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  • 1
    Electronic Resource
    Electronic Resource
    Nodule-specific glutamine synthetase is expressed before the onset of nitrogen fixation in Phaseolus vulgaris L. (1987)
    Springer
    Plant molecular biology 9 (1987), S. 65-74 
    Details
    ISSN: 1573-5028
    Keywords: glutamine synthetase polypeptides ; nitrogen fixation ; root-nodule development ; nodulins ; Phaseolus vulgaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glutamine synthetase expression was studied in developing root-nodules of common bean with regard to the time-course of specific activity, antigen accumulation, polypeptide composition and in vitro translation products. This analysis shows that the nodule-specific GS polypeptide (GS-gamma) is detected prior to the nitrogenase acetylene-reducing activity, and that its accumulation together with that of the GS-alpha and GS-beta polypeptides vary with nodule age. GS-gamma is present in ineffective nodules, although in a lower ratio to GS-beta than in wild-type nodules. Comparisons of in vitro translated and in vivo synthesized GS polypeptides suggest no post-translational modifications. The possible factors and mechanisms involved in the regulation of expression of GS polypeptides are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017988
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of nodule-specific genes in Phaseolus vulgaris L. (1987)
    Springer
    Plant molecular biology 9 (1987), S. 521-532 
    Details
    ISSN: 1573-5028
    Keywords: gene expression ; nitrogen fixation ; nodulins ; Phaseolus vulgaris ; Rhizobium ; root-nodule development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, nodulespecific uricase and a group that in vitro translates into a cluster of about 30 kDa products. The expression pattern of nodulins in effective (Fix+) nodules compared to ineffective (Fix-) ones is also presented. The modified expression of main nodulins observed between these nodules indicates that different levels and/or factors associated with their regulation are involved. The intracellular infection by Rhizobium as a decisive step in the induction of some P. vulgaris nodulins is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020530
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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