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  • 1
    Electronic Resource
    Electronic Resource
    Porcine tumor necrosis factor alpha: cloning with the polymerase chain reaction and determination of the nucleotide sequence
    Amsterdam : Elsevier
    Gene 81 (1989), S. 185-191 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; amino acid sequence homology ; amplification ; cachectin ; complementary DNA ; evolution
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(89)90350-8
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis
    Amsterdam : Elsevier
    Gene 102 (1991), S. 171-178 
    Details
    ISSN: 0378-1119
    Keywords: Genomic library ; TNF-α, TNF-β ; hemorrhagic fever ; recombinant DNA ; repetitive sequences ; sequence comparison
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90075-M
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and expression in mammalian cells of porcine tumor necrosis factor alpha: examination of biological properties
    Amsterdam : Elsevier
    Veterinary Immunology and Immunopathology 38 (1993), S. 57-74 
    Details
    ISSN: 0165-2427
    Keywords: [abr] ASF; African swine fever ; [abr] DEP; diethylpyrocarbonate ; [abr] EMEM; Eagle's minimal essential medium ; [abr] HIV; human immunodeficiency virus ; [abr] IU; International Units ; [abr] LPS; lipopolysaccharide ; [abr] MTT; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ; [abr] PBMC; peripheral blood mononuclear cells ; [abr] PCR; polymerase chain reaction ; [abr] TNF-α; Tumor necrosis factor alpha ; [abr] cDNA; complementary DNA ; [abr] ddH"2O; double distilled water ; [abr] pTNF-α; porcine tumor necrosis factor α
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0165-2427(93)90113-I
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 316-319 
    Details
    ISSN: 1420-9071
    Keywords: Lentivirus ; caprine arthritis-encephalitis virus ; maedi-visna virus ; polymerase chain reaction ; DNA ; protein ; virus evolution ; immunoblotting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The polymerase chain reaction (PCR) was used to demonstrate proviral DNA of lentiviruses of small ruminants in cultured cells. Primers for the Taq polymerase were selected in the GAG gene of Icelandic maedi-visna virus and POL gene of caprine arthritis-encephalitis (CAE) virus. Using PCR, proviral DNA of CAE virus was detected at 1 day post infection, 4 days beforeviral protein could be demonstrated using a sensitive immunoblotting protocol and 6 days before the appearance of syncytia. Primers derived from the published sequence of CAE virus successfully primed for the synthesis of homologous virus and Icelandic maedi-visna viruss but not for maedi-visna virus isolated in The Netherlands. In contrast, primers derived from the GAG region of Icelandic maedi-visna virus allowed the amplification of DNA of homologous virus, maedi-visna virus isolated in The Netherlands as well as CAE virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951776
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitative and qualitative evaluation of nine different extraction methods for nucleic acids on soya bean food samples (1998)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 207 (1998), S. 81-90 
    Details
    ISSN: 1431-4630
    Keywords: Key words Genetically modified organisms ; Food ; Polymerase chain reaction ; Tofu ; Lecithin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Deoxyribonucleic acids (DNA) and ribonucleic acids (RNA) as normal compounds of many foodstuffs have so far been of minor interest with respect to human and animal nutrition. Through the approval of various genetically modified crops in the United States and Europe in recent years, these nucleic acids (NA) have become an important tool in food analysis. The reason for this is that in most cases the discrimination between a genetically modified foodstuff and an unmodified one can best be achieved directly at the DNA level by means of proving the presence or absence of the introduced gene(s). So far, the various methods that can be used to purify DNA for such types of analysis have rarely been compared in a comprehensive manner. In this work the effects of nine different extraction methods on yield and quality of previously purified high-molecular-weight DNA from soya beans and of total NA from tofu, soya flour and lecithin have been tested. Quantification was accomplished using UV absorption at 260 nm and quality assessment of the DNA was done by polymerase chain reaction (PCR) with the soya bean-specific lectin 1 system. Using this approach we assessed the limits of DNA amplification for each food sample extracted using different extraction protocols. It was demonstrated that extraction methods using NA-binding resins like Wizard or DNeasy resulted in comparatively low yields. However, the DNA extracted with these methods is of high quality and suitable for downstream processing like PCR. Simpler and faster methods resulted in relatively high yields of NA but of relatively poor quality. Inhibition of PCR was observed due to one of the buffers used during NA extraction. Finally, the different extraction methods used in this study were compared with respect to the costs and the time needed to perform the extractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050299
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Detection of DNA in soybean oil (1998)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 207 (1998), S. 264-267 
    Details
    ISSN: 1431-4630
    Keywords: Key words Soybean oil ; Labelling of GMO ; Removal of DNA ; Oil processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The presence of DNA in foodstuffs which are, or contain, genetically modified organisms (GMO) is the basic requirement for labelling of GMO food in Switzerland and is also being discussed as a requirement for labelling in the European Union. The present work presents data indicating that no genetic material can be recovered after the first processing steps of soybean oil, i.e. when crude soybean oil is simply centrifuged. This fact is of some relevance because centrifugation is one of the first steps in industrial oil processing. A nested PCR system utilising a single copy gene (lectin 1) showed that centrifugation purified the oil from the genetic material by at least a factor of 10 000. The same results were observed when industrially processed oil fractions were analysed. Thus, with respect to the presence of DNA, soybean oil from GMO soybeans is identical to traditional oil and does not need to be labelled as a GMO product in Switzerland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050330
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for false-positive 35S-promoter screening results (2000)
    Springer
    European food research and technology 210 (2000), S. 367-372 
    Details
    ISSN: 1438-2385
    Keywords: Key words Cauliflower mosaic virus ; 35S promoter ; Genetically modified organisms ; Food analysis ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Today DNA-based techniques are very common for the detection of genetically modified organisms (GMOs) in food products. For fast and easy detection of GMOs, polymerase chain reaction (PCR) screening methods, which amplify common transgenic elements, are applied in routine analysis. These techniques do not allow differentiation between GMOs and the natural occurrence of transgenic elements, such as the 35S-promoter of cauliflower mosaic virus (CaMV) or the NOS-terminator of Agrobacterium tumefaciens, and thus may result in false-positive detection of GMOs. In this study we evaluated three different existing 35S screening systems and report the development of two new CaMV-specific PCR systems. These PCR systems based on CaMV-specific genes allow the identification of positively screened 35S food samples as naturally virus-infected products or plants. Seven food samples tested positive in routine 35S screening analysis and negative in GMO specific systems were investigated using the new virus-specific PCR systems. In all seven samples CaMV was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050565
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Specific interactions of histone H1 and a 45 kilodalton nuclear protein with a putative matrix attachment site in the distal promoter region of a cell cycle-regulated human histone gene (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 139 (1989), S. 320-328 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Protein-DNA interactions within the promoter of a cell cycle-regulated human H4 histone gene were examined by binding of 5′-end-labeled DNA segments to Western blots of nuclear protein fractions. Specific protein interactions were observed with DNA segments located between -500 bp and -1,070 bp upstream of the ATG initiation codon and included a histone H1 binding segment flanked on both sides by binding sites for a 45 kD nuclear protein. This region of the gene contains a DNase I-sensitive site in the center (-720 to -820 bp), and sequence analysis revealed the presence of scaffold attachment sequences in the two flanking segments. Topoisomerase II consensus sequences and in vitro topoisomerase II cleavage sites were also detected in the two flanking segments. Our results suggest that the 45 kd nuclear protein may preferentially interact with these two segments of the H4 histone gene to mediate association with the nuclear matrix. The presence of negative regulatory elements in this putative matrix attachment region provides a basis for the speculation that such nuclear proteins are associated with alterations in gene-matrix interaction that are functionally related to gene expression.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041390214
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