ISSN:
1076-5174
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
A rapid and sensitive method for the identification of quaternary ammonium and tertiary sulfonium compounds is described. The method utilizes plasma desorption mass spectrometry (PDMS) in which a 252Cf ionizing source produces fission fragments which interact with the sample to yield ions which are then analyzed by a time-of-flight mass spectrometer. The method was applied to analyses of authentic standards and to the identification of quaternary ammonium and tertiary sulfonium compounds in higher plant leaf extracts purifed by ion-exchange chromatography. Plant species from 14 different families were tested, including species which are known glycinebetaine, β-alaninebetaine, prolinebetaine or β-dimethylsulfoniopropionate accumulators. PDMS results confirmed and extended the known taxonomic distributions of these compounds. Glycinebetaine was found in one species Krameria parvifolia from a family (Krameriaceae) not previously known to accumulate this compound. Pipecolatebetaine was identified in Achillea filipendulina (Asteraceae). The occurrence of prolinebetaine and hydroxyprolinebetaine(s) was confirmed in three species (Lamium maculatum, Stachys byzantina and Phlomis fruticosa) of the tribe Lamieae of the subfamily Lamioideae in the family Lamiaceae, but not in a fourth member of this tribe (Physostegia virginiana). The last species accumulated only trigonelline. The PDMS method was also used to identify trigonelline-deficient Zea mays lines.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jms.1190300817
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