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  • 1
    Electronic Resource
    Electronic Resource
    Large-scale plant cell culture: methods, applications and products
    Amsterdam : Elsevier
    Current Opinion in Biotechnology 2 (1991), S. 370-374 
    Details
    ISSN: 0958-1669
    Keywords: [abr] SIPC; surface-immobilized plant cell
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0958-1669(05)80140-5
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  • 2
    Electronic Resource
    Electronic Resource
    Progress of Polymerization of D,L-Lactide Through Differential Scanning Calorimetry and Gel Permeation Chromatography (1998)
    Springer
    Journal of thermal analysis and calorimetry 52 (1998), S. 559-564 
    Details
    ISSN: 1572-8943
    Keywords: D,L-lactide ; tetraphenyltin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Melt polymerization conditions for D,L-lactide initiated with tetraphenyltin were studied with regard to polymer molecular weight. The present study was undertaken to investigate the progress of polymerization of D,L-lactide through differential scanning calorimetry (DSC), and also to explore the correlation between melt polymerization conditions and molecular weight. The physical characteristics, such as glass transition temperature (Tg) of the polymer and melting transition (Tm) of D,L-lactide are correlated with GPC data. DSC data shows that the Tm of D,L-lactide is 122.8 at 150°C polymerization time. ΔHf is 83.2 J g-1, and Tg of polymer is untraceable. At 180°C the Tm is 101.4°C, ΔHf is 34 J g-1, and Tg is around 29.5°C. The drop in Tm and ΔHf clearly shows the conversion of D,L-lactide to polymer. The maximum increment to molecular weight of polymer is achieved at 160°C and 8 h. After a short induction period, the slow polymerization of D,L-lactide resulted in maximal molecular weight followed by an almost constant value of molecular weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010171606881
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  • 3
    Electronic Resource
    Electronic Resource
    Influences of yeast extract on specific cellular yield of Ovine growth hormone during fed-batch fermentation of E. coli (2000)
    Springer
    Bioprocess engineering 22 (2000), S. 379-383 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  In most cases of E. coli high cell density fermentation process, maximizing cell concentration helps in increasing the volumetric productivity of recombinant proteins usually at the cost of lower specific cellular protein yield. In this report, we describe a process for maintaining the specific cellular yield of Ovine growth hormone (oGH) from E. coli by optimal feeding of yeast extract during high cell density fermentation process. Recombinant oGH was produced as inclusion bodies in Escherichia coli. Specific cellular yield of recombinant oGH was maintained by feeding yeast extract along with glucose during fed-batch fermentation. Glucose to yeast extract ratio of 0.75 was found to be optimum for maintaining the specific cellular oGH yield of 66 mg/g of E. coli cells. Continuous feeding of yeast extract along with glucose helped in reducing acetic acid secretion and promoted higher cell growth during fed-batch fermentation. High cell growth of E. coli and high specific yield of recombinant oGH thus helped in achieving high volumetric productivity of the expressed protein. A maximum of 2 g/l of ovine growth hormone was expressed as inclusion bodies in 12 h of fed-batch fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004490050747
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation and lattice constant measurement of (Ca + Cd + Pb) hydroxylapatites (1991)
    Springer
    Journal of materials science 10 (1991), S. 638-639 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00723363
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  • 5
    Electronic Resource
    Electronic Resource
    Alkaloid production by plant cell cultures of Holarrhena antidysenterica: II. Effect of precursor feeding and cultivation in stirred tank bioreactor (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 39 (1992), S. 1052-1057 
    Details
    ISSN: 0006-3592
    Keywords: Holarrhena antidysenterica ; suspension culture ; conessine ; precursor feeding ; stirred tank reactor ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Precursor feeding strategy for increasing the yield of conessine, a steroidal alkaloid of Holarrhena antidysenterica, was established in cell suspension culture. A total of 50 mg/L added cholesterol was converted into 43 mg/L of alkaloid, 90% of which constituted the conessine. By applying the precursor feeding policy to the cell suspension culture in modified Murashige and Skoog (MS) medium, a total of 143 mg/L of alkaloid was produced in 8 days. In this way the alkaloid content of the cells was increased more than six times compared to that obtained in the standard MS medium. The steps leading to biotransformation of cholesterol into alkaloids were unaffected by phosphate. The shake flask data were successfully transferred to a bench scale 6-L stirred tank bioreactor in which the specific biosynthetic rate of alkaloid production was 110 mg/100 g dry cell weight per day, about 160 times higher than that of whole plant.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260391009
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  • 6
    Electronic Resource
    Electronic Resource
    Alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica: I. Effect of major nutrients (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 39 (1992), S. 1043-1051 
    Details
    ISSN: 0006-3592
    Keywords: Holarrhena antidysenterica ; plant cell suspension culture ; alkaloid ; conessine ; macro nutrients ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of major nutrients on growth and alkaloid production by plant cell culture of Holarrhena antidysenterica was studied with a view to increasing the yield of the alkaloid conessine, a therapeutic drug used for treatment of dysentery and helminthic disorders. The studies resulted in development of a modified Murashige and Skoog (MS) medium that contained 60 mM total nitrogen with a NH4+-to-NO3- ratio of 5:1, 0.25 mM phosphate, and 40 g/L sucrose. The growth regulators 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (Kn) were also found to affect the synthesis of alkaloid. Using an optimal level of inoculum (3 g/L), the modified medium resulted in alkaloid synthesis of 0.66 g/100 g dry cell weight, which represented a 4.25-fold increase over that obtained in standard MS medium.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260391008
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  • 7
    Electronic Resource
    Electronic Resource
    Micellar effect on the polymerization. III.Part 1: H. K. Das and B. C. Singh, J. Appl. Polym. Sci., 49, 1107-1113 (1993). Part 2: C. M. Patra and B. C. Singh, J. Appl. Polym. Sci., 52, 1549-1556 (1994). kinetics of polymerization of acrylonitrile initiated by Ce(IV)-glucose system in presence of cationic micelles (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 58 (1995), S. 1999-2003 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The micellar effect on the kinetics of ceric ion-initiated polymerization of acrylonitrile (AN) in the presence of glucose was studied in the temperature range 40-50°C. The effects of concentration of the metal ion, monomer, sulfuric acid, substrate (glucose), emulsifier, some inorganic salt, and organic solvent on the rate of polymerization (Rp) were also investigated. The remarkable features of the investigation involve the enhancement of Rp in the presence of a micelle, the derivation of the rate expression for the above system along with the calculation of activation energy, and prediction of optimum conditions. Comparison of the rates for a micellar medium with those for the aqueous medium indicates that cationic micelles exert an accelerating influence on the rates of polymerization. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070581110
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