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  • 1
    Electronic Resource
    Electronic Resource
    Proliferation kinetics of mussel (Mytilus galloprovincialis) gill cells (1994)
    Springer
    Marine biology 120 (1994), S. 41-45 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique of sister chromatid differentiation (SCD) using bromodeoxyuridine (BrdU) incorporation and a modification of the fluorescence plus Giemsa (FPG) method was employed to determine cell-proliferation kinetics in gill tissue of the mussel Mytilus galloprovincialis. Dose-dependent proliferation inhibition was examined. In vivo administration of BrdU for 12, 24, 36, 48, 60, 72, 84, and 96 h was studied. Our data show that the highest yield of second-generation metaphase plates is obtained after 60 h BrdU treatment; the duration of a cell cycle is 24 to 30 h. On the basis of these data, a BrdU incorporation period of 48 to 60 h would seem to be most appropriate for the sister chromatid exchange (SCE) tests carried out in gill cells of M. galloprovincialis, whereas the 12 to 24 h exposure would give the best results for replication band analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381940
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  • 2
    Electronic Resource
    Electronic Resource
    Banding pattern of mussel (Mytilus galloprovincialis) chromosomes induced by 2 × SSC/Giemsa-stain treatment (1990)
    Springer
    Marine biology 106 (1990), S. 375-377 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mytilus galloprovincialis were collected from an intertidal population in NW Spain in 1988, and chromosomes from the gill tissue of 37 individuals were studied. The present paper describes the banding pattern of aM. galloprovincialis population which enabled us to identify all pairs of chromosomes. Banding was induced by means of a 2 × SSC (0.3M sodium chloride:0.03M sodium citrate)/Giemsa-staining technique, and a diagrammatic representation was constructed based on mean number of bands. The application of this banding technique may prove useful in future research related to the cytogenetics and cytotaxonomy of mussels and other molluscs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01344315
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The R-banding pattern of the chinese hamster Don cell line (1986)
    Springer
    Genetica 78 (1986), S. 51-55 
    Details
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chinese hamster cells (Don line) were treated in vivo with 5-BrdU and 332580Hoechst fluorochrome for obtaining the partial inhibition of condensation that causes the R-banding pattern. Untreated chromosomes were stained by a standard G-banding method. Statistical measurements show significant differences in the band numbers between the two treatments. The Don cell line in the authors' laboratory presents some karyotypical differences from Don cell lines studied by other authors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00058674
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of NORs and NOR-associated heterochromatin in the mussel Mytilus galloprovincialis Lmk (1997)
    Springer
    Chromosome research 5 (1997), S. 268-273 
    Details
    ISSN: 1573-6849
    Keywords: fluorochrome staining ; heterochromatin ; in situ hybridization ; Mytilus galloprovincialis ; nucleolar organizing regions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosomes of the mussel Mytilus galloprovincialis were analysed by means of chromomycin A3 (CMA), distamycin A/DAPI (DA/DAPI), DAPI/actinomycin D (DAPI/AMD) and chromomycin A3/distamycin A/DAPI(CDD) fluorescence banding techniques, C-banding, silver staining, N-banding and in situ hybridization with 18S+28S rDNA and telomere probes. 18S+28S rDNA clusters were located on the telomeres of two pairs of submeta/subtelocentric chromosomes. The nucleolar organizing regions (NORs) were associated with bright CMA fluorescence, dull DAPI fluorescence and C- and N-positive bands, but not all four NOR-associated heterochromatin bands showed bright CMA fluorescence in a given cell; intra- and interindividual variability was found in this character. Additional non-ribosomal C-bands did not show any differential fluorescent behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018475804613
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Cytogenetics in Brachidontes rodriguezi d'Orb (Bivalvia, Mytilidae) (1999)
    Springer
    Chromosome research 7 (1999), S. 49-55 
    Details
    ISSN: 1573-6849
    Keywords: Bivalvia ; Brachidontes rodriguezi ; fluorescent in-situ hybridization (FISH) ; fluorochrome staining ; nucleolus organizing regions (NORs) ; replication banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosomes of Brachidontes rodriguezi were analysed by means of direct Giemsa staining, silver staining, fluorescent in-situ hybridization (FISH) with 18S + 28S rDNA probes, replication banding and chromomycin A3 (CMA) and DAPI fluorescence banding techniques. The diploid chromosome number in this species is 32 and the karyotype is composed of two pairs of metacentric chromosomes, 2 pairs of telo/subtelocentric chromosomes and 12 pairs of subtelocentric chromosomes. 18S + 28S rDNA clusters were located on the short arms of the two pairs of telo/subtelocentric chromosomes. The replication band pattern induced in this species facilitates chromosome pairing and differentiation. The nucleolar organizing regions (NORs) replicate late in the S phase and were associated with bright CMA fluorescence and dull DAPI fluorescence, but not all the four NORs showed bright CMA fluorescence in a given cell; intra- and interindividual variability was found for this character.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009275311888
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  • 6
    Electronic Resource
    Electronic Resource
    Stable methylation patterns in interspecific antelope hybrids and the characterization and localization of a satellite fraction in the Alcelaphini and Hippotragini (2000)
    Springer
    Chromosome research 8 (2000), S. 635-643 
    Details
    ISSN: 1573-6849
    Keywords: antelope hybrids ; FISH ; methylation ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conflicting data has recently appeared concerning altered methylation patterns in interspecific mammalian hybrids and the potential this may hold for driving karyotypic evolution. We report no detectable methylation difference in the genomic DNA of different interspecific F1 antelope hybrids (family Bovidae) and their parent species using the methylation-sensitive enzyme HpaII and its methylation insensitive isoschizomer MspI. However, both enzymes released a tandemly repeated satellite array. Characterization of the repeat using Southern blotting and a combination of sequencing, fluorescence in-situ hybridization (FISH) and C-banding, shows some similarity in the family of repeats between the hybridizing antelope species groups, and that the satellite is localized in the centromeric C-band positive regions of the chromosomes. Moreover, although there is little meaningful sequence homology with the well characterized bovine 1.715 satellite DNA, there is 86% sequence similarity with the sheep/goat satellite I, suggesting that they are related and are likely to have originated and evolved separately from the bovine unit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009294226213
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    Paper (German National Licenses)
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