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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of a yeast gene “in vivo” and its transfer as a part of an autonomously replicating unit: gene seduction (1985)
    Springer
    Current genetics 9 (1985), S. 119-121 
    Details
    ISSN: 1432-0983
    Keywords: Episomal plasmid ; Chromosomal integration ; Plasmid rescue ; Gene seduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Yeast diploids containing a chromosomally integrated episomal plasmid manifest the mitotic instability of the chromosomes with the plasmid. Probably as a results of the destabilization integrated plasmid may be excised out of the chromosome. It was found that the rescue of the plasmid may be irregular, with the taking up of adjacent chromosomal gene. Using an integrant with the plasmid integrated into chromosome I very near the ADE1 locus we cloned this gene “in vivo” by selecting rescued plasmid marker LEU2. The new plasmid has retained the LEU2 gene and the capacity to replicate autonomously. This plasmid was used to transfer the cloned ADEI gene from one cell to another by transformation and from one resident chromosome to another by integration. The phenomenon of irregular excision of an integrated episomal plasmid together with linked chromosomal gene(s) and transfer to other chromosomes or cells we propose to term Seduction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436958
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  • 2
    Electronic Resource
    Electronic Resource
    The start gene CDC28 and the genetic stability of yeast (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 6 (1990), S. 231-243 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; cell cycle regulation ; CDC28 gene ; rho- mutation ; chromosome loss ; plasmid maintenance ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The cdc28-srm mutation in Saccheromyces cerevisiae decreases spontaneous and induced mitochondrial rhomutability and the mitotic stability of native chromosomes and recombinant circular minichromosomes. The effects of cdc28-srm on the genetic stability of cells support the hypothesis that links cell cycle regulation in yeast to changes in chromatin organization dependent on the start gene CDC28 (Hayles and Nurse, 1986).
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320060308
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    Paper (German National Licenses)
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