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  • 1
    Electronic Resource
    Electronic Resource
    Factor which affects the mode of genetic recombination in E. coli (1975)
    [s.l.] : Nature Publishing Group
    Nature 253 (1975), S. 138-140 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The transfer of genetic material can result in the formation of two possible types of recombinant clones: (a) substitution type resulting from replacement of recipient genetic material by a homologous segment of donor material, and (b) addition type resulting from addition of the donor genetic ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/253138a0
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  • 2
    Electronic Resource
    Electronic Resource
    Specialised transformation in Escherichia coli K12 (1974)
    [s.l.] : Nature Publishing Group
    Nature 248 (1974), S. 112-116 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A specialised transformation system has been developed in E. coli K12 by using specialised transducing phage DNA as donor DNA. Transformation of a marker on a F′ episome has also been ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/248112a0
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  • 3
    Electronic Resource
    Electronic Resource
    Synchronous and Dichotomous Replications of the Bacillus subtilis Chromosome During Spore Germination (1964)
    [s.l.] : Nature Publishing Group
    Nature 204 (1964), S. 1069-1073 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] GERMINATION of bacterial spores provides can unusual opportunity for analysing the process of development of a metabolicaly active STATE FROM AN INERT OR DORMANT STATE1. The mode of chromosome replication during spore germination, therefore, should be of partcular interest because of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2041069a0
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  • 4
    Electronic Resource
    Electronic Resource
    Chromosomal localization of Sau3A repetitive DNA revealed by in situ hybridization (1988)
    Springer
    Chromosoma 96 (1988), S. 372-375 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The Sau3A DNA family consists of unique alphoid human repetitive DNA which is prone to be excised from the chromosomes and exhibits restriction fragment length polymorphism. We studied the chromosomal localization of the DNA by in situ hybridization using cultured normal human lymphocytes. Under standard hybridization conditions, the sequence hybridized with the centromeric regions of chromosomes 1, 2, 4, 11, 15, 17, 18, 19 and X, but under high stringency hybridization conditions, it hybridized with the centromeric regions of chromosomes 1, 17 and X, and particularly chromosome 11. Based on these results, we discuss the evolutionary relationship among the sequences of the Sau3A DNA family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330704
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  • 5
    Electronic Resource
    Electronic Resource
    A restriction fragment length polymorphism (RFLP) locus of rat (Rattus norvegicus) linked to theCs-1 locus of rat linkage group XIII (1992)
    Springer
    Biochemical genetics 30 (1992), S. 537-544 
    Details
    ISSN: 1573-4927
    Keywords: restriction fragment length polymorphisms ; rat ; catalase locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A novel restriction fragment length polymorphism (RFLP) in inbred rats was revealed by Southern blot analysis with a clone arbitrarily chosen from a rat genomic library as a probe. A clone named α403 showed interstrain variations in the length of theEcoRI andHindIII fragments. TheEcoRI fragments were either 0.7 or 3 kb, those ofHindIII were either 4.5 or 5 kb, and three types were identified as combinations of those fragments in 20 inbred rat strains. These types segregated in backcross progeny as codominant alleles. The locus for the RFLP was thus namedA403. Analysis of linkage between the RFLP locus and 13 other loci reveal that theA403 locus was closely linked to theCs-1 locus (15 ± 5.2%), which belongs to rat linkage group XIII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01037592
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic properties of arsenate sensitive mutants of Bacillus subtilis 168 (1972)
    Springer
    Molecular genetics and genomics 118 (1972), S. 295-310 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Arsenate sensitive mutants were isolated from Bacillus subtilis strain 168 after treatment with N-methyl-N′-nitro-N-nitrosoguanidine or ethyl methane sulfonate. Though all mutants are phenotypically identical, a high proportion (40%) of the induced mutations are of a multisite nature as they do not revert spontaneously and are poorly transformable to arsenate resistance with wild type DNA. On the basis of transformation efficiency, UV inactivation kinetics and cotransduction frequency of outside markers, four independently isolated multisite arsenate sensitive mutations are characterized as resulting from large deletions of homogenous size (24000±6000 base pairs). The arsenate resistance locus was mapped between phe and aroD on the B. subtilis chromosome by PBS1 mediated transduction. Mechanisms for the formation of such chromosomal deletions are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333565
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  • 7
    Electronic Resource
    Electronic Resource
    The nature of the transformation process in Escherichia coli K12 (1973)
    Springer
    Molecular genetics and genomics 124 (1973), S. 1-10 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nature of the transformation process in E. coli was studied by investigating various factors which affect the efficiency of transformation. CaCl2 treatment of the recipient cells is absolutely necessary for transformation and the optimum concentration was found to be 30 mM. The efficiency of transformation is dependent upon temperature during incubation of the recipient cells with DNA. The efficiency is also affected by the molecular weight of donor DNA used. Sheared DNA with molecular weights ranging from 10 to 30x106 daltons was most efficient, increasing the number of transformants by a factor of 5 to 10 as compared to unsheared DNA. The intracellular status of recB-recC DNase (ATP-dependent DNase) is another important factor which determines the transformability of E. coli K12. This was shown by demonstrating that a recB - recC - sbcA - strain was transformable as well as the previously demonstrated recB - recC - sbcB - strain. Therefore, it seems reasonable to conclude that the E. coli K12 strain is transformable if the ATP-dependent DNase is absent or diminished in function and a state of recombinational proficiency exists.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267159
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  • 8
    Electronic Resource
    Electronic Resource
    The molecular mechanism of virus induction (1976)
    Springer
    Molecular genetics and genomics 148 (1976), S. 131-138 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The basic conditions for use of a new biochemical assay of bacteriophage induction are described. This method is based on the read-through transcription of the tryptophan operon integrated into an “early” transcribed region of the bacteriophage ϕ80 or λ genome. Inactivation of repressor molecules was assayed by measuring, in the presence of tryptophan, anthranilate synthetase activity in an Escherichia coli (trpE −) ϕ80 or λ lysogen infected with ϕ80ptrp or λptrp, respectively, and treated with mitomycin C or UV irradiation. This method provides a sensitive and easy means to analyze the induction process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268376
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  • 9
    Electronic Resource
    Electronic Resource
    Stimulation of DNA-mediated transformation by UV irradiation of recipient (mouse FM3A) cells (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 453-457 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: DNA-mediated transformation for thymidine autotrophs (Tk+) was stimulated severalfold when the recipient (mouse FM3A) (Tk-) cells were preirradiated by UV light. The effect was most prominently seen with the cells irradiated 12-16 hours prior to DNA addition. A similar stimulatory effect was observed when the cells were treated with novobiocin or mitomycin C. The effect, however, was blocked when cycloheximide was present during postirradiation period, suggesting that de novo protein synthesis is required for producing the effect. Most of the Tk+ transformants arised from the UV-irradiated cells carried the donor DNA sequences in their chromosomes.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041210302
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  • 10
    Electronic Resource
    Electronic Resource
    Differential cloning using in-gel competitive reassociation (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 286-290 
    Details
    ISSN: 0173-0835
    Keywords: Mammalian genome ; Rearrangement ; Polymorphism ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We describe the principle and actual processes of a differential cloning procedure designed for cloning of anonymous restriction DNA fragments whose molecular sizes differ between two genomic DNA preparations from higher organisms as a result of DNA rearrangement, polymorphism, etc. The procedure, which was extensively modified from the original one and still employs in-gel competitive reassociation (IGCR) as the basic principle, aims for cloning of DNA fragments which exist in one copy or less per mammalian genome. The modified procedure consists of dissociation and reassociation of biotinylated restriction digests of target DNA fragments (from which clones are to be isolated) in the presence of a large excess of reference (competitor) DNA in gel after electrophoresis, which is followed by absorption of the target DNA fragments to streptavidin-coated tubes and solid-phase polymerase chain reaction. After repeating these steps we attained substantial enrichment of altered DNA fragments which were originally present in one copy or less per complex mammalian genome.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150160145
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