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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 184 (1959), S. 728-729 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Our experiments consisted in brief of injecting tetracycline (achromysin) into the yolk-sac of embryos eight days old in amounts ranging from O'l to 2*5 mgm. The embryos were examined two, four, six and eight days afterwards. The presence of tetracycline was identified by its characteristic ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 3 (1969), S. 142-150 
    ISSN: 1432-0827
    Keywords: Calcium Distribution ; Tetracycline ; Bone Formation ; Chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La présence de calcium (technique GBHA) dans les cellules du périoste: ostéoblastes, ostéocytes et chondrocytes d'os en cours de développement chez l'embryon de poulet, confirme les résultats deKashiwa (1966) chez le rat. La modification du développement osseux par l'administration de tétracycline indique qu'il y a une corrélation directe entre la quantité de calcium à l'intérieur de ces cellules et le degré de minéralisation des matrices adjacentes. Le calcium du front de calcification de l'os et de la matrice du cartilage anormalement calcifiée qui résulte du traitement à la tétracycline ne semble pas avoir la même capacité à entrer en chélation sous l'action du GBHA, que le calcium d'os plus âgé et de matrice normalement calcifiée. La distribution du matériel positif au GBHA semble correspondre à celle d'un lipide lié, résistant à la pyridine, et déjà décrit.
    Abstract: Zusammenfassung Das Vorkommen von Calcium (GBHA-Technik) in den Zellen des Periosts, in den Osteoblasten, Osteocyten und Chondrocyten des sich entwickelnden Knochens im Hühnerembryo bestätigt die Resultate, welcheKashiwa (1966) bei der Ratte beschrieb. Eine durch Tetracyclingaben hervorgerufene Veränderung der Knochenentwicklung zeigt, daß eine direkte Korrelation zwischen dem Calciumgehalt in diesen Zellen und dem Mineralisationsgrad der sie umgebenden Matrices besteht. Das Calcium an der Calcificationsgrenze des Knochens und in der durch Tetracyclinbehandlung abnormal verkalkten Knochenmatrix scheint sich dadurch zu unterscheiden von älteren Knochen und von normal verkalkender Knorpelmatrix, daß es mit GBHA ein chelat zu bilden vermag. Die Verteilung von GBHA-positivem Material scheint mit der eines früher beschriebenen pyridinresistenten gebundenen Lipids übereinzustimmen.
    Notes: Abstract The presence of calcium (GBHA technique) in the periosteal cells, osteoblasts, osteocytes and chondrocytes of developing chick embryo bone confirms the findings ofKashiwa (1966) in the rat. Modification of bone development by tetracycline administration indicates that there is a direct correlation between the amount of calcium within these cells and the degree of mineralization of the adjacent matrices. The calcium of the calcification front of bone and the abnormally calcified cartilage matrix resulting from tetracycline treatment appears to differ from that of the older bone and normally calcifying cartilage matrix in its ability to chelate with GBHA. The distribution of the GBHA positive material appears to correspond to that of a pyridine-resistant bound lipid previously reported.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 158 (1967), S. 417-431 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The histochemical localization of ribonucleoprotein and mucopolysaccharide components in the developing femurs of normal and tetracycline-treated chick embryos has been described. Tetracycline administration did not appear to affect the formation or character of the fibrous components involved in bone formation, or RNA production. Osteoid was laid down normally but calcification was markedly delayed. The depolymerization of the osteoid, as indicated by metachromasia and PAS staining, which is normally associated with the onset of calcification, was correspondingly delayed. The mucopolysaccharide content of the cartilage model was unchanged by the drug except in the hypertrophic zone. Here an abnormal depolymerization of the matrix occurred gradually, beginning in small areas and progressing to include all the matrix between small groups of cells. Calcification of these regions then took place.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 317-329 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: At intervals from 2 to 13 days of incubation, 2.5 mg of tetracycline hydrochloride was injected into the yolk sac of chick embryos. The femurs and mandibles were examined histologically at intervals between 10 and 17 days of embryonic age.The abnormalities which result include inhibition of mineralization of the developing osteoid trabeculae, retardation of erosion of the long bone cartilage model, and abnormal calcification of the cartilage matrix in the long bones. The major effects on cartilage maturation appear to occur after cellular hypertrophy has taken place and thus are found only in cartilage models which are being replaced by bone.While tetracycline does cause some retardation in the rate of osteoid deposition, the drug appears to affect intramembraneous bone formation in the mandible and femur primarily by retarding or temporarily inhibiting the rate of mineralization of the osteoid matrix.The results of this study indicate that the effects produced by tetracycline on developing bones are dependent upon the concentration of the drug and not upon the time of administration.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 105 (1949), S. 281-297 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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