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  • 1
    Electronic Resource
    Electronic Resource
    Catabolite control of the elevation of PGK mRNA levels by heat shock in Saccharomyces cerevisiae (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Heat shock enhances the very high level of transcription of the phosphoglycerate kinase (PGK) gene in fermentative cultures of Saccharomyces cerevisiae. This response of PGK mRNA levels was not found on gluconeogenic carbon sources, and could be switched on or off subject to availability of fermentable carbon source. The addition of glucose to yeast growing on glycerol resulted in acquisition, within 30–60 min, of the ability to elevate PGK mRNA levels after heat shock. In addition, in aerobic cultures growing on glucose the exhaustion of the medium glucose coincided with a loss of the heat-shock effect on PGK mRNA and a switch-over to slower growth by aerobic respiration. Levels of hsp26 mRNA were analysed during these experiments.Contrasting with this requirement for fermentable catabolite for manifestation of a heat-shock response of PGK mRNA levels, the PGK enzyme was not synthesized at a greater level in heat-shocked fermentative than in gluconeogenic cultures. PGK is one of only a few proteins made efficiently after mild heat shock of yeast. Thus, heat-stress-induced elevation of PGK mRNA levels does not appreciably increase PGK synthesis during exposure to high temperatures and so its role may be to assist cells repressed in mitochondrial function during recovery following a heat shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00039.x
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus (1998)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 671-674 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Recombinant Sulfolobus solfataricus glyceraldehyde-3-phosphate dehydrogenase has been purified and found to be a tetramer of 148 kDa. The enzyme shows dual cofactor specificity and uses NADP+ in preference to NAD+. The sequence has been compared with other GAPDH proteins including those from other archaeal sources. The purified protein has been crystallized from ammonium sulfate to produce crystals that diffract to 2.4 Å with a space group of P43212 or P41212. A native data set has been collected to 2.4 Å using synchrotron radiation and cryocooling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444997018076
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  • 3
    Electronic Resource
    Electronic Resource
    Only one of two closely related yeast suppressor tRNA genes contains an intervening sequence (1981)
    [s.l.] : Nature Publishing Group
    Nature 291 (1981), S. 464-469 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The yeast genes that code for the serine-inserting SUP-RL1 amber and SUQ5 ochre suppressors have been cloned and sequenced. These two unlinked genes differ by only three base pairs in their coding regions yet they encode tRNAs of different translational specificities, and while the SUP-RL1 gene has ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/291464a0
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  • 4
    Electronic Resource
    Electronic Resource
    Growth rate influences MFα1 promoter activity in MATαSaccharomyces cerevisiae (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 340-345 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The signal sequences of the MFα1 prepro α-factor gene are frequently used to direct secretion of heterologous proteins from Saccharomyces cerevisiae. They are often employed together with the MFα1 promoter in secretion vectors, such that this promoter directs the transcription of many heterologous gene cassettes in yeast. Most of the existing literature indicates that the MFα1 promoter is constitutive in MATα cells, although some data suggests that it may be more active in respiratory or late logarithmic fermentative cultures. To identify whether there is a growth rate or medium control over MFα1 promoter activity a strain was constructed with an integrated MFα1 promoter-β-galactosidase (lacZ) reporter gene fusion. Intracellular β-galactosidase of this strain during batch culture on glucose, raffinose and acetate showed that MFα1 promoter activity was higher during respiratory growth on acetate as compared to more rapid fermentative growth on glucose or raffinose, a result that might indicate this activity being inversely related to growth rate. Chemostat culture confirmed that growth rate does indeed influence MFα1 promoter activity in glucose-grown cells, the activity of this promoter increasing 2- to 2.5-fold as dilution (growth) rates were reduced from maximal values to 0.2 h-1, but then decreasing with the further decreases in dilution rate needed for fully respiratory growth. Thus a promoter generally thought to be constitutive in MATα cells is nevertheless subject to a complex growth rate control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902739
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  • 5
    Electronic Resource
    Electronic Resource
    Growth rate influences MFα1 promoter activity in MATαSaccharomyces cerevisiae (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 340-345 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The signal sequences of the MFα1 prepro α-factor gene are frequently used to direct secretion of heterologous proteins fromSaccharomyces cerevisiae. They are often employed together with the MFα1 promoter in secretion vectors, such that this promoter directs the transcription of many heterologous gene cassettes in yeast. Most of the existing literature indicates that the MFα1 promoter is constitutive in MATα cells, although some data suggests that it may be more active in respiratory or late logarithmic fermentative cultures. To identify whether there is a growth rate or medium control over MFα1 promoter activity a strain was constructed with an integrated MFα1 promoter β-galactosidase (lacZ) reporter gene fusion. Intracellular β-galactosidase of this strain during batch culture on glucose, raffinose and acetate showed that MFα1 promoter activity was higher during respiratory growth on acetate as compared to more rapid fermentative growth on glucose or raffinose a result that might indicate this activity being inversely related to growth rate. Chemostat culture confirmed that growth rate does indeed influence MFα1 promoter activity in glucose-grown cells, the activity of this promoter increasing 2- to 2.5-fold as dilution (growth) rates were reduced from maximal values to 0.2 h−1, but then decreasing with the further decreases in dilution rate needed for fully respiratory growth. Thus a promoter generally thought to be constitutive in MATα cells is nevertheless subject to a complex growth rate control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902739
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  • 6
    Electronic Resource
    Electronic Resource
    Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic Archaeon Sulfolobus solfataricus (1996)
    Springer
    Current genetics 29 (1996), S. 594-596 
    Details
    ISSN: 1432-0983
    Keywords: Key words Yeast ; Heterologous gene expression ; Sulfolobus ; Hyperthermophile phosphoglycerate kinase ; Archaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The gene encoding phosphoglycerate kinase (PGK) from the Archaeon Sulfolobus solfataricus, an organism growing optimally at 87 °C, was inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector was then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37 °C, but not 28 °C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enabled complementation of the yeast pgk defect at 37 °C but not at 28 °C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050091
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  • 7
    Electronic Resource
    Electronic Resource
    Polyubiquitin gene expression contributes to oxidative stress resistance in respiratory yeast (Saccharomyces cerevisiae) (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 358-362 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Oxidative stress ; High temperature viability ; Ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract UBI4, the polyubiquitin gene of Saccharomyces cerevisiae, is expressed at a low level in vegetative cells, yet induced strongly in response to starvation, cadmium, DNA-damaging agents and heat shock. UBI4 is also expressed at a higher basal level in cells growing by respiration as compared to glucose-repressed cells growing by fermentation. This higher UBI4 expression of respiratory cultures probably helps to counteract the greater oxidative stress of respiratory growth. The effects of inactivating UBI4 on high temperature viability are more marked with respiratory cultures. Also loss of UBI4 leads to a considerably increased rate of killing of respiring cells by hydrogen peroxide, whereas the same gene inactivation has relatively little effect on the peroxide sensitivity of cells in which mitochondrial functions are repressed. This is the first study to reveal that ubiquitin levels in cells can influence their ability to withstand oxidative stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301072
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  • 8
    Electronic Resource
    Electronic Resource
    UBI4, the polyubiquitin gene of Saccharomyces cerevisiae, is a heat shock gene that is also subject to catabolite derepression control (1997)
    Springer
    Molecular genetics and genomics 253 (1997), S. 439-447 
    Details
    ISSN: 1617-4623
    Keywords: Key words Saccharomyces cerevisiae ; Polyubiquitin gene ; Catabolite derepression ; Oxidative stress survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Carbon and nitrogen regulation of UBI4, the stress-inducible polyubiquitin gene of Saccharomyces cerevisiae, was investigated using a UBI4 promoter-LacZ fusion gene (UBI4-LacZ). Expression of this gene in cells grown on different media indicated that the UBI4 promoter is more active during growth on respiratory than on fermentable carbon sources but is not subject to appreciable control by nitrogen catabolite repression. UBI4-LacZ expression was virtually identical in cells having constitutively high (ras2, sra1-13) or constitutively low (ras2) levels of cyclic AMP-dependent protein kinase activity, indicating that this kinase does not exert a major influence on UBI4 expression. Catabolite derepression control of the UBI4 promoter was confirmed by measurements of UBI4-LacZ expression in hap mutant and wild-type strains before and after transfer from glucose to lactate. Mutagenesis of the perfect consensus for HAP2/3/4 complex binding at position −542 resulted in considerable reduction of UBI4 promoter derepression with respiratory adaptation in HAP wild-type cells and abolished the reduced UBI4-LacZ derepression normally seen when aerobic cultures of the hap1 mutant are transferred from glucose to lactate. This HAP2/3/4 binding site is therefore a major element contributing to catabolite derepression of the UBI4 promoter, although data obtained with hap1 mutant cells indicated that HAP1 also contributes to this derepression. The HAP2/3/4 and HAP1 systems are normally found to activate genes for mitochondrial (respiratory) functions. Their involvement in mediating higher activity of the UBI4 promoter during respiratory growth may reflect the contribution of UBI4 expression to tolerance of oxidative stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050341
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