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  • 1
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Summary The evolution of sour doughs (SD), prepared using a wheat bran extract as the starting material, and their respective bread doughs (BD) has been investigated by determining the chemical changes which take place in 0.85 mol/L NaCl-soluble nitrogen compounds during fermentation. The quantitative changes determined are: total nitrogen content (TN), primary amine nitrogen (AN) andα-amine nitrogen of free amino acids (AAN) in the total soluble fraction (SN), protein (PSN) and non-protein (NPSN) subfractions. During fermentation,α-amine nitrogen of the free amino acids (AANSN) and peptides (ANNPSN-AANSN), increase in SD and decrease in BD. Changes are larger and faster in peptide nitrogen and even larger during the third and fifth steps. The total protein content (TNPSN) decreases in both SD and BD. Results are discussed in relation to the quality of the bread obtained in each case.
    Notes: Zusammenfassung Es wird die Entwicklung von Sauerteigen mit Weizenkleiextrakt als Starter und ihren entsprechenden Brotteigen durch Bestimmung der chemischen Veranderungen der in 0,85 mol/L NaCl1 slichen Stickstoffverbindungen wahrend der Gärung untersucht. Die quantitativen Veränderungen des Gesamt-Stickstoffgehalts (TN), des primären Aminostickstoffs (AN) und des α-Aminostickstoffs der freien Aminosauren (AAN) in der loslichen Fraktion (SN) und in den Proteinen (PSN) und Nicht-Proteinen (NPNS) der Subfraktionen. Wahrend der Gärung steigt der Anteil desα-Aminostickstoffs in den freien Aminosäuren (AANSN) und Peptiden (ANNPSN-AANSN) in den Sauerteigen (SD) an und vermindert sich in den Brotteigen (BD). Die Veränderungen sind hoher und schneller beim Peptidstickstoff und größer wahrend der dritten und funften Stufe sowohl beim Sauer- als auch beim Brotteig; in beiden nimmt der Proteingehalt (TNPSN) ab. Die Ergebnisse werden bezogen auf die Qualität der hergestellten Brote besprochen.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; 2-deoxyglucose ; 2-deoxyglucose-6 phosphate phosphatase ; DOGR1 ; DOGR2 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Saccharomyces cerevisiae contains two genes (DOGR1 and DOGR2) that are able to confer 2-deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate (2-DOG-6P) phosphatase activity. These enzymes have been purified and characterized. DogR1p shows an optimum pH of 6, an optimum temperature of 30°C and a KM on 2-DOG-6P of 17 mM. DogR2p shows a similar optimum pH, but the optimum temperature is 40°C and it exhibits a KM on 2-DOG-6P of 41 mM. Both enzymes require 10 mM-MgCl2 for maximal activity and they are inhibited by inorganic phosphate.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1195-1202 
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; 2-deoxyglucose ; 2-deoxyglucose-6-phosphate phosphatase ; DOGR1 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated a gene whose expression enables yeast cells to overcome the inhibition of growth produced by the presence of 2-deoxyglucose. The gene contains an open reading frame of 738 bp that may code for a protein of 27 100 Da. Cells carrying this gene contain high levels of a specific 2-deoxyglucose-6-phosphate phosphatase. The expression of this phosphatase is increased by the presence of 2-deoxyglucose and is constant along the growth curve. The sequence reported here has the GenBank accession number U03107.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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