ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: Cells dissociated from the forebrains of 21-day-old rats were enriched in oligodendroglia by Percoll gradient centrifugation, seeded on polylysine-coated surfaces, and cultured in a serum-containing medium. Incorporation by the cultures of tritium from d-[3H]galactose into the galactosyl residue of galactocerebroside (galC) increased in an almost linear fashion for 48 h with 1–8 μCi of d-[3H]galactose (30 mCi/μmol) per milliliter medium. Treatment for 2 days (day 1–3 after seeding) with 10−4M or 10−3M dibutyryl cyclic adenosine 3′,5′-monophosphate (db cyclic AMP) or 10−4M 8-bromo cyclic AMP stimulated galC radiolabelling. Incorporation of d-[3H]galactose into galC during a terminal 48-h radiolabelling period was not stimulated when the cells were continuously treated with these cyclic AMP analogues for 8 rather than 2 days.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1986.tb12963.x
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