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  • 1
    Electronic Resource
    Electronic Resource
    Mixed culture biooxidation of phenol. I. Determination of kinetic parameters (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 889-896 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A mixed culture derived from soil and activated sludge organisms was used to degrade phenol which was inhibitory to microorganisms at higher concentrations. The purpose of the experiments was to determine the kinetic parameters governing growth of the organisms by measuring growth rates in batch culture. To maintain a constant inoculum for the experiments inoculum was taken from a continuously operating continuous culture. Two populations were studied corresponding to two separate residence times in the continuous culture apparatus. One contained predominantly filamentous organisms, the other nonfilamentous. Five kinetic models were applied to the data and the best kinetic parameters for each model were determined by nonlinear least squares techniques. The models were then evaluated for best relative fit to the data. No significant differences were found between the models on the basis of fit and so a choice was made on the grounds of simplicity. A model proposed by Haldane was chosen as the best. No function however gave a satisfactory fit at the highest growth rates obtained. This experimental maximum in the plot of growth rate against substrate concentration was very sharp.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150506
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mixed culture biooxidation of phenol. III. Existence of multiple steady states in continuous culture with wall growth (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 905-916 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is shown that two steady states exist in certain regions of operation of a 2-liter continuous stirred tank biological reactor. Transition was made from one steady state to another by applying shock loads of either phenol substrate which is inhibitory to the culture at high concentrations or by adding large additional amounts of concentrated organisms. The existence of the multiple steady states is ascribed to the existence of wall growth, and their position is determined by the amount of wall growth. Transient behavior of the system did not follow the predictions of the simple wall growth model but the culture appeared to undergo a lag period immediately after applying the shock load to the system. It is concluded that the stability of a continuous culture utilizing an inhibitory substrate is improved by increasing the degree of wall growth and decreasing the substrate feed concentration. It is also concluded that small scale experiments can usually not be interpreted correctly unless the effect of wall growth is taken into account.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150508
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Effect of wall growth on scale-up problems and dynamic operating characteristics of the biological reactor (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 253-265 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: If inhibitory substrates are being utilized in a well-stirred biological reactor, microbiological growth on the walls of the reactor can create a scale-up problem. A simple model is proposed which shows that without such growth, of the three existing steady states only one is stable and nontrivial, but with wall growth the trivial, stable, steady state (washout) is impossible. In addition, wall growth reduces the region over which three steady states are feasible and reduces the minimum residence time for which there is only one steady state that corresponds to a high conversion. Thus, a laboratory process with a high surface area to volume ratio can give an over optimistic prediction of both necessary residence; time and stability of the full scale process unless wall growth is accounted for.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140208
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Mixed culture biooxidation of phenol. II. Steady state experiments in continuous culture (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 897-903 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Experiments are reported in which a mixed population of organisms was continuous cultured on phenol in a 1- to 2-liter well-mixed vessel. Steady state phenol concentrations were measured for a range of inlet concentrations from 100 to 800 mg/liter at various dilution rates. These results were compared with those predicted from a model which incorporates the effect of wall growth. It was found that the effect of growth on the walls of the vessel was considerable and increased by a factor of up to 3 × the dilution rate at which 90% conversion of phenol could be obtained.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150507
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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