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  • 1
    Electronic Resource
    Electronic Resource
    The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene (1992)
    Springer
    Plant molecular biology 19 (1992), S. 611-622 
    Details
    ISSN: 1573-5028
    Keywords: anther ; Arabidopsis thaliana ; Brassica napus ; tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Brassica napus cDNA clone A9 and the corresponding Arabidopsis thaliana gene have been sequenced. The B. napus cDNA and the A. thaliana gene encode proteins that are 73% identical and are predicted to be 10.3 kDa and 11.6 kDa in size respectively. Fusions of an RNase gene and the reporter gene β-glucuronidase to the A. thaliana A9 promoter demonstrated that in tobacco the A9 promoter is active solely in tapetal cells. Promoter activity is first detectable in anthers prior to sporogenous cell meiosis and ceases during microspore premitotic interphase. The deduced A9 protein sequence has a pattern of cysteine residues that is present in a superfamily of seed plant proteins which contains seed storage proteins and several protease and α-amylase inhibitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026787
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Patterns of gene expression in developing anthers of Brassica napus (1991)
    Springer
    Plant molecular biology 17 (1991), S. 195-207 
    Details
    ISSN: 1573-5028
    Keywords: anther development ; microspore ; tapetum ; cDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between bud length, anther length and stage of anther development has been investigated in Brassica napus using a series of cytological markers that define steps in the process of male gametogenesis. It was determined that bud length is directly related to anther length and that anther or bud length is tightly linked to the stage of male gametogenesis within the anther. This simple correlation has enabled the construction of cDNA libraries representing transcripts expressed in defined stages of anther development, and the detailed examination of the developmental pattern of expression of anther RNAs. Two anther cDNA libraries were constructed, one from anthers of 1.2–1.8 mm long buds (sporogenesis library) and one from anthers of 1.8–4.0 mm long buds (microspore development library). A total of 19 independent cDNAs have been isolated by differential screening whose temporal expression patterns overlap and which together cover the stages of anther development from pre-meiotic microsporocytes to tri-nucleate pollen grains. The pattern of expression of each of these clones is unique and indicates that stages of anther development which cannot be easily distinguished by light microscopy can be recognised by virtue of the absence or presence of certain RNAs. Three cDNAs isolated from the sporogenesis library have been shown by in situ hybridisation to be tapetum-specific. In contrast, five clones isolated from the microspore development library are microspore-specific. These clones exhibit a pattern of expression different to those previously described in that their transcripts are absent in mature pollen grains. Thus these RNAs are probably required in microspore development rather than for the growth of the germinating pollen grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039494
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The highly expressed tapetum-specific A9 gene is not required for male fertility in Brassica napus (1994)
    Springer
    Plant molecular biology 24 (1994), S. 97-104 
    Details
    ISSN: 1573-5028
    Keywords: anther ; antisense RNA ; Brassica napus ; male fertility ; tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. napus. A high proportion of the offspring of B. napus antisense A9 plants had very low or undetectable levels of A9 mRNA. However, these plants set seed and had pollen of normal or near normal viability. Therefore, under the conditions studied, the A9 protein appears not to be essential for male fertility in B. napus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040577
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The restoration of fertility in male sterile tobacco demonstrates that transgene silencing can be mediated by T-DNA that has no DNA homology to the silenced transgene (2000)
    Springer
    Transgenic research 9 (2000), S. 91-102 
    Details
    ISSN: 1573-9368
    Keywords: anther ; promoter ; restoration of fertility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male sterile tobacco plants expressing a pathogenesis-related (PR) β-1,3-glucanase gene driven by the Arabidopsis thaliana A3 or A9 tapetum-specific promoter, were partially restored to fertility by retransformation with a range of pA9-driven sense and antisense PR glucanase fragments. The restored plants exhibited improved seed set. PR glucanase protein was undetectable in the anthers of these plants and there was an associated increase in microsporocyte callose, the structural target of the A3 and A9-driven PR glucanase. This phenotype was not solely dependent on interactions between sense and antisense PR glucanase transcripts since a pA9-driven restorer was also capable of down regulating a pA3-GUS construct in the absence of extensive promoter, coding region, or terminator sequence homology. Since the A3 and A9 promoters have similar temporal and spatial expression patterns, it is possible that trans-acting factors common to both promoters become limiting in the PR glucanase double transformants resulting in improved levels of fertility. An alternative hypothesis is that additional sequences present in both the silencing and target T-DNAs can mediate the silencing of adjacent non-homologous transgenes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008992619413
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    Paper (German National Licenses)
    Fulltext
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