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  • 1
    Electronic Resource
    Electronic Resource
    Topography of growth hormone receptor expression in the bovine embryo (1998)
    Springer
    Histochemistry and cell biology 109 (1998), S. 417-419 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Using in situ hybridization, mRNA encoding the growth hormone receptor (GHR) was localized in preimplantation embryos produced by in vitro fertilization (IVF) as well as in 30- to 70-day-old bovine embryos. In IVF embryos the transcript of GHR was demonstrated in the inner cell mass of 8-day-old blastocysts. In 30-day-old embryos, the mesonephros was the first organ to express the mRNA of GHR. In 40-day-old embryos, the transcript was found in the neurones of the spinal ganglions, the splanchnic nerves and the motoneurones of the spinal cord, in the vascular endothelium, and in the developing striated muscle tissue. Colocalization of the protein by immunohistochemistry showed an identical distribution pattern of GHR in 30- to 70-day-old embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050243
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers (1996)
    Springer
    Journal of assisted reproduction and genetics 13 (1996), S. 259-265 
    Details
    ISSN: 1573-7330
    Keywords: laser micromanipulation ; laser-assisted fertilization ; laser zona drilling ; laser-mediated subzonal sperm insertion ; microinsemination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes. Method: A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser drilled oocytes. Results: Fertilization was achieved only when three to five cattle sperm were trapped with optical tweezers and inserted directly through the laser drilled hole into the perivitelline space. After 20 hr, 3 of 79 (3.8%) oocytes revealed two pronuclei and a sperm tail within their cytoplasm. Cattle sperm are difficult to catch. Therefore, the gametes had to remain for about 20 min in room atmosphere, which might be the reason for the low fertilization results. Conclusions: The results indicate that a combined UV-laser microbeam and optical tweezers trap can be used successfully for “noncontact” microinsemination procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02065947
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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