ISSN:
0730-2312
Keywords:
nucclear pore complex
;
nuclear transport
;
Nuclear Proteins
;
Nuclear nvelope
;
facilitated transport
;
nuclear localization signal
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Many proteins - including not only structural proteins, but also enzymes, hormone receptors, and other transcription factors - accumulate to much higher nuclear than cytoplasmic concentrations. Nuclear Localization sequences or signals (NLSs) within their primary structures entrain specific transport of these proteins through the nuclear pore complexes. Thios transport process is energy-dependent, but evidence for a true active transport mechanism is not conclusive. An alternative mechanism - facilitated transport of NLS proteins followed by their intranuclear binding - has been implicated by experiments with oil-isolated nuclei. However, there has been no agreement as to a role for binding in the in vivo nuclear accumulation of NLS-containing proteins. We Demonstrate herein that a prototypical NLs protein, Nucleoplasmin (NP), binds within the nucleus of the living Xenopus oocyte and that this binding accounts for its nuclear accumulation. © Wiley-Liss, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240580113
Permalink
