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  • 1
    Electronic Resource
    Electronic Resource
    An Immunocytochemical Study of the Luteinizing Hormone-Releasing Hormone (LHRH) System in the White-Footed Mouse: Effect of Blinding and Melatonin (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of pineal research 1 (1984), S. 0 
    Details
    ISSN: 1600-079X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Previous studies have provided evidence that pineal-mediated gonadal atrophy involves the luteinizing hormone-releasing hormone (LHRH) system in some rodents. The purpose of the present study was to visualize the LHRH system in the brain of the white-footed mouse by immunocytochemical methods and to assess the influence of blinding or melatonin on the LHRH immunostaining characteristics of the median eminence. Six intact adult female mice and six adult males (two intact, two blinded, and two with subcutaneous melatonin-beeswax implants) treated for 6 wk were sacrificed by perfusion of fixative, and their brains were removed, cut into 60-μm sections and stained for LHRH by the peroxidase-antiperoxidase (PAP) method. Since the tissues were batch-processed in separate wells in a common chamber, semiquantitive comparisons may be made between similar sections from animals subjected to different treatments. The testes were also collected and prepared for microscopic examination. The distribution of LHRH-stained cell bodies and fibers was found to be essentially the same as has been described in other rodents, i.e., predominantly confined to the preoptic area and hypothalamus. The perikarya appear as unipolar and bipolar fusiform cells with “beaded fiber” type processes. In sections that included the median eminence of the blinded and melatonin-treated males, the degree of immunopositive staining appears to be greater than in the median eminence of untreated mice. The histological appearance of the testes from the treated animals revealed a profound inhibition of gametogenesis. These observations support the suggestion that pineal-mediated gonadal atrophy is the result of an inhibition of LHRH release from the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-079X.1984.tb00227.x
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  • 2
    Electronic Resource
    Electronic Resource
    The primate median eminence II (1976)
    Springer
    Cell & tissue research 175 (1976), S. 265-277 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Macaca mulatto ; High-voltage electron microscopy ; Neurosecretion ; Ependymal cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the normal median eminence of the male rhesus monkey (Macaca mulatta) is described using high-voltage electron microscopy. Surface specializations of ependymal cells lining the infundibular recess included cilia, apical extrusions, and microvilli. Supraependymal cells were predominantly macrophage-like, but examples of lymphocytic types were also seen. Tanycytes had long, branching, basal processes filled with numerous microtubules, some lipid droplets, and granules. The zona interna was composed of large unmyelinated neurosecretory fibers. A few myelinated fibers were also seen, but their character as neurosecretory fibers could not be established. The zona externa was composed of densely-packed profiles of neurosecretory fibers of small diameter, was well-vascularized and contained the terminations of tanycytes. Perivascular glial cells, vesiculated elements, pituicytes, and cellular elements common to connective tissue were observed. The intricate relationships between both the cellular and fibrous elements of the median eminence can be appreciated with the capability of high-voltage electron microscopy to discern ultrastructure in sections 10 times thicker than those used for low-voltage electron microscopy. The median eminence of this primate species has an ultrastructural organization similar to that described for most other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232082
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  • 3
    Electronic Resource
    Electronic Resource
    The tanycyte of the rat median eminence (1979)
    Springer
    Cell & tissue research 200 (1979), S. 329-334 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Axon terminals ; Tanycytes ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present ultrastructural study proves the existence of nerve terminals closely apposed to the plasmalemmata of tanycytes in the rat median eminence. Several of these “axo-tanycytic” endings display remarkable accumulations of agranular endoplasmic reticulum in the form of pleomorphic vesicles which are closely apposed on either side of the plasma membrane of each cell compartment. Some of these vesicular profiles give the impression of structural continuity across both membrane systems. This phenomenon is discussed in the context of being a potential substratum for communication between both cell compartments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236425
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  • 4
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of the human cerebral ventricular system (1973)
    Springer
    Cell & tissue research 139 (1973), S. 61-68 
    Details
    ISSN: 1432-0878
    Keywords: Fourth ventricle ; Human ; Ependyma ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy was used to assess the ultrastructural differences exhibited by the varigated ependymal lining of the near-term human fetal 4th ventricle. The central portion of the fourth ventricular floor, including the median sulcus is punctuated by numerous clumps of cilia. The density of cilia here is not as great as that described for other regions of the human cerebral ventricular system; accordingly, underlying substructure can be noted. There are distinct differences between ependymas that line the floor of the fourth ventricle with those of the adjacent area postrema. The latter region possesses not cilia, but instead exhibits a dense knap of microvilli. The ultra-architecture of the choroid plexus is relatively similar to that of other circumventricular organs with the exception that it possesses small isolated groups of cilia as well as microvilli. These findings are discussed with respect to the dynamics of local CSF movement and flow, ependymoabsorption and ependymosecretion
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307461
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  • 5
    Electronic Resource
    Electronic Resource
    A comparative scanning electron microscopic analysis of the human cerebral ventricular system (1972)
    Springer
    Cell & tissue research 132 (1972), S. 203-215 
    Details
    ISSN: 1432-0878
    Keywords: Third ventricle ; Human ; Ependymal surfaces ; Scanning microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A scanning electron microscopic analysis of the adult human third ventricular wall revealed ultra-architectural differences between dorsal and ventral portions. In the brains of thirteen and sixteen week old human fetuses regional differences in the surface organization of lining ependyma were more sharply defined than those of the adult. Alterations in the luminal surfaces of ependyma may reflect differences in the functional capacity of various ventricular areas. The potential role of certain ependyma (tanycytes) and their putative participation in neuroendocrine events is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307011
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural analysis of the human cerebral ventricular system (1974)
    Springer
    Cell & tissue research 150 (1974), S. 389-397 
    Details
    ISSN: 1432-0878
    Keywords: Choroid plexus ; Human fetus ; Modifications during development ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural organization of the human fetal choroid plexus was assessed with scanning electron microscopy. The membranous modifications of choroidal ependymal cells differ remarkably between 11 and 20 weeks of intrauterine development and suggest a variable functional capacity at different times of ontogenesis. Based upon existing data coupled with the ultra-architectural organization of cilia, clavate and linear microvilli are seen with scanning electron microscopy, a multiple functional role is hypothesized for choroidal ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220145
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  • 7
    Electronic Resource
    Electronic Resource
    The ventricular system in neuroendocrine mechanisms (1977)
    Springer
    Cell & tissue research 179 (1977), S. 235-254 
    Details
    ISSN: 1432-0878
    Keywords: Supraependymal axons-neurons ; Neurocisternal secretion ; Receptor cells ; Scanning-transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This investigation has utilized a correlative scanning-transmission electron microscopic technique in the analysis of the primate cerebral ventricular system. This approach has demonstrated a complex network of supraependymal cellular elements upon the walls of the third cerebral ventricle in direct contact with the ventricular lumen. Type I neuronal-like cells and type II histiocytic-like cells with potential phagocytic capabilities have been observed in large numbers throughout the third ventricle. Type I neuron-like cells are discussed in the context that they may represent a population of receptor-cells which serve to assess ambient changes in the composition of bioactive peptides in the cerebrospinal fluid and may serve as a supraependymal network that integrates the endocrine hypothalamus with other circumventricular organs which may also be sites of neuroendocrine transduction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219799
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  • 8
    Electronic Resource
    Electronic Resource
    A cluster of supraependymal neurons located within the infundibular recess of the rat third ventricleSupported in part by PHS grant 5429 and NSF INST grant 1972-141. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 140 (1974), S. 129-133 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A nidus of supraependymal cells was located within the infundibular recess of a rat's third ventricle. Upon transmission electron microscopic analysis, these cells were clearly identified as neurons. Neuronal processes, as well as end-terminals that contained dense-core and clear synaptic-like vesicles, were also observed coursing around these cells. It is suggested that some of the supraependymal cells that have been observed in previous scanning electron microscopic studies could be neurons similar to those described here.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001400109
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