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Digitale Medien

Sarcoplasmic Calcium-binding Proteins in Aplysia Nerve and Muscle Cells (1993)

Pauls, T. L. ; Cox, J. A. ; Heizmann, C. W. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

European journal of neuroscience 5 (1993), S. 0

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ISSN: 1460-9568

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Muscle (body wall, buccal mass, heart) and neural tissue of the marine mollusc Aplysia californica was analysed for calcium-binding proteins using transblot45Ca overlay, Western blotting and two-dimensional polyacrylamide gel electrophoresis, and several low molecular weight calcium-binding proteins were identified. Our results show that Aplysia muscle contains an abundant protein with a Mr of ∼20 000 with strong 45Ca2+-binding ability and cross-reactivity to antibodies against the sarcoplasmic calcium-binding protein isoform II (SCP II) from Amphioxus. Immunocytochemical studies revealed that isoforms of SCP are distributed in a tissue-specific manner, SCP II-like protein is exclusively present in muscle fibres closely associated with the contractile machinery, whereas the isoform I (SCP I-like protein) is exclusively present in a subset of neurons, suggesting a function in their calcium regulation. In addition, a novel 45Ca2+-binding protein of Mr 43 000, pl 4.7, was found in muscle and in neurons. A third protein of Mr 40 000, pl 4.8, cross-reacts with anti-parvalbumin and anti-calbindin D-28K antibodies.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1460-9568.1993.tb00520.x

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Digitale Medien

Calcium-binding proteins inAplysia neurons (1991)

Hermann, A. ; Pauls, T. L. ; Heizmann, C. W.

Springer

Cellular and molecular neurobiology 11 (1991), S. 371-386

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Details

ISSN: 1573-6830

Schlagwort(e): Ca-binding proteins ; parvalbumin ; calbindin D-28K ; sarcoplasmic calcium-binding protein ; electrical activity ; mollusk neurons ; Aplysia californica

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary 1. Calcium (Ca)-binding proteins of neuronal ganglia and of single, identified neurons of the marine mollusk,Aplysia californica, were investigated. Using transblot/45Ca overlays two proteins, atM r 45,000 andM r 23,000, with a high Ca-binding ability were found. 2. Western blot analysis revealed that the protein atM r 45,000 could be separated by 2D-PAGE into proteins withM r 40,000 andM r 43,000. The protein atM r 40,000 immunocross-reacted with antisera directed against parvalbumin and rat calbindin D-28K, indicating a novel Ca-binding protein sharing common antigenic determinants for both proteins. 3. The protein atM r 23,000 could be separated into a group of proteins withM r 13,000–20,000 which showed a high degree of similiarity to sarcoplasmatic calcium-binding proteins (SCP). 4. We further investigated the protein pattern of single, identified neurons of different electrical activity (bursting, beating, and silent) by 2D-PAGE. Major differences were found in the range of lowM r and lowpI, where Ca-binding proteins are generally located. A protein at high concentrations characteristic for silent cells migrated at a position similar to crayfish SCP. 5. The results show that various Ca-binding proteins are characteristic for neurons in theAplysia nervous system and support the idea that they may effect the electrical behavior of nerve cells.