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  • 1
    Electronic Resource
    Electronic Resource
    Clinicopathological characteristics of tongue piercing: an experimental study (2004)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 33 (2004), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  This study attempts to remedy the situation that no morphological studies exit of tongue piercing.Methods:  The tongues of eight Beagles were pierced with titanium implants. The animals were then divided into four groups: 1 week, 2 weeks, 1 month and 3 months for clinical-photographic and histopathological studies.Results:  Group I: clinically, erythema at both orifices and, microscopically, granulation tissue covering the surface of the canal and infiltrates of polynuclear neutrophils. Group II: persistence of erythema and of the granulation tissue, with the edges of the epithelium showing signs of regeneration. Group III: granulation tissue alternating with fibrosis; epithelium covering the sides of the canal. Group IV: fibrosis substituting the granulation tissue and almost complete re-epithelialization of the canal. All groups, except group I, numerous foreign body-type granulomas.Conclusions:  Tongue piercing provokes fibrous reparation with almost total re-epithelialization of the perforated zone and foreign body-like granulomatous inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.2004.00219.x
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  • 2
    Electronic Resource
    Electronic Resource
    Azelaic Acid Has Sensitizing Effect in the Chemotherapeutic Treatment of Several Melanoma Cell Lines (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Periodontology 2000 9 (1996), S. 0 
    Details
    ISSN: 1600-0757
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Chemotherapy for melanoma results in low response and must be reinforced with sensitizer compounds. We believed that azelaic acid (AZA) could modulate melanomas’resistance to antineoplastics. Therefore we tried to compare in vitro treatment with antineoplastics alone versus AZA treatment followed by antineoplastics. We carried out MTT assays to evaluate the cytotoxicity of melphalan, lomustine (CCNU), fotemustine, and 4-Hydroxyanisole (4-HA) on three melanoma lines (B16F10, SK-MEL-28, and SK-MEL-1), and the modulating effect of pretreatment with AZA (1 mM). AZA showed a dose-dependent antineoplastic activity on the three lines. Melphalan was the most active drug followed by CCNU, fotemustine, and 4-HA. The most sensitive line was B16F10 and the least sensitive was SK-MEL-1. Previous treatment with AZA of B16F10 reinforced the effect of melphalan (2.5 times), CCNU (10 times), and fotemustine (14 times); whereas for SK-MEL-28 and SK-MEL-1, only the cytotoxicity of CCNU and fotemustine increased. An antagonist effect was produced by 4-HA on all three lines. We concluded that AZA enhances in vitro cytotoxicity of CCNU and fotemustine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0749.1996.tb00124.x
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  • 3
    Electronic Resource
    Electronic Resource
    DAMAGE IN B2m GENES AND DNA METHYLATION OF H-2 GENES ARE INVOLVED IN LOSS OF EXPRESSION OF CLASS I MHC PRODUCTS ON THE MEMBRANE OF LR.4, A CELL LINE DERIVATIVE OF THE T-CELL LYMPHOMA L5178Y (1992)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 19 (1992), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have isolated an H-2 deficient cell line (LR.4) from the T-cell lymphoma L5178Y which grew without restrictions in the peritoneal cavity of different inbred strains of mice. The use of polyclonal anti-H-2 antiserum and complement indicated that LR.4 cells did not express class I determinants on the cell membrane. Southern blots of genomic DNA of LR.4 cells showed that B2m genes were severely damaged and that class I H-2 genes were extensively methylated. Consequently, LR.4 cells failed to transcribe mRNAs for both B2m and class I H-2 genes. On the other hand, specific immunity to LR.4 was demonstrated in C57BL/6J mice since, in subsequent challenges with either LR.4 or EL4.4, LR.4 did not grow, whereas EL4.4 grew and killed the mice. In C57BL/6J mice, rejection of LR.4 was accompanied by the production of cytotoxic antibodies. The immune response induced in C57BL/6J mice was determined by non-H-2 antigenic determinants in LR.4 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1992.tb00053.x
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of fracture resistance of endodontically treated maxillary premolars, restored with ceromer or heat-pressed ceramic inlays and fixed with dual-resin cements (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 31 (2004), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  The aim of this study was to evaluate the fracture resistance of endodontically maxillary premolars restored with mesio-occlusal-distal (MOD) inlays made with ceramic (IPS-Empress 2) and ceromer (Targis) and luted with three different dual-cured resin cements (Enforce, Variolink II, Panavia F). Sixty maxillary premolars were randomly distributed into six groups, according to their mesio-distal and facio-lingual dimensions. The teeth were endodontically treated and MOD cavities prepared. After the restorations were cemented, the samples were thermocycled and submitted to an axial compressive load by the action of a rounded end steel cylinder contacting the incline planes of occlusal surfaces of the teeth. The mode of fracture was analysed with a microscope. The best results were found with the combinations (cement/restorative material) Enforce/Targis (107·57 kgf) and Enforce/Empress (90·21 kgf) followed by Variolink II/Targis (86·44 kgf)–Variolink II/Empress (84·07 kgf) and Panavia F/Targis (82·43 kgf)–Panavia F/Empress (76·73 kgf). Analysis of variance (P 〈 0·05) showed a significant difference between Enforce and Panavia cements regardless of the restorative material. Considering the same luting agent there was no statistically significant difference between the restorative materials. Fracture of lingual cusps occurred in 55 of the 60 teeth and most of them were of the cohesive type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2003.01239.x
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  • 5
    Electronic Resource
    Electronic Resource
    Dermatomycose experimental, étude clinico-pathologique (1981)
    Springer
    Mycopathologia 76 (1981), S. 133-138 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Abstract Using rabbits, we have studied the effect of reinfection of T. mentagrophytes var granulosum and T. rubrum into lesions that were previously infected and resolved. Clinical mycological and histopathological studies were done for 16 weeks. Timentagrophytes produced a more severe infection than T. rubrum. The clinical lesions produced by reinoculation were less intense and long lasting.
    Notes: Résumé On réalise une étude expérimentale sur des lapins inoculés et réinoculés par Trichophyton mentagrophytes var. granulosum et Trichophyton rubrum, pour connaitre l'évolution clinique, mycologique et histopathologique pendant 16 semaines. Au cours de la premiere inoculation, les lésions cliniques, ainsi que le patron histopathologique produit par T. mentagrophytes var. granulosum, furent beaucoup plus grandes en intensité et durée que celles dûes á T. rubrum. Au cours de la réinoculation, les lésions cliniques produites para les deux dermatophytes ont eu une intensité et une durée moindres.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437193
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  • 6
    Electronic Resource
    Electronic Resource
    Organization of ATPA coding and 3′ flanking sequences associated with cytoplasmic male sterility in Phaseolus vulgaris L. (1992)
    Springer
    Current genetics 22 (1992), S. 147-153 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial F1 ATPase subunit A gene ; Cytoplasmic male sterility ; Phaseolus vulgaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A region of the mitochondrial genome associated with cytoplasmic male sterility (CMS) in Phaseolus vulgaris was flanked by two different repeated sequences designated x and y. The DNA sequence of the CMS-unique region and a portion of each flanking repeat was determined. Repeat x contained a complete coding copy of the F1 ATPase subunit A (atp A) gene, as well as an open reading frame (orf) predicting a protein of 209 amino acids. The TGA termination codon of the atpA gene and the ATG initiation codon of orf209 were overlapping. These reading frames were oriented with their 3′ ends proximal to the CMS-unique region. The CMS-unique region of 3736 nucleotides contained numerous orfs. The longest of these predicted proteins being of 239, 98 and 97 amino acids. The 3′ coding and 3′ flanking regions of orf98 were derived from an internal region of the higher plant chloroplast tRNA alanine intron. The region of repeat y immediately adjacent to the CMS-unique region contained the 111 carboxy-terminal coding residues of the apocytochrome b (cob) gene. This segment was oriented with its 5′ end proximal to the CMS-unique region, but cob gene sequences were not fused to an initiation codon within the unique region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351475
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  • 7
    Electronic Resource
    Electronic Resource
    DNA restriction fragment length polymorphisms correlate with isozyme diversity in Phaseolus vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 806-811 
    Details
    ISSN: 1432-2242
    Keywords: Common bean ; Molecular markers ; Phaseolin ; Phaseolus coccineus ; Gene pools
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic variation in Phaseolus vulgaris L. (P. vulgaris) was investigated at the isozyme and DNA levels. We constructed a library of size-selected Pst I clones of P. vulgaris nuclear DNA. Clones from this library were used to examine 14 P. vulgaris accessions for restriction fragment length polymorphisms (RFLPs). DNAs from each accession were analyzed with three restriction enzymes and 18 single copy probes. The same accessions were also examined for variability at 16 isozyme loci. Accessions included four representatives of the T phaseolin group and five representatives each of the C and S phaseolin groups. One member of the S group (the breeding line XR-235-1-1) was derived from a cross between P. vulgaris and P. coccineus. Isozymes and RFLPs revealed very similar patterns of genetic variation. Little variation was observed among accessions with C and T phaseolin types or among those with the S phaseolin type. However, both isozyme and RFLP data grouped accessions with S phaseolin separately from those accessions with C or T phaseolin. The highest degree of polymorphism was observed between XR-235-1-1 and members of the C/T group. RFLP markers will supplement isozymes, increasing the number of polymorphic loci that can be analyzed in breeding, genetic, and evolutionary studies of Phaseolus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224994
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  • 8
    Electronic Resource
    Electronic Resource
    Morphogenesis and tissue cultures of three citrus species (1989)
    Springer
    Plant cell, tissue and organ culture 16 (1989), S. 123-133 
    Details
    ISSN: 1573-5044
    Keywords: morphogenesis ; organogenesis ; callus ; root culture ; Citrus sinensis ; Citrus medica ; Citrus aurantifolia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies were performed to define tissue culture techniques and culture conditions for morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis (L.) Osb.), citron (C. medica L.) and lime (C. aurantifolia) (Christm. Swing). The optimal concentrations of NAA to induce root formation on stem segments were 10 mg l-1 for sweet orange and lime, and 3 mg l-1 for citron. The optimal BA concentration for shoot and bud proliferation was 3 mg l-1 for sweet orange and citron, and 1 mg l-1 for lime. Callus initiation was accomplished in a culture medium containing 10 mg l-1 NAA and 0.25 mg l-1 BA. Callus was maintained by periodical subculture into the same medium supplemented with 10% (v:v) organge juice. In vitro plantlets of the three species were obtained by rooting of shoots developed from bud cultures, and of citron and lime by development of shoots from root cultures. The plants were successfully established on soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036520
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  • 9
    Electronic Resource
    Electronic Resource
    Morphogenesis and tissue culture of sweet orange (Citrus sinensis (L.) Osb.): Effect of temperature and photosynthetic radiation (1992)
    Springer
    Plant cell, tissue and organ culture 29 (1992), S. 11-18 
    Details
    ISSN: 1573-5044
    Keywords: callus ; morphogenesis ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Both incubation temperature and photosynthetic radiation affected morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis) cultured in vitro. Bud culture from nodal stem segments, regeneration of shoots and buds from internode stem segments and induction of primary callus were near optimal at incubation temperatures between 21–30°C. The optimal temperature for root formation was 27°C with temperatures above and below being clearly deleterious. Incubation in the dark or under low photosynthetic photon flux density (PPFD) was beneficial for callus induction and growth and also favored the production of rooted plantlets from bud cultures. Incubation in the dark improved considerably the regeneration of shoots and buds from internode segments and the recovery of whole plants. No off-types, as determined by protein and isoenzyme analysis, were observed among plantlets recovered from bud cultures or from regeneration of shoots from internode stem segments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036140
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