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  • 1
    Digitale Medien
    Digitale Medien
    Effect of salinity on growth, ion content and CO2 assimilation rate in lemon varieties on different rootstocks (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Citrus rootstocks as well as lemon scions differ in their ability to restrict sodium and chloride ions and in their sensitivity to saline stress. To determine the behaviour of different rootstock-scion combinations, 3 lemon cultivars on 3 different rootstocks were grown in containers in a greenhouse and irrigated with 5, 25 and 50 mM NaCl. Growth of the plants and foliar contents of sodium and chloride as well as physiological parameters including transpiration rate, gas exchange, stomatal conductance and chlorophyll content were evaluated. Shoot length of the plants on sour orange and on C. volkameriana showed a greater reduction with salinity than those on C. macrophylla. Accumulation of salt in the leaves was also scion dependent, cv. ‘Eureka’ having higher concentrations of sodium and chloride than the others. Assimilation rate of CO2 and stomatal conductance were greatly reduced by salinity in the leaves of Verna and Eureka on sour orange. Gas exchange in the leaves was highly correlated with chloride and sodium contents in all lemon-rootstock combinations. C. macrophylla showed a higher resistance to salinity than C. volkameriana and sour orange. Inferences on the mechanisms of action of salt on lemon trees are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb05194.x
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  • 2
    Digitale Medien
    Digitale Medien
    Growth arrest of Schizosaccharomyces pombe following overexpression of mouse type 1 protein phosphatases (1998)
    Springer
    Molecular genetics and genomics 259 (1998), S. 449-456 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Mouse PP1 ; Schizosaccharomyces pombe ; Overexpression ; Lethal effects ; Deletion mutants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Three mouse genes encoding type 1 protein phosphatase isotypes α, γ and δ were expressed in Schizosaccharomyces pombe cells under the control of the regulatable promoter nmt1. In the repressed state, basal expression of mouse genes was able to rescue the S. pombe mutant dis2-11. An integrated mouse gene could not, however, genetically complement S. pombe double mutants carrying disrupted dis2 and sds21 protein phosphatase genes. Overexpression of any of the three mouse protein phosphatase1 isotypes produced growth arrest and loss of viability in wild-type S. pombe cells. Overexpressing cells showed defects in chromosome distribution and in the formation of septa. These defects are characteristic of the expression of the mammalian protein phosphatases in S. pombe, since they are not observed after overexpression of endogeneous S. pombe type 1 protein phosphatases. Overexpression of a truncated version of isotype δ, which lacked protein phosphatase activity, reproduced most of the characteristics of the lethal phenotype. We conclude that the lethal effect may be due to interactions with essential cell cycle proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004380050835
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  • 3
    Digitale Medien
    Digitale Medien
    Purification of α-hemolysin from an overproducing E. coli strain (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 106-110 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The genetic determinant of the α-hemolysin encoded by plasmid pHly152 has been cloned in both orientations in plasmid pBR322 giving rise to plasmids pSU157 and pSU158. E. coli strains carrying either of these recombinant Hly plasmids produced about 20 times more hemolysin activity than the parental plasmid pHly152, when grown in minimal medium supplemented with hemoglobin. Thus high hemolytic activity is not lethal to the cells, contrary to previous assumptions. α-hemolysin was purified from culture supernatants of strain SU100 (pSU157) by ammonium sulfate precipitation and gel filtration in Sephacryl S-200 in the presence of 6 M urea. When purified α-hemolysin preparations were subjected to electrophoretic analysis in denaturing conditions, a single 107 kdal polypeptide was observed. This probably corresponds to the α-hemolysin protein, since an isogenic E. coli strain carrying plasmid pSU161, an Hly- mutant derivative of pSU157, did not synthesize the 107 kdal polypeptide.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00327518
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  • 4
    Digitale Medien
    Digitale Medien
    Characterization and nucleotide sequence of the orif-traM-finP region of the IncFVII plasmid pSU233 (1992)
    Springer
    Molecular genetics and genomics 234 (1992), S. 442-448 
    Details
    ISSN: 1617-4623
    Schlagwort(e): IncF plasmids ; RepFIIA family ; pSU233 ; Module exchange ; tra alleles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary By hybridizing the IncFVII haemolytic plasmid pSU233 with a probe containing the origin of transfer of the IncFII plasmid R1, we isolated a 1.9 kb BglII fragment containing at least the origin of transfer (oriT), and the genes traM and finP. Functional complementation analysis of deletion derivatives was used to map the origin of transfer. We also determined the nucleotide sequence of traM and finP. Comparison with similar regions of several plasmids, also belonging to the RepFIIA family, revelaed that pSU233 resembles the F plasmid by very close. The homology is not evenly distributed along this region, but clustered into homologous regions (TraZb-oriT, TraMb-oriT and traM separated by non-homologous regions (TraYb-oriT, finP). This organization resembles that reported for the replication region and also suggests evolution by exchange of modules. In addition, the nucleotide sequence of finP is different from those previously described for other IncF plasmids and constitutes a new allele, which we have denominated allele VI.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00538704
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  • 5
    Digitale Medien
    Digitale Medien
    A molecular analysis of transductional marker rescue involving P-group plasmids in Pseudomonas aeruginosa (1976)
    Springer
    Molecular genetics and genomics 143 (1976), S. 333-337 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The molecular properties of the P-group plasmids R26, R527 and R18-18 (a carbenicillin-sensitive derivative of R18) have been compared with those of RP1. R18-18 and RP1 have a MW about 38×106 daltons, and R26 and R527 of 52×106 daltons (determined from contour lengths). All three plasmids have a buoyant density similar to that of RP1 (1.719 g/cm3, 60% G+C). From their molecular and phenotypic similarities, these plasmids probably represent two pairs of identical or closely similar elements. Resistant bacteria are not recovered following F116L-mediated transduction of R26 (or R527), and this correlates with the plasmids' larger size (phage genome=40×106 daltons). Fragments of R26 are, however, transduced and their resistance determinants may be “rescued” by recombination if the recipient harbours R18-18. Such events are accompanied by an increase in the size of the recipient plasmid from 38×106 to 52×106 daltons following inheritance of the resistance determinants Sm Su Gm Hg, but not Cb. Thus, Sm Su Gm Hg are encoded in a DNA segment of MW about 14×106 daltons which apparently has no homologous region on R18-18. Since a piece of DNA of this MW also corresponds to the difference in size between R26 and R18-18, it is possible that the former is derived from an RP1-like element which has acquired these additional resistance determinants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00269412
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