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  • 1
    Electronic Resource
    Electronic Resource
    Genetically engineered plants producing opines alter their biological environment (1997)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 15 (1997), S. 369-372 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0497-369
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation and Regeneration of a Nitrogen-Fixing Tree, Allocasuarina Verticillata Lam. (1991)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 9 (1991), S. 461-466 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Hairy root formation was induced by inoculating epicotyl, cotyledon and hypocotyl of Allocasuarina verticillata with two strains of Agrobacterium rhizogenes, A4 (agropine type) or 2659 (cucumopine type). Shoot regeneration from hairy roots induced by strain A4, and from control roots excised from ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0591-461
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  • 3
    Electronic Resource
    Electronic Resource
    Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells (1982)
    [s.l.] : Nature Publishing Group
    Nature 295 (1982), S. 432-434 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A. rhizogenes strain 8196 (from Dr J. Lippincott) contains three plasmids, the intermediate-sized one being the virulence plasmid, isolated in transconjugant C58C1 (pRi 8196) (Fig. 1). Similar data have been reported by White and Nester7 for A. rhizogenes 15834. As expected, plasmid DNA isolated29 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/295432a0
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  • 4
    Electronic Resource
    Electronic Resource
    N-hexanoyl-l-homoserine lactone, a mediator of bacterial quorum-sensing regulation, exhibits plant-dependent stability and may be inactivated by germinating Lotus corniculatus seedlings (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 52 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The half-life of N-hexanoyl-l-homoserine lactone (C6-HSL) was determined under various pH and temperature conditions, and in several plant environments. C6-HSL was sensitive to alkaline pH, a process that was also temperature-dependent. In addition, C6-HSL disappeared from plant environments, i.e. axenic monocot and dicot plants cultivated under gnotobiotic, hydroponic conditions, albeit with variable kinetics. The disappearance was rapid at the root system of legume plants such as clover or Lotus, and slow or non-existent at the root system of monocots such as wheat or corn. These variable kinetics were not dependent upon pH changes that may have affected the growth media of the plants. Furthermore, C6-HSL did not accumulate in the plant, and the plant did not produce inhibitors of the C6-HSL signal. HPLC analyses revealed that C6-HSL disappeared from the media, and hence, Lotus exhibited a natural C6-HSL inactivating ability. This ability was not specific for C6-HSL and allowed the degradation of other N-acyl-homoserine lactones such as 3-oxo-C6-HSL, 3-oxo-octanoyl-HSL and 3-oxo-decanoyl-HSL. Preliminary investigation revealed that the inactivating ability is temperature-dependant and possibly of enzymatic origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.10.005
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  • 5
    Electronic Resource
    Electronic Resource
    T-DNA length variability in mannopine hairy root: more than 50 kilobasepairs of pRi T-DNA can integrate in plant cells (1988)
    Springer
    Plant cell reports 7 (1988), S. 92-95 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences covering more than 50 kbp of the Ri plasmid
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270112
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation of Agrobacterium Ti-plasmid regulatory mutants (1978)
    Springer
    Molecular genetics and genomics 167 (1978), S. 147-155 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crown gall tumors incited by Agrobacterium tumefaciens synthesize basic amino acid derivatives called opines. Opine production in tumours and opine catabolism by A. tumefaciens are coded by Ti-plasmids which confer oncogenicity on this bacterium. Catabolism of opines is inducible, and a method for isolation of regulatory mutants is described. From octopine-type bacteria, by plating on non-inducing substrates (noroctopine, noroctopine acid, D-histopine) we have isolated regulatory mutants of three types: constitutive, partially constitutive, and fully inducible by the analogue. From nopaline-type bacteria, by plating on octopine (a non inducing substrate) we have isolated analogous regulatory mutants. Synthetic opines, in which the amino acid moiety has been replaced by toxic arginine analogues, are toxic for these regulatory mutants. We isolated mutants resistant to such synthetic opines, and found that some had lost the capacity to utilize octopine. A survey of a large number of such mutants revealed that all of them still incited octopine synthetizing tumors. Mutants constitutive for octopine catabolism are in some instances also constitutive for Ti-plasmid transfer. A simple method for screening regulatory mutants for constitutive Ti-plasmid transfer is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266908
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  • 7
    Electronic Resource
    Electronic Resource
    Further extension of the opine concept: Plasmids in Agrobacterium rhizogenes cooperate for opine degradation (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 204-214 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Primary hairy root tissues as well as aseptic hairy root culture lines contain specific compounds that have been biologically characterized as opines. These substances are agropine, mannopine, mannopinic acid, and agropinic acid; they have been synthesized and their electrophoretic behavior has been studied. Hairy root tissues also contain agrocinopines. According to the opine content of hairy root tissues, two types of Agrobacterium rhizogenes strains have been identified. Agropine-type strains (A4, 15834, HRI) elicit roots containing agropine, mannopine, mannopinic acid, and agropinic acid, whereas mannopine-type strains (8196, TR7, TR101) elicit roots containing only mannopine, mannopinic acid and agropinic acid. A. rhizogenes strains catabolize the opines whose synthesis they induce in the hairy root tissues. However, strain HRI only catabolizes agropine. Except for strain HRI, all A. rhizogenes strains studied contain three plasmids, of which the largest appears to be a cointegrate of the two others. Transconjugants of A. rhizogenes plasmids in A. tumefaciens have been obtained by selection on opines. Their properties have been studied and related to their plasmid content. In the mannopine strain C58C1(pRi8196), the virulence functions and the opine-related functions are located on the same plasmid (pRi8196). In agropine strains the catabolic functions are dissociated: agropine degradation is specified by the virulence plasmid, which also specifies opine synthesis in hairy root tissue, however, mannopine, mannopinic acid and agropinic acid degradation are specified by the smaller plasmid. Strain HRI contains only the virulence plasmid, which explains its inability to degrade mannopine, mannopinic acid, and agropinic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330641
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  • 8
    Electronic Resource
    Electronic Resource
    Multiple transformation of plant cells by Agrobacterium may be responsible for the complex organization of T-DNA in crown gall and hairy root (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 388-393 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Ti plasmid ; Ri plasmid ; Opines ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inoculation of carrot discs and Lotus corniculatus plantlets with mixtures of different Agrobacterium rhizogenes or of A. rhizogenes and A. tumefaciens or with Agrobacterium strains harboring both an Ri and a modified Ti plasmid resulted in frequent multiple (pluribacterial) transformation of cells, as revealed by the mixed opine-type of hairy roots arising from them. Multiple transformation may account for the presence of dispersed T-DNA inserts in crown gall and hairy root lines. A plant genetic engineering strategy based on segregation of T-DNA inserts in the progeny of multiple transformants is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333267
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  • 9
    Electronic Resource
    Electronic Resource
    Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 245-250 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium rhizogenes ; Plant transformation ; Transgenic legumes ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure for transformation and regeneration of the legume species Lotus corniculatus (Bird's-foot trefoil) has been developed. The Agrobacterium rhizogenes 15834 and 8196 strains were used to transform plant cells in wound site infections and transformed roots were propagated in vitro. Transformation was monitored by hybridization with pRi T-DNA sequences and by detection of agropine and mannopine. Transformation frequencies of up to 90% were obtained. Shoots spontaneously formed on hairy root cultures were excised, rooted and inoculated with Rhizobium. Root nodules formed on transformed plants had nitrogenase activities comparable to untransformed nodules. Transcript levels from the nodule-specific leghemoglobin genes and the constitutive ubiquitin genes were similar in transformed and untransformed root nodules. Transformed plants responded to R. loti and Bradyrhizobium sp. (Lotus) strains with phenotypes identical to phenotypes for untransformed plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331585
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  • 10
    Electronic Resource
    Electronic Resource
    Arginine catabolism: A new function of both octopine and nopaline Ti-plasmids of Agrobacterium (1979)
    Springer
    Molecular genetics and genomics 173 (1979), S. 263-269 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The oncogenic plasmids of Agrobacterium, the Ti-plasmids, carry genes that enable their bacterial host to catabolize opines. Opines are unusual amino acid derivatives that are only produced in crown gall tumours incited by oncogenic strains of Agrobacterium. The 2 opines, octopine and nopaline, are degraded by Agrobacterium strains carrying the octopine or the nopoline Ti-plasmid, respectively, to arginine and pyruvic acid, and to arginine and α-ketoglutaric acid. In this paper it is shown that the Ti-plasmids carry gene(s) involved in the utilisation of arginine as a carbon source. Strains harbouring wild type octopine or nopaline Ti-plasmids in the chromosomal context of strain C58C1 do not grow on arginine as a carbon source. However, they are able to grow on arginine provided that they are induced, or constitutive for opine catabolism. The features of ornithine utilisation are identical. The gene(s) involved in arginine and ornithine utilization in C58C1 (pTi-oct) or C58C1 (pTi-nop) are under the control of the regulator gene that controls octopine or nopaline catabolism. A tentative pathway of octopine utilization is proposed, in which at least two steps are Ti-plasmid coded, and probably belong to the same operon: 1-scission of octopine into arginine and pyruvic acid 2-transformation of an arginine derivative (GSA?) to glutamic acid. Arginine utilization as a carbon source is therefore a new function of the Ti-plasmid. As this function is not inducible by arginine but by opines, it provides a method for selecting regulatory mutants of opine catabolism in the genetic background of strain C58.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268636
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