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  • 1
    Electronic Resource
    Electronic Resource
    SV40 Neutralizing Antibodies in Sera of US Residents without History of Polio Immunization (1971)
    [s.l.] : Nature Publishing Group
    Nature 231 (1971), S. 448-449 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We made our observations during a continuing study of cancer of the urinary bladder in a search for possible papova-virus aetiology. Sera from 210 patients with bladder or other types of cancer were screened for antibodies to SV40. The donors comprised 157 white males, thirty-seven white females, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/231448a0
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  • 2
    Electronic Resource
    Electronic Resource
    Enhanced transformation of human fibroblasts by origin-defective simian virus 40 (1982)
    [s.l.] : Nature Publishing Group
    Nature 296 (1982), S. 671-672 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The SV ori mutant is a hybrid DNA consisting of plasmid (pMK16) and the full genome of SV40 DNA less 6 nucleotides at the BgR site8'9. It is able to transform rat cells as efficiently as the ori+ SV40 derivative, but, unlike the latter, is able to transform permissive monkey cells10. The cell line ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/296671a0
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  • 3
    Electronic Resource
    Electronic Resource
    Mechanism of immortalization (1999)
    Springer
    Age 22 (1999), S. 65-69 
    Details
    ISSN: 1574-4647
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Model systems implementing various approaches to immortalize cells have led toward further understanding of replicative senescence and carcinogenesis. Human diploid cells have a limited life span, termed replicative senescence. Because cells are terminally growth arrested during replicative senescence, it has been suggested that it acts as a tumor suppression mechanism as tumor cells exhibit an indefinite life span and are immortal. The generation of immortal cells lines, by the introduction of SV40 and human papillomavirus (HPV) sequences into cells, has provided invaluable tools to dissect the mechanisms of immortalization. We have developed matched sets of nonimmortal and immortal SV40 cell lines which have been useful in the identification of novel growth suppressor genes (SEN6) as well as providing a model system for the study of processes such as cellular aging, apoptosis, and telomere stabilization. Thus, their continued use is anticipated to lead to insights into other processes, which are effected by the altered expression of oncogenes and growth suppressors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s11357-999-0008-1
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  • 4
    Electronic Resource
    Electronic Resource
    A murine cell possessing a dominant mutation affecting the regulation of interferon production: Characterization by intraspecific hybrids (1978)
    Springer
    Somatic cell and molecular genetics 4 (1978), S. 677-697 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using intraspecific hybrids, we have demonstrated the dominant nature of two phenotypic markers present in a mutant mouse 3T6 cell line, designated 3T6-V r B2. These are, resistance to virus infection (V r )and semiconstitutive synthesis of interferon (IF sc ).Hybrids were formed by polyethylene glycol-mediated fusion between 3T6-V r B2, or its parent 3T6, and 2TG0-13, a triply marked derivative of mouse 3T3 cells. When tested for the V r marker, 3T6-V r B2 ×2TG0-13 hybrid clones displayed a level of resistance to virus infection which was equal to or greater than that of 3T6-V r B2. Similarly, when tested for the IF sc marker, these hybrid clones were found to possess the capacity to confer an interferon-induced antiviral state in mouse L 929 cells upon cocultivation. By comparison, clones derived from 3T6 × 2TG0-13 fusions produced high levels of virus and failed to confer an interferon-induced antiviral state in L 929 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01543158
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  • 5
    Electronic Resource
    Electronic Resource
    Temperature-sensitive mutants of Balb/3T3 cells. III. Hybrids betweents2 and other mouse mutant cells affected in DNA synthesis and correction ofts2 defect by human X chromosome (1980)
    Springer
    Somatic cell and molecular genetics 6 (1980), S. 603-614 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Complementation studies were performed withts 2, a mouse 3T3 cell mutant temperature sensitive (ts) for cell and viral DNA synthesis. Thets phenotype is corrected by non -ts mouse or human cells and a non-DNA− ts mutant. This gene had been localized to a region on the human X chromosome near the HPRT locus based on isozyme and karyotype analysis of hybrids. Unusually rapid loss and fragmentation of human chromosomes occurs in hybrids withts 2. Hybrids betweents 2 and other DNA− ts mutants of mouse cells did not show complementation of the growth phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538640
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  • 6
    Electronic Resource
    Electronic Resource
    Defective DNA topoisomerase I activity in a DNAts mutant of Balb/3T3 cells (1985)
    Springer
    Somatic cell and molecular genetics 11 (1985), S. 557-569 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cell and polyomavirus DNA synthesis in ts20, a temperature-sensitive mutant derived from Balb/3T3 cells, is inhibited at an early step in chain elongation in vivo and in vitro. Virus DNA synthesized under restrictive conditions, when analyzed by gel electrophoresis and fluorography, contained a series of equally spaced bands migrating between form I and form II. If restrictive conditions were prolonged, the relative amount of these less-supercoiled topoisomers increased while the overall amount of virus DNA decreased. DNA topoisomerase I activity was lower and more heat-labile when prepared from mutant cells compared to wild-type and revertant cells. An assay in which extracts from wild-type cells corrected defective cell DNA synthesis in lysed mutant cells was applied to purification of the active factor from such extracts. Salt fractionation and three cycles of column chromatography resulted in the isolation of the activity in a fraction containing 10 major polypeptides. The specific activity in the final preparation was increased fivefold and was accompanied by the activity of DNA topoisomerase I. Our results provide evidence that DNA topoisomerase I functions at an early step in chain elongation of cell and polyomavirus DNA synthesis and that the enzyme activity may be decreased as a result of the mutation in ts20.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01534721
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  • 7
    Electronic Resource
    Electronic Resource
    Replication kinetics of three DNA sequence families in synchronized mouse cells (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 90 (1977), S. 337-350 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Balb/C 3T3 cells entered the quiescent G0 state following serum deprivation. On addition of fresh serum, more than 95% of the culture resumed growth, but with asynchronous kinetics. If hydroxyurea were added just before the first cells reached S phase, at least 90% of the cells accumulated at the Gl/S border over the next ten hours. When the block was removed, the culture moved synchronously into S phase. As the cells traversed S, the replication kinetics of three classes of rapidly renaturing DNA were analyzed. Main band highly repeated DNA and foldback DNA replicated continuously. In contrast, satellite DNA replication did not commence until three hours into S, whereupon its rate of synthesis increased very rapidly, reaching a maximum within the next two hours. These results are discussed in the light of earlier work utilizing other methods of cell synchronization.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040900219
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  • 8
    Electronic Resource
    Electronic Resource
    Purine pyrophosphorylase as a selective genetic marker in a mouse lymphoma, P388, in cell culture (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 68 (1966), S. 61-67 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It is possible to use the purine pyrophosphorylase in mammalian cell culture systems as a genetic marker in selecting small numbers of enzyme positive cells from large populations of pyrophosphorylase negative cells of the mouse lymphoma line P388 in medium containing amethopterin, hypoxanthine, glycine and thymidine. Conversely, it is readily possible to obtain pyrophosphorylase-deficient cells by treatment with 8-Azaguanine. We were unsuccessful in demonstrating DNA-mediated transformation using DNA from enzyme positive cells incubated with cells which were enzyme negative.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040680108
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  • 9
    Electronic Resource
    Electronic Resource
    Expression of transformation in cell hybrids. II. Nonsuppression of the transformed phenotype in hybrids between a chemically transformed and nontransformed derivatives of Balb/3T3 (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 97 (1978), S. 147-152 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hybrid clones derived from a nitrosocarbaryl-transformed Balb/3T3 cell line, Clone H, and a nontransformed cell line TH02 resemble the transformed parent in the clone morphology, higher saturation density, colony formation in medium with reduced serum concentration, growth in agarose and ability to form clones on Balb/3T3 monolayer. Results are discussed in the framework of genetic models which permit or require dominant mutations for the expression of transformed phenotype.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040970203
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  • 10
    Electronic Resource
    Electronic Resource
    Ordered replication of DNA sequences: Synthesis of mouse satellite and adjacent main band sequences (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 98 (1979), S. 515-526 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The replication of mouse satellite DNA was delayed when synchronized 3T3 cells were exposed to low concentrations of hydroxyurea during S phase. It appears that the onset of satellite replication is not a time dependent event, but instead requires that a certain amount of main band DNA be synthesized first. Using hydroxyapatite chromatography and S1 nuclease digestion, a procedure was developed to quantitate the synthesis of both satellite and neighboring main band sequences. The replication kinetics of satellite determined by this method agree with previous estimates. Main band sequences adjacent to satellite appear to replicate in concert with satellite DNA. The results are discussed and related to the limitations of the techniques utilized.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040980310
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