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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 99 (1997), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Degenerate oligonucleotide primers based on the amino acid sequences of gibberellin (GA) 20-oxidases from pumpkin and Arabidopsis were used in nested polymerase chain reactions to generate an internal cDNA fragment from rice (Oryza satiya L.) seedlings. Using this fragment as a probe, a full-length cDNA (pOs20ox) was isolated from a cDNA library constructed from rice seedlings. The deduced amino acid sequence of Os20ox showed good identity (42–55%) with the GA 20-oxidases from pumpkin and Arabidopsis. Recombinant Os20ox protein, produced in Escherichia coli, catalyzed the conversion of GA12 and GA53 to GA9 and GA20, respectively. In contrast to the enzyme from immature pumpkin seeds, the recombinant rice GA 20-oxidase did not produce the tricarboxylic acids GA25 and GA17 from GA12 and GA53, respectively. The conversion rate of GA53 was higher than that of GA12. which is consistent with the relatively high abundance of 13-hydroxylated GAs in rice seedlings. Os20ox mRNA accumulated at higher levels in seedlings of two GA-deficient dwarf mutants than in normal plants. Treatment with uniconazole-P. an inhibitor of GA biosynthesis, increased abundance of the mRNA, while exogenously applied GA3 decreased it. These results suggest that the expression of the Os20ox gene is regulated by the level of physiologically active GA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1997.tb03438.x
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  • 2
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    Unknown
    CHRISTMAS EVE (1943)
    Washington, etc. : Periodicals Archive Online (PAO)
    Poet lore. 49:4 (1943:Winter) 355 
    Details
    ISSN: 0032-1966
    Topics: English, American Studies
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:3208-1943-049-04-000012
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A point mutation in the N-terminus of ribulose-1,5-bisphosphate carboxylase affects ribulose-1,5-bisphosphate binding (1991)
    Springer
    Planta 184 (1991), S. 35-39 
    Details
    ISSN: 1432-2048
    Keywords: Ribulose ; 1,5-bisphosphate carboxylase ; Gene mutation (N-terminus) ; Substrate affinity (ribulose-1,5-bisphosphate)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutagenesis in vitro of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) from Anacystis nidulans was used to generate novel enzymes. Two conserved residues, threonine 4 and lysine 11 in the N-terminus were changed. The substitution of threonine 4 with serine or valine had little effect on the kinetic parameters. The substitution of lysine 11 with leucine, which is non-polar, increased the K m for ribulose-1,5-bisphosphate from 82 to 190 μM but its replacement with glutamine, which has polar properties, had no appreciable effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208233
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  • 4
    Electronic Resource
    Electronic Resource
    Localisation of cis elements in the promoter of a wheat α-Amy2 gene (1992)
    Springer
    Plant molecular biology 19 (1992), S. 903-911 
    Details
    ISSN: 1573-5028
    Keywords: α-amylase gene expression ; aleurone protoplasts ; functional analysis ; cis elements ; hormone regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A functional analysis of the promoter from the wheat α-amylase gene α-Amy2/54 is described. Mutant α-Amy2/54 promoters containing replacements or deletions were constructed and their ability to direct expression of the reporter gene β-glucuronidase (GUS) in gibberellin-responsive oat aleurone protoplasts analysed. Chimaeric promoters using regions of the cauliflower mosaic virus (CaMV) 35S and α-Amy2/54 promoters were also analysed. The results suggest that at least three regions within the α-Amy2/54 promoter contain cis elements that are necessary for high-level gibberellin-regulated transcription. Fusion of 1.8 kb of promoter sequence upstream from −117 bp to a minimal (−55 CaMV 35S) promoter gave rise to hormone-independent expression implying that the region 3′ to −117 bp contains an element which represses transcription in the absence of gibberellin or presence of abscisic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040523
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    Paper (German National Licenses)
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