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Osteopontin adhesion receptors on gingival fibroblasts (1995)

D'Errico, J. A. ; Sauk, J. J. ; Prince, C. W. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of periodontal research 30 (1995), S. 0

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ISSN: 1600-0765

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Osteopontin (OPN) promotes attachment and spreading of cells in an RGD dependent fashion, suggesting that OPN interacts with integrins on cell surfaces. Here in, we show that LM-609, a monoclonal antibody to the αvβ3 integrin (a vitronectin receptor), inhibited OPN-mediated attachment of gingival fibroblasts. To characterize the cell surface receptors responsible for this interaction, we performed OPN-sepharose affinity chromatography using detergent extracts of 35S-methionine or l25I-surface labeled gingival fibroblasts. Proteins bound to the OPN-matrix were eluted with EDTA and subjected to SDS-PAGE under reducing conditions. EDTA eluates from both 125I-surface labeled and 35Smethionine labeled extracts demonstrated prominent bands in the 90kDa and 50kDa regions, by both autoradiography and fluorography, respectively. These studies suggest that OPN is associated with other cell surface molecules in addition to αvβ3. Furthermore, these as yet to be characterized proteins, may prove to have a stronger affinity for OPN than αvβ3.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1600-0765.1995.tb01250.x

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Cellular response to well-characterized calcium phosphate coatings and titanium surfaces in vitro (1995)

Ong, J. L. ; Prince, C. W. ; Lucas, L. C.

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 29 (1995), S. 165-172

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: The cellular response to well-characterized Ca-P and Ti surfaces was analyzed. This study indicated that the ion-beam sputtering process could produce a hydroxyapatite-type coating. However, structural alterations such as the incorporation of CO3 and the loss of the OH groups were observed. The Ti surfaces were shown to be predominantly amorphous TiO2. The in vitro cell culture study showed no significant differences in cell number for the Ti and Ca-P surfaces throughout the 12-day study. Furthermore, the initial percentage of protein within the cell layers was significantly lower for cells on Ca-P surfaces compared with the cell layer on Ti surfaces. However, after 3 h incubation, no significant difference in the percentage of protein was observed for the Ti and Ca-P surfaces. © 1995 John Wiley & Sons, Inc.

Additional Material: 8 Ill.