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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of Viosamine (4-Amino-4,6-dideoxy-D-glucose) by Double Inversion at C-4 and Identification with the 4-Amino-4,6-dideoxyhexose from Escherichia coli Strain B (1964)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 86 (1964), S. 2939-2940 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01068a038
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  • 2
    Electronic Resource
    Electronic Resource
    A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome (1996)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: There is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB–mutL operons at 94.7 min on the genetic map. Affinity of DnaA protein for DNA was measured in vivo as the activity of β-galactosidase produced from the lacZ gene on a plasmid fused to the 5′-terminal portion of the mioC gene, which is under the control of the DnaA protein. The chromosomal DNA segment between the two operons, carried on a compatible plasmid, derepressed the β-galactosidase activity by titrating DnaA protein. Derepression occurred on the chromosomal dnaA gene as well, since it is autoregulated. Hence, as measured by immunoassays, one plasmid molecule carrying the DnaA-binding region titrated 370 DnaA molecules, which is a value eightfold higher than that for a plasmid containing the oriC region. We estimate that about 60% of the total cellular DnaA molecules are bound to this site. Four DnaA-binding sequences (DnaA boxes) and a DnaA-regulated promoter directing transcription of two small genes were present within a 250 bp stretch in this region but additional long DNA regions, including the fifth DnaA box located about 650 bp downstream, were required for maximum binding. A role for the DnaA-binding site in controlling DnaA-protein concentration in the cell cycle is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.453983.x
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of YAC–based mammalian artificial chromosomes (1998)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 16 (1998), S. 431-439 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0598-431
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  • 4
    Electronic Resource
    Electronic Resource
    DNA Chain Growth: In Vivo and in Vitro Synthesis in a DNA Polymerase-negative Mutant of E. coli (1970)
    [s.l.] : Nature Publishing Group
    Nature 228 (1970), S. 223-226 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The enzyme involved in the discontinuous replication of DNA seems not to be the Kornberg polymerase but to be associated with the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/228223a0
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  • 5
    Electronic Resource
    Electronic Resource
    Assay of centromere function using a human artificial chromosome (1998)
    Springer
    Chromosoma 107 (1998), S. 406-416 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In order to define a functional human centromere sequence, an artificial chromosome was constructed as a reproducible DNA molecule. Mammalian telomere repeats and a selectable marker were introduced into yeast artificial chromosomes (YACs) containing alphoid DNA from the centromere region of human chromosome 21 in a recombination-deficient yeast host. When these modified YACs were introduced into cultured human cells, a YAC with the alphoid DNA from the α21-I locus, containing CENP-B boxes at a high frequency and a regular repeat array, efficiently formed minichromosomes that were maintained stably in the absence of selection and bound CENP-A, CENP-B, CENP-C and CENP-E. The minichromosomes, 1–5 Mb in size and composed of multimers of the introduced YAC DNA, aligned at metaphase plates and segregated to opposite poles correctly in anaphase. Extensive cytological analyses strongly suggested that the minichromosomes had not acquired host sequences and were formed in all cases by a de novo mechanism. In contrast, minichromosomes were never produced with a modified YAC containing alphoid DNA from the α21-II locus, which contains no CENP-B boxes and has a less regular sequence arrangement. We conclude that α21-I alphoid DNA can induce de novo assembly of active centromere/kinetochore structures on minichromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050324
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  • 6
    Electronic Resource
    Electronic Resource
    RNA-linked nascent DNA pieces in T7 phage-infectedEscherichia coli cells (1977)
    Springer
    Molecular genetics and genomics 154 (1977), S. 263-267 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The presence of RNA-linked nascent DNA pieces in T7 phage-infectedEscherichia coli cells has been shown by the selective degradation of the 5′-hydroxyl-terminated nascent DNA, produced by alkali or RNase treatment, with spleen exonuclease. At 43°C, the proportion of RNA-linked DNA pieces in nascent short DNA is 50 to 60% in T7ts136 (ts mutant of gene 6) phage-infectedE. coli, whereas that in T7 wild-type phage-infected cells is less than 6%. Joining of the nascent pieces is greatly retarded in T7ts136-infectedE. coli temperature sensitivepolA mutants at 43° C. These results suggest that gene 6 exonuclease plays a role in removal of the linked RNA during the discontinuous replication of T7 DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571281
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  • 7
    Electronic Resource
    Electronic Resource
    Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli (1984)
    Springer
    Molecular genetics and genomics 193 (1984), S. 231-237 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC)·poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-type cells). This mutant formed small colonies at 43 °C. At this temperature, accumulation of nascent fragments was more prominent in the rnh-91·polA4113 double mutant than in the polA4113 mutant; however, no accumulation was found in the rnh single mutant at 43° C. Unlike the 1–3 nucleotide primer RNA found on nascent fragments of polA4113 cells, primers from the rnh-91·polA4113 cells ranged from one to about ten bases. These results suggest that the 5′→3′ exonuclease activity of DNA polymerase I plays a major role in removal of primer RNA and that RNase H functions in an auxiliary role, excising the 5′-portion of longer primers. The rnh mutant supports replication of ColE1-type plasmids. A possible mechanism of replication of such plasmids in rnh mutants and a role of RNase H in the initiation of chromosomal replication are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330673
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  • 8
    Electronic Resource
    Electronic Resource
    Initiation site of deoxyribonucleotide polymerization at the replication origin of the Escherichia coli chromosome (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 422-431 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new round of chromosomal replication of a temperature-sensitive initiation mutant (dnaC) of Escherichia coli was initiated synchronously by a temperature shift from a nonpermissive to a permissive condition in the presence of arabinosyl cytosine. Increased amounts of nascent DNA fragments with homology for the chromosomal segment containing the replication origin (oriC) were found. The nascent DNA fragments were purified and treated with alkali to hydrolyze putative primer RNA and to expose 5′-hydroxyl DNA ends at the RNA-DNA junctions: The ends were then labeled selectively with T4 polynucleotide kinase and [γ-32P]ATP at 0°C and the terminally-labeled initiation fragments were purified by hybridization with origin probe DNAs containing one each of the constituent strands of oriC-DNA segment. The 32P-labeled initiation sites were then located at the resolution of single nucleotides in the nucleotide sequence of the oriC segment after cleavage with restriction enzymes. Two initiation sites of DNA synthesis, 37 nucleotides apart, were detected in one of the component strands of the oriC; in other words, in the strand whose 5′ to 3′ polynucleotide polarity lies counterclockwise on the E. coli genetic map. The results support the involvement of the primer RNA in the initiation of DNA synthesis at the origin of the E. coli genome and suggest that the first initiation event is asymmetric.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325904
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  • 9
    Electronic Resource
    Electronic Resource
    Change in priming sites for discontinuous DNA synthesis between the monomeric and concatemeric stages of phage T7 replication (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 400-406 
    Details
    ISSN: 1617-4623
    Keywords: Bacteriophage T7 ; Monomer replication ; Concatemer replication ; Primer RNA ; Semidiscontinous replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have analyzed the transition sites between primer RNA and DNA in a 589 bp segment of the bacteriophage T7 genome. In the monomeric replication stage, RNA-DNA transition sites are predominantly on the light (L) strand (with, 5′→3′ polarity on the genetic map) but rarely on the heavy (H) strand, indicating that replication proceeds semidiscontinuously with the H and L strands corresponding to the leading and lagging strands, respectively. The direction of replication is that expected from the position of the primary origin and also indicates that secondary origins are seldom if ever used. In the concatemeric stage of replication, RNA-DNA transition sites are instead distributed on both strands of the segment with equally high frequency, showing that initiation occurs within the concatemeric molecule per se and by a different mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425692
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  • 10
    Electronic Resource
    Electronic Resource
    Site-specific base deletions in human α-satellite monomer DNAs are associated with regularly distributed CENP-B boxes (1997)
    Springer
    Chromosome research 5 (1997), S. 207-211 
    Details
    ISSN: 1573-6849
    Keywords: α-satellite DNA ; CENP-B ; CENP-B box ; primer ; direct sequencing ; 2-mer repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018407316908
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