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  • 1
    Electronic Resource
    Electronic Resource
    Lipid Forniation in Cryptococcus terricolus V. Oxidation of Fatty Acids by Cell Suspensions (1963)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 16 (1963), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1963.tb08299.x
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  • 2
    Electronic Resource
    Electronic Resource
    Light-Dark Transients in Levels of Intermediate Compounds during Photosynthesis in Air-Adapted Chlorella (1966)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 19 (1966), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The labeling of intermediate compounds and photosynthetic cofactors during photosynthesis and periods of darkness by Chlorella pyrenoidosa in the presence of 32P-labeled phosphate and 14CO2 have heen investigated. Algae adapted to photosynthesis in air were used, and the level of carbon dioxide was maintained at approximately 0.04 % and at constant specific radioactivity during the course of the experiments.The transient changes which occur in the levels of labeled fructose-1,6-diphosphate and in sedoheptulose-1,7-diphosphate, and in the corresponding monophosphates when the light is turned off suggest a light activation of the diphosphatase enzymes which decays after about 2 minutes of darkness. It is suggested that a light-dark switch in enzymic activities permits photosynthesis and glycolysis to occur in light and dark respectively with the same enzymic apparatus.The greatly diminished rate of disappearanec of the carboxylation substrate, ribulose-1,5-diphosphate, after about 2 minutes suggests that there is also a light activation of the carboxylation reaction in vivo. Large transient changes in the level of pyrophosphate between light and dark indicate that there may be an unstable cofactor which decomposes to give pyrophosphate during or alter killing of the algal cells. The possibility that this cofactor is involved in an activation of Carbon dioxide for the carboxylation reaction in vivo is suggested.Light-dark transient changes in labeling of other compounds of the photosynthetic carbon reduction cycle and related compounds were determined, and possible significance of these changes is discussed.(PDF DAMAGED)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1966.tb09094.x
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  • 3
    Electronic Resource
    Electronic Resource
    Lipid Formation in Cryptococcus terricolus VI. Effect of Malonate on Respiration (1963)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 16 (1963), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1963.tb08300.x
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on the Physiology of the Soil Yeast Cryptococcus terricolus (1960)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 13 (1960), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A new species of yeast, belonging to the genis Cryptococcus, and named Cryptococcus terricolus nov. spec., was recently isolated from different Norwegian forest soils (Pedersen 1958). Besides being of taxonomic interest, this yeast was found to produce lipids in great quantities. The presence of lipids could easily be estahlished by microscopical examination, owing to the formatinn of a large lipid globule in every cell. In the present paper, investigations on the growth conditions of the organism are presented. The factors influencing the lipid synthesis will be treated in a future article.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1960.tb08010.x
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  • 5
    Electronic Resource
    Electronic Resource
    Growth of Boletus variegatus in Surface and Shake Cultures (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A method of growing the mycelium of Boletus variegatus Fr. (Suillus variegatus Kuntze) in shake cultures is described. The growth curves of the fungus in stationary surface cultures and shaken submerged cultures have been compared. Growth in stationary cultures comprised (1) an acceleration phase, characterized by an approximately linear cube root plot, (2) a linear phase of constant increase of dry weight per day, and (3) a deceleration phase. In the shake cultures, two growth periods, both characterized by linear cube root plots, were observed. The growth rate constant was higher for the first than for the second period. During the second period, enlarged rounded cells with dense contents but normal cell walls were formed. The carbon source was exhausted and growth stopped after nine days in the agitated cultures and after about three weeks in the stationary ones. The economic coefficient for glucose utilization was higher in the agitated than in the stationary cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1970.tb08887.x
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  • 6
    Electronic Resource
    Electronic Resource
    Fatty Acid Induced Leaking of Organic Compounds from Boletus variegatus (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Addition of octanoic acid (2· 10-3M) to the suspending medium (final pH 4.85) of Boletus variegatus mycelium induced a marked leaking of UV absorbing substances from the cells. The material had an absorption maximum at 260 nm, a minimum at 240 nm, and the absorption ratios 250: 260 and 280:260 were 0.81 and 0.49.The material released immediately after addition of the acid consisted mainly of low molecular weight substances. These substances, listed according to decreasing rates of leaking, were identified as pentoses, pentosephosphates, nucleosides, and mono- and di-nucleotides. Also, purine and pyrimidine bases were released at this early stage of treatment.After 90 minutes' treatment, an outflux of oligoribonucleotides was observed. The oligoribonucleotides did not occur as single substances, but were forming complexes with peptides. Minor amounts of ribonucleic acid were also leaking out from the cells. Deoxyribose containing substances were never observed in the filtrates.The compounds were subjected to enzymatic degradation after they had left the cells. This was shown by a marked increase with time of inorganic phosphorus, pentose/pentosephosphates, and nucleosides in the filtrate.The leaking of low molecular weight substances immediately after acid addition is correlated to seriously reduced growth. However, the growth was wholly restored after a three days' lag period. On the other hand, when considerable amounts of oligoribonucleotide peptides had been released from the cells, growth could not be re-established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1970.tb06466.x
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of Fatty Acids and Methyl Octanoate on Resting Mycelium of Boletus variegatus (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The endogenous respiration of resting, submerged grown Boletus variegatus mycelium has been determined. In young cultures the intensity of the endogenous oxygen uptake was subject to great variations during the first few hours of starvation. However, by using six to eight days old mycelium the Qo2 values could be kept at a relatively low and constant level for at least nine hours.Inhibition of the endogenous respiration was found after addition of n-saturated C-2 to C-12 fatty acids (2 × 10-3M, pH 4.85). The inhibitory effect of the compound was dependent on the length of the carbon chain. Maximum effects were obtained for acids with eight to twelve carbon atoms per molecule. The inhibition was also dependent on the amount of undissociated acid present. By raising the pH so that the fatty acid dissociated the established inhibition was partly reversed. The effect of the neutral compound methyl octanoate was in essence identical to that obtained with octanoic acid.After fatty acid addition a close correspondence was found between the degree of inhibition of the oxygen uptake and the amount of UV absorbing substances leaking out from the cells. This extracellular material had an absorption maximum at 260 nm and a minimum around 240 nm. The leaking was ascribed to interaction between fatty acids or methyl octanoate and lipophilic substances of the cytoplasmic membrane. It is suggested that the inhibitory action on the endogenous respiration is due to similar effects on intracellular membrane systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1970.tb06458.x
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  • 8
    Electronic Resource
    Electronic Resource
    Interaction of 8-anilino-l-naphthalene sulfonate with fatty acid treated mycelium of Boletus variegatus Fr. (1971)
    Springer
    Archives of microbiology 77 (1971), S. 118-126 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fluorescent dye (8-anilino-1-naphthalene-sulphonic acid), which gives no fluorescence, or only a weak one, when added to submerged grown mycelium of the hymenomycete Boletus variegatus, is found to result in a marked increase of fluorescence if the organism is treated with fatty acids. The increased fluorescence is dependent on the presence of undissociated acid molecules as well as on the chain length of the acid. The effect is not due to the acidic character of the molecules, but is highly dependent on lipophilic properties of the substances. Consequently, the fatty acids introduce changes in the characteristics of the cytoplasmic membrane probably as a result of interactions with lipophilic parts of the membrane. The paper finally discusses the contribution of the present results to the elucidation of the influence mechanism of fatty acids in regard to leaking and respiration
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408604
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  • 9
    Electronic Resource
    Electronic Resource
    Factors affecting viable cell counts of freeze-driedCryptococcus terricolus cells (1965)
    Springer
    Antonie van Leeuwenhoek 31 (1965), S. 232-240 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Freeze-drying ofCryptococcus terricolus cells in distilled water resulted in a survival of only 0.1% of the cells. The viability could be increased to 16% by the use of a dextran-sucrose-sodium glutamate solution as suspending medium. For freeze-dried material with both low and high survival rates, and for five as well as for ten days old cultures, malt extract solution was the superior reconstitution medium. Less, but still distinct, protective action was found with a synthetic glucose-urea-salt solution. The viable cell counts of cells freeze-dried in dextran-sucrose-sodium glutamate solution were independent of the medium used for plating. When distilled water was used as a medium for freeze-drying, two to four times higher counts were obtained with malt extract agar than with synthetic glucose-urea-salt agar. Of the twenty different media tried for freeze-drying, sucrose solution gave the best protection. The viability was greatly influenced by the concentration used, maximum values being obtained when more than 10% of sucrose was added. The survival rate increased with the age of the cells until the fifth day, but was independent of the concentration of cells in the suspension. Under optimum conditions a survival rate of more than 80% was reached.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02045903
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