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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nanoparticle research 1 (1999), S. 511-512 
    ISSN: 1572-896X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nanoparticle research 1 (1999), S. 145-145 
    ISSN: 1572-896X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nanoparticle research 1 (1999), S. 115-126 
    ISSN: 1572-896X
    Keywords: nanoparticles ; aerosol charger ; unipolar charging ; Fuchs theory ; experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Notes: Abstract A novel aerosol charger has been developed, which has high efficiency and high throughput especially for nanometer particles in the size range of 3–50 nm. Unipolar charging with high ion concentration and long charging time is used to obtain the high charging efficiency. High throughput is achieved by reducing particle loss within the charger. This is accomplished by directing ion flow and aerosol flow in the same direction and by the use of sheath air flow. The charger configuration is of a longitudinal design – the direction of aerosol stream and ion stream are flowing parallel along the longitudinal axis of the charger. The charger consists of four sections: the inlet zone, the ion production zone, the unipolar charging zone, and the exit zone. In the inlet and ion production zones, unipolar ions are generated using Po210 radioactive sources with an electric field designed to separate the positive and negative ions, and to focus the selected unipolar ions into the core region of the charger. The ions with the selected polarity is then attracted to the charging zone by an uniform electric field created by a series of ring electrodes applied with a linear ramped voltage. Aerosol entering the charger is sheathed with clean gas flow in order to keep the aerosol in the core region. A novel exit design with a reversed electric field is incorporated in order to minimize the charged particles loss. The performance of the charger is first evaluated using computer simulation and then constructed for experimental validation. Experiment data have demonstrated that the charger achieves 90% and 95% charged-particles penetration efficiency and with 22% and 48% extrinsic charging efficiency at 3 and 5 nm particle sizes, respectively. These performance data represent significant improvement, over a factor of 10, compared with the existing chargers.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nanoparticle research 2 (2000), S. 3-4 
    ISSN: 1572-896X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1572-896X
    Keywords: nanometer particles ; fast-response particle analyzer ; jet-engine emissions ; differential mobility analyzer (DMA) ; low-pressure measurement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Notes: Abstract We have developed a fast-response nanometer aerosol size analyzer (nASA) that is capable of scanning 30 size channels between 3 and 100 nm in a total time of 3 s. The analyzer includes a bipolar charger (Po210), an extended-length nanometer differential mobility analyzer (Nano-DMA), and an electrometer (TSI 3068). This combination of components provides particle size spectra at a scan rate of 0.1 s per channel free of uncertainties caused by response-time-induced smearing. The nASA thus offers a fast response for aerosol size distribution measurements in high-concentration conditions and also eliminates the need for applying a de-smearing algorithm to resulting data. In addition, because of its thermodynamically stable means of particle detection, the nASA is useful for applications requiring measurements over a broad range of sample pressures and temperatures. Indeed, experimental transfer functions determined for the extended-length Nano-DMA using the tandem differential mobility analyzer (TDMA) technique indicate the nASA provides good size resolution at pressures as low as 200 Torr. Also, as was demonstrated in tests to characterize the soot emissions from the J85-GE engine of a T-38 aircraft, the broad dynamic concentration range of the nASA makes it particularly suitable for studies of combustion or particle formation processes. Further details of the nASA performance as well as results from calibrations, laboratory tests and field applications are presented below.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nanoparticle research 2 (2000), S. 133-139 
    ISSN: 1572-896X
    Keywords: electrospray of nanodroplets ; gene therapy ; gene transfection ; DNA transfer ; gold nanoparticles ; design of apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Notes: Abstract A novel approach was developed to introduce biological materials into cells for gene transfection and gene therapy applications. The method is based on the technique of electrospraying bio-materials into cells. A prototype apparatus was constructed for a feasibility study. The features of the gene transfector include: (1) A dual-capillary assembly to spray suspensions of biological materials. The outer capillary provided sheathing liquid that controlled the charge level on individual particles without altering the properties of suspensions. (2) An air–CO2 gas mixture was used for suppressing possible corona discharge and kept the same gas composition as those in incubators. (3) The designed chamber enabled the spray to operate at reduced pressure for increasing sprayed particle velocity. In the feasibility study, both suspensions of plasmid and plasmid-coated gold particles were used. The plasmid used was the commercially available Enhanced Green Fluorescent Protein gene. COS-1 cells were used as the target and the liquid media was evacuated immediately prior to the spraying process. Electrospraying was conducted at ambient pressure and the duration was no more than 2 min. After the spray transfection, the media was immediately replaced and the cell samples were returned to the incubator for 36 h. Transgene expression was detected by cellular fluorescence. This technology promises to have great potential for gene transfection and therapy studies.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1572-896X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Type of Medium: Electronic Resource
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