ISSN:
1432-072X
Keywords:
Pyrococcus furiosus
;
Hyperthermophilic archaea
;
Maltose fermentation
;
Peptide fermentation
;
Tungsten
;
α-Glucosidase
;
Aldehyde ferredoxin oxidoreductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pyrococcus furiosus is a strictly anaerobic heterotroph that grows optimally around 100 °C. It can be cultured in an artificial seawater-based medium with either peptides or maltose as the carbon source. Significant stimulation of cell yields were observed when trace levels of tungsten (as tungstate) were added to an energy-limited chemostat culture of P. furiosus when maltose is present, but not when peptides were the sole carbon source. The addition of tungsten also led to dramatic increases in the specific activities within cell-free extracts of aldehyde ferredoxin oxidoreductase (AOR), which is a tungsten-iron-sulfur protein. Moreover, the addition of tungsten to cells growing in maltose/peptide medium dramatically reduced the specific activity of intracellular proteases, suggesting a preference for the utilization of maltose over peptides as the carbon and energy source in the presence of tungsten.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290921
Permalink