ISSN:
1432-1041
Keywords:
Dextromethorphan
;
ELISA
;
polymorphism
;
population study
;
French Caucasians
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Medicine
Notes:
Summary Simple, sensitive and selective enzyme immunoassays (ELISA) for monitoring urinary dextromethorphan and its major metabolite, dextrorphan, were developed. Dextromethorphan and dextrorphan hemisuccinates were linked to bovine serum albumin and specific antisera against each immunogen were raised in rabbits. The sensitivity of the ELISA was high (limit of detection 740 and 600 pg·ml−1 for dextromethorphan and dextrorphan, respectively). Intra- and interassay variation was 〈10%, and cross-reactivity between the two compounds was 〈1%. The ELISA was employed to phenotype 216 French subjects. The frequency of poor metabolizers was 5.1%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00280940
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