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  • 1
    Electronic Resource
    Electronic Resource
    Expression of Microtubule-Associated Proteins During the Early Stages of Neurite Extension by Brain Neurons Cultured in a Defined Medium (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 47 (1986), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Immunoblotting analysis was used to identify the microtubule-associated proteins (MAPs) present in cultures of mouse brain neurons. Polyclonal antibodies were raised against the two main adult brain MAPs, i.e., MAP2 (300 kDa) and τ (60–70 kDa). Whatever the stage of the culture, which was performed in a defined medium (3 or 6 days), the anti-MAP2 serum detected several high-molecular-weight components (including MAP2) and an entity with 62–65 kDa. Anti-τ revealed essentially a major peak of 48 kDa (young τ) but also slightly cross-reacted with the 62–65 kDa entity. During the culture period (0–6 days) the cells developed progressively a dense neuritic network; the concentration of the different MAPs increased in parallel but at different rates depending on the different species. The in crease in concentration of the high-molecular-weight components occurred before that of 48-kDa τ. This suggests that high-molecular-weight MAPs and 48-kDa τ might be involved respectively in the initiation and elongation of neu-rites. In contrast, and since the main developmental changes in τ composition seen in vivo did not occur during the time course of the culture, this transition might be related to later events of neuronal differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1986.tb00748.x
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  • 2
    Electronic Resource
    Electronic Resource
    POST MORTEM STABILITY AND STORAGE IN THE COLD OF BRAIN ENZYMES (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 32 (1979), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Tyrosine hydroxylase (TH), glutamate-decarboxylase (GAD) and choline acetyltransferase (CAT) were estimated in the striatum of rat brains kept at 20°C or 4°C for various periods of time up to 48 h after death. At 20°C TH and GAD activities decreased up to 4&50% of controls after 48 h; CAT activity was not affected. Maintenance of dead animals at 4°C completely (GAD and CAT) or partially (TH) prevented the decrease in enzyme activities. In a second series of experiments, TH, G A D and CAT activities were measured in striata (tissue or homogenate) stored immediately after death at different temperatures (4°C; -35°C; -70°C) for various time intervals up to 3 months. Storage of striata at 4°C induced a rapid decrease of all enzyme activities with time (GAD 〉 CAT 〉 TH). TH, GAD and CAT activities in striata kept at -35°C or -70°C were fairly stable. However, CAT activity was slightly decreased when the dissected striata were not homogenized; GAD activity was substantially reduced after 3 months at -35°C. Stability of TH, GAD and CAT activities were confirmed in homogenates of human caudate nucleus stored at -70°C for 1 month.If human enzymes behave similarly to the rat enzymes the following conclusions should be drawn: (1) brains should be obtained at autopsy within 8 h after death; (2) placement of dead bodies in the refrigerator should be done as soon as possible; (3) dissected brain structures (preferably as homogenates) should be stored at -70°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1979.tb00370.x
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in Nuclear 3,5,3′-Triiodothyronine Receptor Expression in Rat Dorsal Root Ganglia and Sciatic Nerve During Development: Comparison with Regeneration (1993)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 5 (1993), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The action of the thyroid hormones on responsive cells in the peripheral nervous system requires the presence of nuclear triiodothyronine receptors (NT3R). These nuclear receptors, including both the α and β subtypes of NT3R, were visualized by immunocytochemistry with the specific 2B3 monoclonal antibody. In the dorsal root ganglia (DRG) of rat embryos, NT3R immunoreactivity was first discretely revealed in a few neurons at embryonic day 14 (E14), then strongly expressed by all neurons at E17 and during the first postnatal week; all DRG neurons continued to possess clear NT3R immunostaining, which faded slightly with age. The peripheral glial cells in the DRG displayed a short-lived NT3R immunoreaction, starting at E17 and disappearing from the satellite and Schwann cells by postnatal days 3 and 7 respectively. In the developing sciatic nerve, Schwann cells also exhibited transient NT3R immunoreactivity restricted to a short period ranging from E17 to postnatal day 10; the NT3R immunostaining of the Schwann cells vanished proximodistally along the sciatic nerve, so that the Schwann cells rapidly became free of detectable NT3R immunostaining. However, after the transection or crushing of an adult sciatic nerve, the NT3R immunoreactivity reappeared in the Schwann cells adjacent to the lesion by 2 days, then along the distal segment in which the axons were degenerating, and finally disappeared by 45 days, when the regenerating axons were allowed to re-occupy the distal segment. It is concluded that (1) NT3R expression lasts throughout the life of the DRG neurons; (2) NT3R expression by peripheral glia is restricted to the perinatal period but may be transiently reactivated in Schwann cells after a nerve injury; and (3) cell-cell interaction with axons down-regulates the expression of NT3R by Schwann cells in both growing and regenerating nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.1993.tb00499.x
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical characterization of the uptake and release of [^3H]dopamine by dopaminergic hypothalamic neurons: A developmental study using serum-free medium cultures
    Amsterdam : Elsevier
    Developmental Biology 119 (1987), S. 75-84 
    Details
    ISSN: 0012-1606
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0012-1606(87)90208-9
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 41 (1983), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The biochemical and morphological effects of polyunsaturated fatty acids on fetal brain cells grown in a chemically defined medium were studied. Fetal brain cells were dissociated from mouse cerebral hemispheres taken on the 16th day of gestation. After cells had grown in chemically defined medium for 8 days, the proportion of polyunsaturated fatty acids of cultured cells was only one-half of that observed at day 0 and about 1.5 times less than that of cells grown in serum-supplemented medium. Fatty acid 20:3(n-9) was present in cultured cells grown in either chemically defined or serum-supplemented medium, demonstrating the deficiency of essential fatty acids. The reduced amount of polyunsaturated fatty acids in cells grown in the chemically defined medium was balanced by an increase in monounsaturated fatty acids. The saturated fatty acids were not affected. When added at the seeding time, linoleic, linolenic, arachidonic, or docosahexaenoic acid stimulated the proliferation of small dense cells. Besides, we demonstrate that each of the four fatty acids studied was incorporated into phospholipids. Adding fatty acids of the n-6 series increased the content of n-6 fatty acids in the cells, but also provoked an increase in the n-3 fatty acids. Among several combinations of fatty acids, only 20:4 and 22:6, when added to the culture in a ratio of 2:1, restored a fatty acid profile similar to controls (i.e. in vivo tissue taken at postnatal day 5).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1983.tb00817.x
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  • 6
    Electronic Resource
    Electronic Resource
    Localization of cells in the rat brain expressing fumarylacetoacetate hydrolase, the deficient enzyme in hereditary tyrosinemia type 1
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease 1180 (1993), S. 250-256 
    Details
    ISSN: 0925-4439
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0925-4439(93)90046-4
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  • 7
    Electronic Resource
    Electronic Resource
    The Myotonin-Protein Kinase Phosphorylates Tyrosine Residues in Normal Human Skeletal Muscle
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 199 (1994), S. 89-92 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/bbrc.1994.1198
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