ISSN:
0377-0486
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
Resonance Raman spectra have been obtained from dilute solutions of ferri-hemoglobin fluoride, using several Ar+ laser lines, as well as the 6328 Å He — Ne line, for excitation. Several modes (including some with inverse polarization) correspond to vibrations which mix the first two π - π* porphyrin transitions, and their excitation profiles confirm the assignment of the lower of these transitions to a band, at 5280 Å, which is unresolved in the room-temperature absorption spectrum. Resonance enhancement of porphyrin modes with 6328 Å excitation suggests substantial mixing of the lower π - π* transition with porphyrin-metal charge transfer transitions, which presumably account for the low energy visible bands. Totally symmetric modes are observed which come into resonance with the intense near-ultraviolet (Soret) transition. The most intense of these, at 1373 cm-1 accurately obeys the frequency dependence expected for a mode which couples to a single electronic transition.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jrs.1250010207
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