ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Background: Both passive and active dressings and technologies are proposed for treatment of chronic diabetic wounds. A quantitative assessment of healing is needed. Measurement of scattering and absorption characteristics of healing tissue, by Diffuse Near Infrared Spectroscopy (NIR) may provide such a method.Methods: 20 rats were divided into an induced diabetic and a control group (N = 10 each). Full thickness wounds were made on the dorsal surface and biopsies sent for histologic analysis.Wounds were interrogated at twice per week intervals using a frequency domain near infrared device with three wavelengths of incident light. Amplitude and phase of scattered light were obtained at four different source-detector distances at each wavelength. The probe was positioned at six different locations. Tissue absorption and reduced scattering coefficients were calculated from amplitude and phase data. Wound dimensions were calculated by cross and parallel polarization.Results: Thirty-eight wounds were evaluated during the experiment. Wound size decreased at twice the rate in control. Average absorption coefficient was higher, by a factor of 2, in diabetics. The average value of reduced scattering coefficient was 30% higher in diabetics as well. During healing, both scattering and absorption coefficient increased faster in diabetics.Discussion: Higher absorption in diabetic rats suggest impaired blood flow. Scattering reflects tissue disorganization observed in delayed wound healing.Conclusion: These data suggest that near-infrared spectroscopy can detect the difference in oxygen delivery to the wound and assess wound healing progression. Further analysis is planned to investigate pathologic correlates with spectroscopic data.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130215j.x
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