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  • 1
    Electronic Resource
    Electronic Resource
    CHOLINERGIC AND NON-CHOLINERGIC NERVE ENDINGS IN RAT BRAIN–I (1962)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 9 (1962), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1962.tb07489.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of collidine (2,4,6-trimethylpyridine) on rat pineal gland and vas deferens nerves (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 301-306 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In previous work of our laboratory it was demonstrated that collidine (2,4,6-trimethylpyridine) abolishes the core osmiophilia and chromaffin reaction from rat pineal gland and vas deferens nerves. This abolition was apparent when tissues were briefly incubated in collidine or when they were fixed in glutaraldehyde or osmium tetroxide using collidine as a buffer substance. These and other results strongly suggested that the histochemical effect of collidine was due to depletion of monoamines stored in the vesicles core. To examine this hypothesis we studied in this work the effect of collidine on tissues that have taken up tritiated noradrenaline. It was found that tritium was released very rapidly to the incubation medium when collidine was applied to fresh tissues. This effect was not observed with other commonly used buffers such as cacodylate or phosphate. It was also found that tritium release also occurred, although to a lesser extent, when tissues were fixed in glutaraldehyde or osmium tetroxide using collidine as a buffer, and this release was not significant when collidine was applied to previously fixed tissues. Paper chromatographic analysis showed that the radioactive compound(s) extracted from tissues by collidine corresponded to noradrenaline and/or closely related compounds. An abstract of this work was sent to the 17th Annual Meeting of the Society for Neuroscience, New Orleans, Nov 16–21, 1987. Tomsig J.L. and Pellegrino de Iraldi A. Abstract 369-11.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493156
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of collidine (2,4,6-trimethylpyridine) on the osmiophilia and chromaffin reaction in the synaptic vesicles of rat pineal nerves (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 21-25 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat pineal nerve endings contain a population of small and of large synaptic vesicles that are either electron lucent or have electron-dense cores. It has been reported that their osmiophilia is elminated when collidine buffer is used in the fixation procedure. We investigated this effect and found that osmium tetroxide and potassium dichromate reactivity were abolished when excised pineal glands were briefly incubated with collidine buffer before glutaraldehyde-cacodylate fixation. Such an effect was not observed when collidine was applied after fixation. Glands that had been fixed in glutaraldehyde or osmium tetroxide buffered with collidine exhibited a peripheral zone containing reactive synaptic vesicles and a deeper, central zone where such reactivity was absent. These results indicate that the effect of collidine is due to depletion of monoamines rather than to chemical blockage of their reactivity, and further suggest that collidine has a higher rate of penetration into tissues than the tested fixatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00518720
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of pyridine and its methylated derivatives on storage by rat pineal nerves of monoaminergic neurotransmitter (1994)
    Springer
    Histochemistry and cell biology 101 (1994), S. 419-425 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In previous work of our laboratory, it was demonstrated that collidine (2-4-6-trimethylpyridine) applied briefly to fresh tissues extracted noradrenaline or closely related compound/s of neuronal origin. This effect gave rise to the abolition of osmiophilia and chromaffin reaction of electron-dense cores of monoaminergic synaptic vesicles and to the extraction of radioactive compounds in tissues that had previously taken up tritiated noradrenaline. In this work, the role of the pyridine ring and its progressive methylation on the monoamine releasing effect was investigated. For this purpose, the effect of pyridine, two picolines (2 and 4 monomethylpyridines) and a lutidine (2-6-dimethylpyridine) was compared to the effect of collidine. It was found that pyridine has a much smaller effect than collidine on the histochemical reactivity of monoaminergic synaptic vesicles and on the extraction of tritiated compounds and that its extent was dependent upon the number of methyl groups incorporated in the pyridine ring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269492
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  • 5
    Electronic Resource
    Electronic Resource
    A silver-reducing component in rat striated muscle (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 15-22 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study reports the presence of a silver-reducing constituent in rat striated muscle fiber located selectively at the level of the terminal cistern/transverse tubulc system. It is related to the T tubule network at or near sites that participate in junctions with terminal cisternae, i.e., at both sides of the T tubule in skeletal muscle (triad) and, predominantly, at one side in the ventricle (dyad). Little reactivity is present in the auricle due to the scarcity of those membrane systems. The longitudinal sarcoplasmic reticulum, the sarcolemma, mitochondria and myofibrils are not outlined by the reaction product. Extraction of low molecular weight substances, nucleic acids and lipids did not suppress the chemical reaction. A new argentaffin (Hg−Ag) technique is described. Ethanol or aldehyde fixed muscles were passed to water, posfixed 6–24h with mercuric acetate (5% w/v in 1% acetic acid), washed with 1% acetic acid and distilled water, stained 12–24 h at 43° C with ammoniacal silver nitrate (60% w/v) and washed in 10% sodium sulfite (three changes) and water. All steps were carried out in darkness. Postfixation with mercuric acetate proved to be essential for immobilizing the argentaffin component without interfering with its strong argentaffinity. The procedure also provides a simple method for tracing the pathway of transversally oriented membrane systems in skeletal and cardiac muscle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495010
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  • 6
    Electronic Resource
    Electronic Resource
    A silver-reducing component in rat striated muscle (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 23-27 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In previous work on rat striated muscle cells a sliver-reducing component was found selectively localized at the terminal cistern/transverse tubule system (Tandler and Pellegrino de Iraldi 1989). To further investigate that problem we performed the Hg−Ag argentaffin reaction on a sarcoplasmic reticulum fraction from rat skeletal muscle. Circular profiles corresponding to vesicular structures were found outlined by silver grains. The number of silver “stained” vesicles were less than the total number vesicles stained by conventional procedures. The correlation between argentaffinities in the intact muscle fiber and their subcellular organelles indicated that the Hg−Ag reactive vesicles must be those derived from the terminal cisternae of the sarcoplasmic reticulum. The silver-reducing constituent aggregates in the presence of 1 mM CaCl2 or 0.5 M K cacodylate. The state of aggregation induced by Ca2+ was not affected by incubation with 0.5% Triton X-100 or by 2 mM EDTA, thus suggesting a localization at or near the membrane of the terminal cistern vesicle facing the junctional gap. In Laemmli SDS-acrylamide gels the Hg−Ag reaction stained all proteins in a manner similar to Coomasie blue. It is suggested that the selective histochemical staining is the result of differential reactivities due to steric requirements of the chemical reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495011
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