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  • 1
    Electronic Resource
    Electronic Resource
    The chimpanzee major histocompatibility complex class II DR subregion contains an unexpectedly high number of beta-chain genes (1990)
    Springer
    Immunogenetics 32 (1990), S. 272-280 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The major histocompatibility complex (MHC) class II DR subregion of the chimpanzee was studied by restriction fragment length polymorphism (RFLP) analysis. Genomic DNA obtained from a panel of 94 chimpanzees was digested with the restriction enzyme Taq I and hybridized with an HLA-DRβ probe specific for the 3' untranslated (UT) region. Such a screening revealed the existence of 14 distinct DRB/Taq I gene-associated fragments allowing the definition of 11 haplotypes. Segregation studies proved that the number of chimpanzee class II DRB/Taq I fragments is not constant and varies from three to six depending on the haplotype. Comparison of these data with a human reference panel manifested that some MHC DRB/Taq I fragments are shared by man and chimpanzee. Moreover, the number of HLA-DRB/Taq I gene-associated fragments detected in a panel of homozygous typing cells varies from one to three and corresponds with the number of HLA-DRB genes present for most haplotypes. However, a discrepancy is observed for the HLA-DR4,-DR7, and -DR9 haplotypes since a fourth HLA-DRB pseudogene present within these haplotypes lacks its 3' UT region and thus is not detected with the probe used. These results suggest that chimpanzees have a higher maximum number of DRB genes per haplotype than man. As a consequence, some chimpanzee haplotypes must show a dissimilar organization of the MHC DR subregion compared to their human equivalents. The implications of these findings are discussed in the context of the trans-species theory of MHC polymorphism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187098
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  • 2
    Electronic Resource
    Electronic Resource
    The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds (1996)
    Springer
    Plant molecular biology 32 (1996), S. 515-523 
    Details
    ISSN: 1573-5028
    Keywords: pea lectin ; promoter analysis ; seed-specific element ; tissue-specific expression ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5′ promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit at a low level. This fragment contains a 22 bp element (W1), which has been demonstrated to be important for seed-specific expression when coupled as a trimer to a heterologous TATA box (de Pater et al., Plant Cell 5: 877–886, 1993). Here we show that deletion of W1 in the natural promoter context resulted in a strongly decreased level of gene expression. A 4 bp mutation of W1 reduced the expression of truncated derivatives of the Psl promoter. A single copy of W1 coupled to the TATA box of the CaMV 35S promoter directed low gene expression in seeds and leaves. Multimerization enhanced the expression in seeds up to 100-fold, to levels found with the Psl promoter, whereas the expression level in leaves remained low. These results demonstrate that the W1 element is an essential control element in the Psl promoter. When taken out of its natural context and multimerized, it is sufficient for high expression in seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019103
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    RAP-1 is an Arabidopsis MYC-like R protein homologue, that binds to G-box sequence motifs (1997)
    Springer
    Plant molecular biology 34 (1997), S. 169-174 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; bHLH domain ; DNA binding ; MYC-like protein ; R gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An Arabidopsis cDNA clone encoding a DNA-binding protein, RAP-1, was isolated by southwestern screening of an Escherichia coli cDNA expression library. The protein contains a bHLH DNA-binding domain and is homologous to R proteins, regulating anthocyanin biosynthesis. RAP-1 binds to the sequence CACNTG. It is encoded by a single gene, which is expressed to high levels in root and stem and to low levels in leaf and flower. No expression could be detected in siliques. Rap-1 does not correspond to one of the known loci involved in anthocyanin biosynthesis, since it is located at a different map position. In contrast to the maize R protein Lc, RAP-1 did not induce anthocyanin biosynthesis in pea cotyledons. Thus, RAP-1 is a novel member of the bHLH class of DNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005898823105
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  • 4
    Electronic Resource
    Electronic Resource
    Binding specificity and tissue-specific expression pattern of theArabidopsis bZIP transcription factor TGA2 (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 237-239 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; bZIP domain ; C-box binding protein ; TGA family ; Transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The binding specificity and tissue-specific expression pattern of TGA2 (AHBP-1b), anArabidopsis bZIP transcription factor have been determined. Filter-binding and gel-shift assays showed that TGA2 has high affinity for C-boxes (ATGACGTCAT). In this respect TGA2 is similar to other members of theArabidopsis TGA family (such as TGA1, TGA3 and OBF4) and to tobacco TGA1a. Genomic Southern blot analysis confirmed thatTGA2 is a member of a gene family. Northern blot analysis showed that the gene is expressed at similar levels in root, stem, leaf and flower and at somewhat lower levels in siliques.TGA3 was also found to be expressed at the same level throughout the plant, whereas genes encoding TGA1 and OBF4 have relatively high RNA expression levels in root. The differential expression of these genes suggests that they have distinct functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174184
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  • 5
    Electronic Resource
    Electronic Resource
    Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2 (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 237-239 
    Details
    ISSN: 1617-4623
    Keywords: Key words Arabidopsis thaliana ; bZIP domain ; C-box binding protein ; TGA family ; Transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The binding specificity and tissue-specific expression pattern of TGA2 (AHBP-1b), an Arabidopsis bZIP transcription factor have been determined. Filter-binding and gel-shift assays showed that TGA2 has high affinity for C-boxes (ATGACGTCAT). In this respect TGA2 is similar to other members of the Arabidopsis TGA family (such as TGA1, TGA3 and OBF4) and to tobacco TGA1a. Genomic Southern blot analysis confirmed that TGA2 is a member of a gene family. Northern blot analysis showed that the gene is expressed at similar levels in root, stem, leaf and flower and at somewhat lower levels in siliques. TGA3 was also found to be expressed at the same level throughout the plant, whereas genes encoding TGA1 and OBF4 have relatively high RNA expression levels in root. The differential expression of these genes suggests that they have distinct functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174184
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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