ISSN:
1573-5028
Keywords:
Agrobacterium rhizogenes
;
binary vector
;
kanamycin resistance
;
transformation
;
secondary metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Co-transfer of Agrobacterium rhizogenes T-DNA and T-DNA from the A. tumefaciens binary vector pBin19 (Bevan, 1984) was studied in detail using Nicotiana rustica. High frequencies of co-transfer of T-DNA's were observed, even when no selection pressure was exerted. Increased levels of pBin19 T-DNA were found in hairy root cultures with selection at higher levels of kanamycin sulphate (50–200 μg ml−1). Several other species were also transformed by A. rhizogenes carrying pBin19 and A. rhizogenes harbouring a different binary factor, pAGS125 (Van den Elzen et al., 1985), was used to transform N. rustica hairy roots to confer hygromycin B resistance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020534
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