron-dense, cement line-like, planar accumulation of organic material immunoreactive for osteopontin and bone sialoprotein. Immunolabeling for osteocalcin and α2HS-glycoprotein was moderate and diffuse throughout the interfacial zone, whereas labeling with antibodies to albumin and fibronectin resulted in a weak reaction. It is concluded that accumulation of bone sialoprotein and osteopontin is a primary event during the formation of regenerative cementum onto denuded root surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0765.2001.036004205.x
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of endothelins and their receptors in cells from human periodontal tissues (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 38 (2003), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective:  The present study investigated the presence of ET-1 in gingival crevicular fluid (GCF) from patients with periodontitis, and the expression of endothelins (ETs) and their receptors mRNA in cultured cells from human periodontal tissues.Background:  ET was originally discovered as a potent vasoconstrictive peptide from endothelial cells. It has been reported that ETs are produced by various cells besides endothelial cells. ETs are related to inflammatory and sclerotic lesions, such as arteriolosclerosis and hepatic cirrhosis. Therefore, ETs may be involved in periodontal disease. However, the roles of ETs in development and progression of periodontal disease are not clear.Methods:  ET-1 released from the cultured cells was measured by enzyme-linked immunosorbent assay. mRNA expressions for ETs and their receptors were examined by reverse transcription-polymerase chain reaction and Northern blotting analysis.Results:  ET-1 levels in GCF from patients with periodontitis were higher than those from healthy subjects. Human gingival keratinocytes (HGK) expressed mRNA for ETs and their receptors, ET-Ar and ET-Br. ET-1 mRNA expression and ET-1 peptide production from HGK were enhanced by interleukin-1β and tumor necrosis factor-α.Conclusions:  These results suggest that ET-1 plays a significant role in periodontal disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0765.2003.00653.x
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  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair (2003)
    Oxford, UK : Munksgaard International Publishers
    Journal of periodontal research 38 (2003), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To clarify the roles of epithelial cell rests of Malassez (ECRM) during periodontal repair, experimental root resorption was induced in rats and then the ECRM that existed in periodontal ligament during cementum repair was investigated using morphological and immunohistochemical approaches. At day 7, after mechanical injury, root resorption was observed and ECRM were present adjacent to the site of resorption lacunae. They were observed in periodontal ligament adjacent to site of the resorption lacunae. These ECRM were immunoreactive for bone morphogenetic protein-2. During the stage of early cementum repair, the ECRM were immunoreactive for osteopontin and ameloblastin. They strongly reacted to proliferating cell nuclear antigen. In uninjured control sections, ECRM located in the periodontal ligament adjacent to cementum were not immunoreactive for any antibodies. These findings suggested that ECRM may be related to cementum repair by activating their potential to secrete matrix proteins which have been expressed in tooth development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0765.2003.01636.x
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural and ultracytochemical characteristics of multinucleated cells after hydroxyapatite implantation into rat periodontal tissue (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 27 (1992), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Multinucleated cells (MNCs) that appeared after hydroxyapatite (HAP) implantation into experimentally-produced bone defects in rat periodontal tissues were investigated both ultrastructurally and ultracytochemically. At day 5 after implantation, MNCs first appeared along the HAP surface. They had no features of typical osteoclasts such as ruffled border and clear zone. By d 14, these cells acquired features similar to osteoclasts, including ruffled border and clear zone. With the appearance of ruffled borders in MNCs, new bone deposited around the implanted HAP. MNCs appeared to excavate both newly-formed bone and implanted HAP simultaneously. Ingested HAP particles were observed not only in MNCs but also in macrophages. MNCs contained both tartrate-resistant acid phosphatase (ACPase) and carbonic anhydrase (CAase). ACPase activity was detected along all the biosynthesizing pathways in MNCs. Extracellular ACPase activity around the ruffled border region was also demonstrable. CAase activity could be detected only in the cytosol, vesicles and mitochondorial cristae of the MNCs. These cytochemical characteristics were almost the same regardless of the time elapsed after implantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1992.tb02085.x
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of serum fractions on single-cell mouse embryos in vitro (1987)
    Springer
    Journal of assisted reproduction and genetics 4 (1987), S. 153-158 
    Details
    ISSN: 1573-7330
    Keywords: serum ; fractions ; effects ; in vitro fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To examine the effect of various fractions of human fetal cord serum (HCS) on mouse embryos cultured in vitro, heat-inactivated HCS was separated by ultrafiltration into five distinct fractions: Fractions A, MW〉30,000; B, MW 30,000−10,000; C, MW 10,000−5000; D, MW 5000−1000; and E, MW 〈1000. Seven hundred twentyeight single-cell embryos were cultured in TYH- 280 medium supplemented with 8 mg/ml bovine serum albumin (BSA) and a 20% concentration of Fraction A, B, C, D, or E, whole HCS, or BSA alone. Embryos cultured with Fraction A or E or whole HCS demonstrated a significantly reduced growth rate (P〈0.01), while embryos cultured with Fraction D demonstrated a significantly increased growth rate (P〈0.01). Additionally, 649 singlecell embryos were cultured in medium which was supplemented with 8 mg BSA/ml and a 0, 1,2, or 5% concentration of Fraction A or E. Fraction E displayed toxicity even at a 1% concentration (P〈 0.07), while Fraction A demonstrated growth inhibition at a 5% concentration (P 〈0.05) but increased the hatching rate at a 1% concentration (P 〈 0.01). Finally, 635 single-cell embryos were cultured with four distinct fractions of HCS obtained from a Sephacryl S-200 column: Fractions I, MW 100,000; II, MW 70,000−100,000; III, MW 30,000−70,000; and IV, low molecular weight (〈5000). Fraction I or III significantly reduced the embryo growth rate as seen with Fraction A (P〈0.01) and Fraction II significantly increased only the hatching rate (P〈0.01), while Fraction IV significantly increased the growth rate as seen with Fraction D. In conclusion, HCS contains embryo growth inhibitory properties in the high (〉30,000) and low (〈1000) molecular weight components, while growth promoting factors are found in the 1000−5000 MW fraction. It also seems that there are some factors in the 70,000−100,000 MW fraction which may promote the ability of the embryo to hatch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01555462
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