ISSN:
1420-9071
Keywords:
Agrobacterium tumefaciens
;
nopaline plasmid
;
tropical timber tree
;
Fagraea fragrans
;
genetic transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Agrobacterium tumefaciens A208 with nopaline plasmid pTiT37 was used to obtain stem tumors on plantlets ofFagraea fragrans grown in vitro. Bacterial elimination and tissue proliferation were simultaneously achieved by growing tumors on cefatoxime medium. After some tissue growth the shoots regenerated. An examination of these showed the presence of nopaline, indicating genetic transformation by T-DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01960253
Permalink