ISSN:
1398-9995
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Phorbol myristate acetate (PMA) has been widely used to produce interleukin-1 (IL-1) from monocytes (Mø) of various species, including man. Supernatants from cultures of human Mø, stimulated with PMA, 2 × 10−6 M, contained IL-1–like activity, as judged by their high co-mitogenic effect on mitogen-stimulated human T-cells. However, a solution of PMA in culture medium was equally as active as the supernatants. By way of 3H-PMA it was demonstrated that the IL-1–like effect of PMA-induced supernatants was non-dialyzable, was coupled to proteins with a molecular weight ≥ 70,000 Dalton and could be dependent mainly upon the PMA content. Thus, previous reports dealing with basic immunological as well as immunopharmacological aspects of IL-1, must be interpreted with caution if the conclusions have been based on results obtained with crude or high molecular weight forms of PMA-induced IL-1. It could be that PMA can be used as an IL-1 inducer. However, it must first be demonstrated that PMA does not interfere with the detection of IL-1 or, if so, that PMA has been removed sufficiently from the test material to avoid interference. Secondly, the specificity of PMA with respect to IL-1 inducing effect must be demonstrated under endotoxin-free culture conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1398-9995.1985.tb00890.x
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