ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Tryptophan
;
Chiral separations
;
Diode-laser polarimetric detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The combined utilization of photometric, fluorimetric and polarimetric detection liquid chromatography detectors in series for the identification and quantification of D-tryptophan and L-tryptophan was evaluated. Detection limits of about 1 μg were established with the range of linearity extending to about 100 μg. The relative standard deviation of the D-form and L-form tryptophan were 7.32 and 4.22%, respectively. The amount unknown of tryptophan enantiomers in the different mixtures was determined with an accuracy of 1.58% at the 40 μg injection level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290282
Permalink