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  • 1
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: White spot syndrome virus (WSSV) has been a major pathogen of cultured Penaeus monodon Fabricius in Malaysia since 1994. As quantitative study on the replication of WSSV is in its infancy, competitive polymerase chain reaction (PCR) was used for quantitative study of an experimental WSSV infection per os in growout P. monodon. Gills, abdominal integument and abdominal muscle were selected for viral quantification. Infection was detectable as early as 14 h postinfection (h p.i.) in both gills and integument, but the infection in muscle was only detected at 24 h p.i. Gill tissue had the highest viral load, followed by integument and muscle. Typical viral growth curves were obtained for all organs with distinct phases of eclipse (0–24 h p.i.), logarithmic (24–48 h p.i.) and the plateau (48–120 h p.i.). Cumulative mortality rapidly increased from 48 h p.i. and reached 100% at the end of the plateau phase at 120 h p.i. Gross signs of white spots and reddish discoloration were also obvious in moribund individuals from the plateau phase. Based on the three phases of viral growth, WSSV infection was classified into light, moderate and heavy infection stages.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 208 (1965), S. 307-308 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To assess its pathogenicity for cattle, 8 colostrum-deprived calves were inoculated intranasally on the day of birth with 10 ml. of BK culture fluid containing WBR 1 strain and with a further 10 ml. intratracheally 24 h later. The inocula had a mean titre of 104- TCID5Q per ml. The calves were kept ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Veterinary research communications 8 (1984), S. 55-59 
    ISSN: 1573-7446
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One of 13 healthy dogs used in a pharmacokinetic study of imidocarb dipropionate died due to difficulty in breathing, tachycardia, weakness and profuse diarrhoea. Autopsy findings showed marked pulmonary congestion and oedema. Kidneys were grossly enlarged and markedly congested with extensive haemorrhage in the cortex and medulla. Marked tubulonephrosis was also exhibited microscopically. Liver and spleen were moderately enlarged and congested. The adverse effects of imidocarb may be due to excessive acetylcholine action.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-7446
    Keywords: concurrent disease ; goats ; haemonchosis ; pasteurellosis ; stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Tropical animal health and production 14 (1982), S. 177-184 
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Deux essais effectués sur 2000 poulets de chair ont montré que ces poulets vaccinés avec du vaccin lyophilisé “F” soit à leur naissance, au 21 et au 42e jours ont été protégés à leur 14, 35, 56 et 70e jours lors d'épreuves infestantes par du virus de la maladie de Newcastle administré par voies intramusculaire, intranasale ou par contact. Un pourcentage élevé des oiseaux vaccinés a été protégé en dépit d'un niveau d'anticorps hemagglutinants constamment bas. Les autopsies pratiquées sur des animaux témoins et sur les animaux vaccinés morts à la suite d'épreuves infestantes ont montré que la plupart d'entre eux présentaient des lésions intestinales.
    Abstract: Resumen Se realizaron dos ensayos con 2,000 pollos de engorde los cuales se vacunaron con la vacuna F liofilizada contra Newcastle. Las vacunaciones se hicieron en forma seriada los dias 1, 21, y 42, protegiendo a las aves contra la descarga de virus virulento de Newcastle efectuada los días 14, 35, 56 y 70, administrada por vía intramuscular, intranasal y por contacto. Un alto porcentaje de los pollos fue protegido por la vacuna, aunque los títulos de anticuerpos IH fueron bajos. Las necropsias de las aves muertas durante los ensayos mostraron lesiones intestinales.
    Notes: Summary Two field trials carried out on 2,000 broiler chickens showed that chickens vaccinated with the lyophilised Newcastle disease (ND) “F” vaccine at 1, 21, and 42 days were protected when challenged at 14, 35, 56 and 70 days with the virulent strain of Newcastle disease virus (NDV) administered intramuscularly, intranasally, or by contact. A high percentage of the vaccinated birds were protected even though the haemaggglutination inhibition (HI) antibody levels were persistently low. Autopsies of the control and vaccinated birds that died from the challenge showed that most of the birds had lesions in the intestines.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-0778
    Keywords: baculovirus ; cloning ; haemagglutinin-neuraminidase ; Newcastle disease virus ; recombinant protein expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The haemagglutinin-neuraminidase (HN) gene ofNewcastle disease virus (NDV) strain AF2240, amplifiedfrom the viral genomic RNA (∼1.8 kb) was directionallycloned and inserted into a baculovirus expressionvector system. The recombinant glycoprotein expressedin Spodoptera frugiperda (Sf9) cellsshowed haemagglutinin (HA), neuraminidase (NA) andhemadsorption activities. HA activity was detected inboth extra- and intra-cellular recombinant HN(recHNAF2240) samples. In addition, both HA andhemadsorption activities were inhibited by polyclonalanti-NDV sera. Furthermore, significant expression ofthe recombinant protein was observed on the surface ofinfected cells. SDS-PAGE analysis revealed thepresence of visually distinguishable bands between the70 and 80 kDa in size that were absent in thewild-type samples. Western blot analysis showed thatthe distinct ∼63 kDa band and a ∼75 kDa bandcorresponded to the unglycosylated and glycosylated HNglycoprotein respectively as reported in anotherstudy. These observations indicated that the HNrecombinant protein was not only expressed on thesurface of the infected cells as well as with theviral coat protein, but also appears to be functional.
    Type of Medium: Electronic Resource
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