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  • 1
    Digitale Medien
    Digitale Medien
    Reevaluation of triethers as markers for triglycerides during gastric emptying (1977)
    Springer
    Digestive diseases and sciences 22 (1977), S. 449-453 
    Details
    ISSN: 1573-2568
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The validity of using triethers as markers for triglycerides during gastric emptying was investigated. Rats were given a lipid mixture containing [75Se]triether plus [131I]triolein or [3H]triether plus [14C]triolein intragastrically. Similar studies were performed on humans using a homogenized test meal to which was added [75Se]triether plus corn oil. Analysis of gastric contents at various time intervals after administration of the test meal did not show evidence of separation of triglyceride and triether in the stomach. It is discussed that the disappointing results of other workers using [3H]triether may be due to an artifact associated with instability of the tritium-to-triether bond in the preparation used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01071894
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  • 2
    Digitale Medien
    Digitale Medien
    Specificity of immunoassays (1976)
    Springer
    European journal of nuclear medicine 1 (1976), S. 37-48 
    Details
    ISSN: 1619-7089
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The theory of the measurement of the specificity of antigen binding by antibodies is reviewed. The specificity of steroid immunoassay has been investigated using a new solid phase radioimmunoassay system. The effects of plasma proteins on the specificity of the immunoassays have been tested. The methods used and the conclusions reached have been tested with homogeneous antibodies in order to simplify the interpretation of the results obtained. A new criterium of antibody homogeneity is proposed. The importance of correcting all results accurately for “non-specific binding” is emphasised. A new method for plotting radioimmunoassay standard curves is presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00253265
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Specificity of immunoassays (1979)
    Springer
    European journal of nuclear medicine 4 (1979), S. 171-177 
    Details
    ISSN: 1619-7089
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The specificity of immunoassays can be improved by using a second antiserum to bind substances that cross-react. Both theory and practice show that the effectiveness of the procedure is dependent of the complex interplay of the concentrations of the two antibodies, the concentrations of the three antigens involved (the labelled tracer, the antigen whose concentration is to be measured and the substance that cross reacts) and the affinities of the antibodies for these antigens. Measured cross-reactions can frequently be reduced to zero in the most important concentration ranges thereby enabling one to perform assays upon unpurified materials which other wise would not be possible. Limitations of the method are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00620481
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  • 4
    Digitale Medien
    Digitale Medien
    Specificity of immunoassays (1979)
    Springer
    European journal of nuclear medicine 4 (1979), S. 159-170 
    Details
    ISSN: 1619-7089
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Practical aspects of the measurement of the specificity of immunoassay are reviewed. Antibody heterogeneity in an antiserum makes a pragmatic rather than a theoretical approach necessary. A new method for the measurement of immunoassay specificity is described. This method is based on the errors caused by the cross-reacting antigens and is directly relevant to the validity of results obtained by immunoassay methods. The effect of selectively blocking the least specific antibodies in antisera raised against steroid haptens is tested. The practical consequences of these considerations are tested using steroid radioimmunoassay and enzyme-immunoassay.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00620480
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