ISSN:
1398-9995
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
The aim was to compare IgE and IgG4, RAST-inhibition assay (RI), monoclonal antibody ELISA (Mab-ELISA), counter current immuno electrophoresis (CCIE) and histamine release from basophil leukocytes (HR) for allegen quantification with special reference to aeroallergen detection. As components of indoor acroallergens, cat, dog, and Derm. pter. allergen extracts were selected for the experiments. To evaluate unspecific interference, these allergens were compared mutually and with Cladosporium herbarum. Allergen extracts in varying dilutions were mixed with crushed glass fibre filter materials, eluted, recovered by centrifugation, and allergen concentration quantified by the assays. Equal sensitivity was found for both IgE- and IgG4-RI assaying eat allergen (in the range 5 − 50 SQ-U/ml) and dog allergen (in the range 102− 103 SQ-U/ml). The IgG4-RI assaying Derm. pter, was more sensitive (50 SQ-U/ml) than IgE-RI (2*103 SQ-U/ml). The ranges of allergen detection limits for the Mab-ELISA were equal for cat and Derm. pter. (10 – 102 SQ-U/ml). The range of allergen detection limits for CCIE, assaying dog were 104− 105 SQ-U/ml. The ranges of allergen detection limits for HR were equal for eat and Derm. pter. (10 – 102 SQ-U/ml), and 102− 103 SQ-U/ML for dog. Because of cross-reactivity, a minor degree of interference was observed in the IgE-RI and the HR test for the highest concentration of cat and dog allergens.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1398-9995.1991.tb00611.x
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